Karsten Weis
YOU?
Author Swipe
View article: Timing of transcription controls the selective translation of newly synthesized mRNAs during acute environmental stress
Timing of transcription controls the selective translation of newly synthesized mRNAs during acute environmental stress Open
When cells encounter stress, they rapidly mount an adaptive response by switching from pro-growth to stress-responsive gene expression programs. How cells selectively silence pre-existing, pro-growth transcripts yet efficiently translate t…
View article: Development of CD371 shielded hematopoietic stem cell grafts for protection against AML-directed immunotherapies
Development of CD371 shielded hematopoietic stem cell grafts for protection against AML-directed immunotherapies Open
CAR T cells are a promising immunotherapy for AML. However, a major obstacle in the success of CAR T cells for AML has been that the most commonly evaluated AML surface antigens are expressed on normal hematopoietic stem and progenitor cel…
View article: The environmental stress response controls the biophysical properties of the cytoplasm and is critical for survival in quiescence
The environmental stress response controls the biophysical properties of the cytoplasm and is critical for survival in quiescence Open
All organisms have evolved survival strategies to cope with changes in environmental conditions. Nutrient deprivation, one of the most frequently encountered stresses in nature, causes haploid budding yeast to enter a reversible state of n…
View article: RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibition
RAF-independent MEK mutations drive refractory histiocytic neoplasms but respond to ERK inhibition Open
Histiocytic neoplasms are clonal disorders of the monocyte/macrophage lineage defined by mutations activating mitogen-activated protein kinase (MAPK) signaling. Recently, the MEK1/2 inhibitor cobimetinib was FDA-approved for patients with …
View article: A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments
A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments Open
Summary Nuclear Pore Complexes (NPCs) are key gateways to the nucleus and major organizers of genome architecture. Despite their importance, it is still not fully understood how NPCs are formed and degraded. Tools to track specific NPCs ov…
View article: A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments
A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments Open
Summary Nuclear Pore Complexes (NPCs) are key gateways to the nucleus and major organizers of genome architecture. Despite their importance, it is still not fully understood how NPCs are formed and degraded. Tools to track specific NPCs ov…
View article: Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm
Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm Open
The organization and biophysical properties of the cytoplasm influence all cellular reactions, including molecular interactions and the mobility of biomolecules. The cytoplasm does not behave like a simple fluid but is a densely crowded an…
View article: Detecting Nuclear Pore Complex assembly in living cells
Detecting Nuclear Pore Complex assembly in living cells Open
The formation of nuclear pore complexes (NPCs) - vital gateways regulating nuclear-cytoplasmic transport - is a highly orchestrated process requiring the integration of hundreds of nucleoporins into the nuclear envelope. A major challenge …
View article: A conserved mechanism of membrane fusion in nuclear pore complex assembly
A conserved mechanism of membrane fusion in nuclear pore complex assembly Open
The nuclear pore complex (NPC) forms a large channel that spans the double lipid bilayer of the nuclear envelope and is the central gateway for macromolecular transport between the nucleus and cytoplasm in eukaryotes. NPC biogenesis requir…
View article: A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments
A tool to pulse-label yeast Nuclear Pore Complexes in imaging and biochemical experiments Open
Summary Nuclear Pore Complexes (NPCs) are key gateways to the nucleus and major organizers of genome architecture. Despite their importance, it is still not fully understood how NPCs are formed and degraded. Tools to track specific NPCs ov…
View article: Limited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells
Limited proteolysis-coupled mass spectrometry captures proteome-wide protein structural alterations and biomolecular condensation in living cells Open
The function of a protein is determined by its structure, which may change dynamically in response to post-translational modifications, interaction with other molecules, or environmental factors like temperature. Limited proteolysis-couple…
View article: The dark side of fluorescent protein tagging—the impact of protein tags on biomolecular condensation
The dark side of fluorescent protein tagging—the impact of protein tags on biomolecular condensation Open
Biomolecular condensation has emerged as an important mechanism to control various cellular processes through the formation of membraneless organelles. Fluorescent protein tags have been extensively used to study the formation and the prop…
View article: The scramblases VMP1 and TMEM41B are required for primitive endoderm specification by targeting WNT signaling
The scramblases VMP1 and TMEM41B are required for primitive endoderm specification by targeting WNT signaling Open
The ER-resident proteins VMP1 and TMEM41B share a conserved DedA domain, which confers lipid scramblase activity. Loss of either gene results in embryonic lethality in mice and defects in autophagy and lipid droplet metabolism. To investig…
View article: The dark side of fluorescent protein tagging – the impact of protein tags on biomolecular condensation
The dark side of fluorescent protein tagging – the impact of protein tags on biomolecular condensation Open
Biomolecular condensation has emerged as an important mechanism to control various cellular processes through the formation of membraneless organelles. Fluorescent protein tags have been extensively used to study the formation and the prop…
View article: Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm
Polysomes and mRNA control the biophysical properties of the eukaryotic cytoplasm Open
The organization and biophysical properties of the cytoplasm influence all cellular reactions, including molecular interactions and the mobility of biomolecules. It has become clear that the cytoplasm does not behave like a simple fluid bu…
View article: Development of CAR T Cells Targeting U5 snRNP200 for the Treatment of Acute Myeloid and B-Lymphoid Leukemias
Development of CAR T Cells Targeting U5 snRNP200 for the Treatment of Acute Myeloid and B-Lymphoid Leukemias Open
Currently there are no effective immunotherapies for AML outside of allogeneic transplant. This challenge is, in part, due to a lack of known antigens unique to AML and not present on normal hematopoietic stem or progenitor cells (HSPCs). …
View article: Timing of transcription controls the selective translation of newly synthesized mRNAs during acute environmental stress
Timing of transcription controls the selective translation of newly synthesized mRNAs during acute environmental stress Open
When cells encounter environmental stress, they rapidly mount an adaptive response by switching from pro-growth to stress-responsive gene expression programs. It is poorly understood how cells selectively silence pre-existing, pro-growth t…
View article: Glucose stress causes mRNA retention in nuclear Nab2 condensates
Glucose stress causes mRNA retention in nuclear Nab2 condensates Open
Nuclear mRNA export via nuclear pore complexes is an essential step in eukaryotic gene expression. Although factors involved in mRNA transport have been characterized, a comprehensive mechanistic understanding of this process and its regul…
View article: The scramblases VMP1 and TMEM41b are required for primitive endoderm specification by targeting WNT signaling
The scramblases VMP1 and TMEM41b are required for primitive endoderm specification by targeting WNT signaling Open
The ER resident proteins VMP1 and TMEM41b share a conserved DedA domain, which confers lipid scramblase activity. Loss of either gene results in embryonic lethality in mice and defects in autophagy and lipid droplet metabolism. We set out …
View article: CAG-Repeat RNA Hairpin Folding and Recruitment to Nuclear Speckles with a Pivotal Role of ATP as a Cosolute
CAG-Repeat RNA Hairpin Folding and Recruitment to Nuclear Speckles with a Pivotal Role of ATP as a Cosolute Open
A hallmark of Huntington's disease (HD) is a prolonged polyglutamine sequence in the huntingtin protein and, correspondingly, an expanded cytosine, adenine, and guanine (CAG) triplet repeat region in the mRNA. A majority of studies investi…
View article: An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae
An amphipathic helix in Brl1 is required for nuclear pore complex biogenesis in S. cerevisiae Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity
Dynamic arrest and aging of biomolecular condensates are modulated by low-complexity domains, RNA and biochemical activity Open
Biomolecular condensates require suitable control of material properties for their function. Here we apply Differential Dynamic Microscopy (DDM) to probe the material properties of an in vitro model of processing bodies consisting of out-o…
View article: The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA–Protein Condensates
The Role of DEAD-Box ATPases in Gene Expression and the Regulation of RNA–Protein Condensates Open
DEAD-box ATPases constitute a very large protein family present in all cells, often in great abundance. From bacteria to humans, they play critical roles in many aspects of RNA metabolism, and due to their widespread importance in RNA biol…
View article: An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i>
An amphipathic helix in Brl1 is required for membrane fusion during nuclear pore complex biogenesis in <i>S. cerevisiae</i> Open
The nuclear pore complex (NPC) is the central portal for macromolecular exchange between the nucleus and cytoplasm. In all eukaryotes, NPCs assemble into an intact nuclear envelope (NE) during interphase, but the process of NPC biogenesis …
View article: Glucose stress causes mRNA retention in nuclear Nab2 condensates
Glucose stress causes mRNA retention in nuclear Nab2 condensates Open
Summary Nuclear mRNA export via nuclear pore complexes is an essential step in eukaryotic gene expression. Although factors involved in mRNA transport have been characterized, a comprehensive mechanistic understanding of this process and i…
View article: Dead or alive: DEAD-box ATPases as regulators of ribonucleoprotein complex condensation
Dead or alive: DEAD-box ATPases as regulators of ribonucleoprotein complex condensation Open
DEAD-box ATPase proteins are found in all clades of life and have been associated with a diverse array of RNA-processing reactions in eukaryotes, bacteria and archaea. Their highly conserved core enables them to bind RNA, often in an ATP-d…