Katerina Kourentzi
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View article: Lateral Flow Assay to Detect Carbonic Anhydrase IX in Seromas of Breast Implant-Associated Anaplastic Large Cell Lymphoma
Lateral Flow Assay to Detect Carbonic Anhydrase IX in Seromas of Breast Implant-Associated Anaplastic Large Cell Lymphoma Open
Background/Objective: Breast implant-associated anaplastic large cell lymphoma (BIA-ALCL) has affected more than 1700 women with textured breast implants. About 80% of patients present with fluid (seroma) around their implant. BIA-ALCL can…
View article: Development of a rapid antigen-based lateral flow assay for tick-borne spotted fever rickettsioses
Development of a rapid antigen-based lateral flow assay for tick-borne spotted fever rickettsioses Open
Tick-borne spotted fever rickettsioses (SFRs) continue to cause severe illness and death in otherwise-healthy individuals due to lack of a timely and reliable diagnostic laboratory test. We recently identified a diagnostic biomarker for SF…
View article: Lateral flow assay-based detection of nuclear fusion oncoprotein: implications for screening of acute promyelocytic leukemia
Lateral flow assay-based detection of nuclear fusion oncoprotein: implications for screening of acute promyelocytic leukemia Open
A lateral flow assay integrated with leukocyte isolation and on-filter cell lysis for the detection of PML–RARα nuclear fusion oncoprotein at point-of-care.
View article: Fluorescent Reporter‐Based Fiber Optic Probe for Continuous Detection of Antibodies
Fluorescent Reporter‐Based Fiber Optic Probe for Continuous Detection of Antibodies Open
Measurement of antibody and antibody fusion protein concentration is vital for process development and manufacturing. Continuous, in‐line monitoring of antibody concentration could be useful in a variety of applications, such as controllin…
View article: IL-9 Is a Biomarker of BIA-ALCL Detected Rapidly by Lateral Flow Assay
IL-9 Is a Biomarker of BIA-ALCL Detected Rapidly by Lateral Flow Assay Open
Background A delayed seroma around breast implants is the most common clinical presentation of breast implant–associated anaplastic large cell lymphoma (BIA-ALCL). Interleukin-9 (IL-9), IL-10, and IL-13 concentrations are significantly hig…
View article: Correction: Smartphone-read phage lateral flow assay for point-of-care detection of infection
Correction: Smartphone-read phage lateral flow assay for point-of-care detection of infection Open
Correction for ‘Smartphone-read phage lateral flow assay for point-of-care detection of infection’ by Maede Chabi, et al. , Analyst , 2023, 148 , 839–848, https://doi.org/10.1039/D2AN01499H.
View article: Smartphone-readable RPA-LFA for the high-sensitivity detection of Leishmania kDNA using nanophosphor reporters
Smartphone-readable RPA-LFA for the high-sensitivity detection of Leishmania kDNA using nanophosphor reporters Open
Early diagnosis of infectious diseases improves outcomes by enabling earlier delivery of effective treatment, and helps prevent further transmission by undiagnosed persons. We demonstrated a proof-of-concept assay combining isothermal ampl…
View article: A review of rapid food safety testing: using lateral flow assay platform to detect foodborne pathogens
A review of rapid food safety testing: using lateral flow assay platform to detect foodborne pathogens Open
The detrimental impact of foodborne pathogens on human health makes food safety a major concern at all levels of production. Conventional methods to detect foodborne pathogens, such as live culture, high-performance liquid chromatography, …
View article: Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays
Glowstick-inspired smartphone-readable reporters for sensitive, multiplexed lateral flow immunoassays Open
The COVID-19 pandemic has increased demand for point-of-care (POC) screening tests such as lateral flow assays (LFAs) and highlighted the need for sensitive and cost-effective POC diagnostic platforms. Here, we demonstrate an LFA platform …
View article: Expression and Characterization of Intein-Cyclized Trimer of Staphylococcus aureus Protein A Domain Z
Expression and Characterization of Intein-Cyclized Trimer of Staphylococcus aureus Protein A Domain Z Open
Staphylococcus aureus protein A (SpA) is an IgG Fc-binding virulence factor that is widely used in antibody purification and as a scaffold to develop affinity molecules. A cyclized SpA Z domain could offer exopeptidase resistance, reduced …
View article: Smartphone-read phage lateral flow assay for point-of-care detection of infection
Smartphone-read phage lateral flow assay for point-of-care detection of infection Open
A new, universal class of M13 phage-based LFA reporters was validated in the point-of-care detection of SARS-CoV-2 nucleoprotein in clinical samples using a smartphone-based reader.
View article: “Glow ELISA”: sensitive immunoassay with minimal equipment and stable reagents
“Glow ELISA”: sensitive immunoassay with minimal equipment and stable reagents Open
Glow enzyme-linked immunosorbent assay (glow ELISA) uses inexpensive and shelf-stable glow stick reagents to chemically excite fluorescent reporters, obviating the need for excitation light sources, filters, and complex optics.
View article: A novel technology for home monitoring of lupus nephritis that tracks the pathogenic urine biomarker ALCAM
A novel technology for home monitoring of lupus nephritis that tracks the pathogenic urine biomarker ALCAM Open
Introduction The gold standard for diagnosis of active lupus nephritis (ALN), a kidney biopsy, is invasive with attendant morbidity and cannot be serially repeated. Urinary ALCAM (uALCAM) has shown high diagnostic accuracy for renal pathol…
View article: Flash Characterization of Smartphones Used in Point-of-Care Diagnostics
Flash Characterization of Smartphones Used in Point-of-Care Diagnostics Open
Rapidly growing interest in smartphone cameras as the basis of point-of-need diagnostic and bioanalytical technologies increases the importance of quantitative characterization of phone optical performance under real-world operating condit…
View article: Expanding the chemical diversity of M13 bacteriophage
Expanding the chemical diversity of M13 bacteriophage Open
Bacteriophage M13 virions are very stable nanoparticles that can be modified by chemical and genetic methods. The capsid proteins can be functionalized in a variety of chemical reactions without loss of particle integrity. In addition, Gen…
View article: Smartphone-Read Phage Lateral Flow Assay for Point-of-Care Detection of SARS-CoV-2 Infection
Smartphone-Read Phage Lateral Flow Assay for Point-of-Care Detection of SARS-CoV-2 Infection Open
The COVID-19 pandemic has highlighted the urgent need for sensitive, affordable, and widely-accessible testing at the point-of-care. Here we report the development of a sensitive chemiluminescence-based smartphone-readable lateral flow ass…
View article: Continuous Monitoring of IgG using Immobilized Fluorescent Reporters
Continuous Monitoring of IgG using Immobilized Fluorescent Reporters Open
In the manufacture of therapeutic monoclonal antibodies (mAbs), the clarified cell culture fluid is typically loaded onto an initial protein A affinity capture column. Imperfect mass transfer and loading to maximum capacity can risk antibo…
View article: Antibody mix-and-read assays based on fluorescence intensity probes
Antibody mix-and-read assays based on fluorescence intensity probes Open
Antibodies and Fc fusion proteins are a rapidly growing class of pharmaceuticals. Cell culture and purification process development and operation require frequent measurement of product concentrations, commonly by complex enzyme-linked imm…
View article: Isocratic reporter-exclusion immunoassay using restricted-access adsorbents
Isocratic reporter-exclusion immunoassay using restricted-access adsorbents Open
We introduce analyte-dependent exclusion of reporter reagents from restricted-access adsorbents as the basis of an isocratic reporter-exclusion immunoassay for viruses, proteins, and other analytes.
View article: Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR
Neutral DNA–avidin nanoparticles as ultrasensitive reporters in immuno-PCR Open
We have demonstrated a novel immuno-PCR-based diagnostic platform which uses self-assembled ultra-detectable DNA–avidin nanoparticles stabilized with poly(ethylene glycol) for the ultrasensitive detection of protein analytes.