Katrin G. Heinze
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View article: A 3D Biofabricated Disease Model Mimicking the Brain Extracellular Matrix Suitable to Characterize Intrinsic Neuronal Network Alterations in the Presence of a Breast Tumor Disseminated to the Brain
A 3D Biofabricated Disease Model Mimicking the Brain Extracellular Matrix Suitable to Characterize Intrinsic Neuronal Network Alterations in the Presence of a Breast Tumor Disseminated to the Brain Open
A 3D cellular breast‐to‐brain tumor model is developed using thiolated‐hyaluronic‐acid‐based hydrogel cross‐linked with extracellular matrix (ECM) proteins collagen type IV, laminin‐211, and fibronectin to mimic the native‐brain ECM. The 3…
View article: Dissecting antibody responses to cardiac receptors in patients with myocardial infarction
Dissecting antibody responses to cardiac receptors in patients with myocardial infarction Open
Myocardial infarction (MI) triggers the production of heart-reactive serum antibodies, but their antigen specificities and impact on cardiomyocyte function remain poorly understood. Using single-cell RNA and B cell receptor sequencing (scR…
View article: Network Controllability Reveals Key Mitigation Points for Tumor-Promoting Signaling in Tumor-Educated Platelets
Network Controllability Reveals Key Mitigation Points for Tumor-Promoting Signaling in Tumor-Educated Platelets Open
Therapeutic strategies targeting “tumor-educated platelets” (TEPs) and platelet–tumor interactions by key signaling pathways (ITAM, P2Y12) may reduce metastasis and cancer. Using a TEP gene expression dataset originally created to study sw…
View article: Network Controllability Reveals Key Mitigation Points for Tumor-Promoting Signaling in Tumor-Educated Platelets
Network Controllability Reveals Key Mitigation Points for Tumor-Promoting Signaling in Tumor-Educated Platelets Open
Therapeutic strategies targeting “tumor-educated platelets” (TEPs) and platelet-tumor interactions by key signaling pathways (ITAM, P2Y12) may reduce metastasis and cancer. Using a TEP gene expression dataset originally created to study sw…
View article: Integration of customizable 3D printed mixing printheads for controlled and tuneable material and stiffness gradient fabrication with high cell viability in extrusion-based bioprinting
Integration of customizable 3D printed mixing printheads for controlled and tuneable material and stiffness gradient fabrication with high cell viability in extrusion-based bioprinting Open
The fabrication of native tissue-like structures with gradual transitions in material properties, cell types and growth factors remains a major challenge in biofabrication. Particularly the complex hierarchical organization, present in liv…
View article: Deep learning-driven automated high-content dSTORM imaging with a scalable open-source toolkit
Deep learning-driven automated high-content dSTORM imaging with a scalable open-source toolkit Open
Super-resolution microscopy offers the ability to visualize molecular structures in biological samples with unprecedented detail. However, the full potential of these techniques is often hindered by a lack of automated, user-independent wo…
View article: Super-resolution of nodal and paranodal disruption in anti-pan-neurofascin-associated autoimmune nodopathy
Super-resolution of nodal and paranodal disruption in anti-pan-neurofascin-associated autoimmune nodopathy Open
Introduction In autoimmune nodopathies, autoantibodies target the nodes of Ranvier, impairing saltatory nerve conduction. Understanding the impact of autoantibody binding on protein assembly is crucial for gaining insights into the pathoge…
View article: 3D In Vitro Glioma‐Neuron‐Astrocyte Biomimetic Composites Recapitulate Key Molecular Mechanisms Linked to Glioblastoma Multiforme Pathophysiology
3D In Vitro Glioma‐Neuron‐Astrocyte Biomimetic Composites Recapitulate Key Molecular Mechanisms Linked to Glioblastoma Multiforme Pathophysiology Open
Glioblastoma multiforme is the most devastating brain tumor without cure. Although in vitro and in vivo research on glioblastoma multiforme have demonstrated its complexity, including interactions with brain cells and the tumor microenviro…
View article: tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling
tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling Open
Summary We introduce software for reading, writing and processing fluorescence single-molecule and image spectroscopy data and developing analysis pipelines to unify various spectroscopic analysis tools. Our software can be used for proces…
View article: Impact of Polymorphic Microfibers for Establishment of Neuronal Model
Impact of Polymorphic Microfibers for Establishment of Neuronal Model Open
The biological and mechanical environment of cells is better mimicked in 3D compared to 2D cell cultures. However, creating accurate 3D cell culture models particularly for ultra‐soft tissues like brain or spinal cord is challenging since …
View article: Thrombo-inflammation analyzed in a validated seven-layer platelet decision model: cellular decisions are tough problems fast and heuristically solved
Thrombo-inflammation analyzed in a validated seven-layer platelet decision model: cellular decisions are tough problems fast and heuristically solved Open
Decisions in biology happen fast and are driven by evolution to optimize survival chances. In platelets, this is achieved by organizing signaling cascades into rapid decision-funnels with modulatory crosstalk. We show that network decision…
View article: A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy
A vascularized breast cancer spheroid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy Open
View article: Semaphorin 7A coordinates neutrophil response during pulmonary inflammation and sepsis
Semaphorin 7A coordinates neutrophil response during pulmonary inflammation and sepsis Open
Pulmonary defense mechanisms are critical for host integrity during pneumonia and sepsis. This defense is fundamentally dependent on the activation of neutrophils during the innate immune response. Recent work has shown that semaphorin 7A …
View article: tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling
tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling Open
We introduce software for reading, writing and processing fluorescence single-molecule and image spectroscopy data and developing analysis pipelines that unifies various spectroscopic analysis tools. Our software can be used for processing…
View article: A humanized breast cancer microorganoid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy
A humanized breast cancer microorganoid platform for the ranked evaluation of tumor microenvironment-targeted drugs by light sheet fluorescence microscopy Open
Targeting the supportive tumor microenvironment (TME) is an approach of high interest in cancer drug development. However, assessing TME-targeted drug candidates presents a unique set of challenges: Adequate assays need to recreate the TME…
View article: Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease
Role of the Glycine Receptor β Subunit in Synaptic Localization and Pathogenicity in Severe Startle Disease Open
Startle disease is due to the disruption of recurrent inhibition in the spinal cord. Most common causes are genetic variants in genes ( GLRA1 , GLRB ) encoding inhibitory glycine receptor (GlyR) subunits. The adult GlyR is a heteropentamer…
View article: Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism
Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism Open
Xiphophorus fish exhibit a clear phenotypic polymorphism in puberty onset and reproductive strategies of males. In X. nigrensis and X. multilineatus , puberty onset is genetically determined and linked to a melanocortin 4 receptor (Mc4r) p…
View article: Platelet-derived microRNAs play a pivotal role in cardiac remodeling after myocardial ischemia and reperfusion
Platelet-derived microRNAs play a pivotal role in cardiac remodeling after myocardial ischemia and reperfusion Open
Background Platelets can infiltrate ischemic myocardium and are increasingly recognized as critical regulators of inflammatory processes during myocardial ischemia and reperfusion (I/R). Platelets contain a broad repertoire of miRNAs, whic…
View article: Direct 3D‐Bioprinting of hiPSC‐Derived Cardiomyocytes to Generate Functional Cardiac Tissues
Direct 3D‐Bioprinting of hiPSC‐Derived Cardiomyocytes to Generate Functional Cardiac Tissues Open
3D‐bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, a method is presented to directly 3D‐bioprint human induced …
View article: Platelet‐derived lipids promote insulin secretion of pancreatic β cells
Platelet‐derived lipids promote insulin secretion of pancreatic β cells Open
View article: A key role for platelet GPVI in neutrophil recruitment, migration, and NETosis in the early stages of acute lung injury
A key role for platelet GPVI in neutrophil recruitment, migration, and NETosis in the early stages of acute lung injury Open
Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are associated with high morbidity and mortality. Excessive neutrophil infiltration into the pulmonary airspace is the main cause for the acute inflammation and lung in…
View article: Direct 3D-bioprinting of hiPSC-derived cardiomyocytes to generate functional cardiac tissues
Direct 3D-bioprinting of hiPSC-derived cardiomyocytes to generate functional cardiac tissues Open
3D-bioprinting is a promising technology to produce human tissues as drug screening tool or for organ repair. However, direct printing of living cells has proven difficult. Here, we present a method to directly 3D-bioprint human induced pl…
View article: Super-resolution imaging pinpoints the periodic ultrastructure at the human node of Ranvier and its disruption in patients with polyneuropathy
Super-resolution imaging pinpoints the periodic ultrastructure at the human node of Ranvier and its disruption in patients with polyneuropathy Open
The node of Ranvier is the key element in saltatory conduction along myelinated axons, but its specific protein organization remains elusive in the human species. To shed light on nanoscale anatomy of the human node of Ranvier in health an…
View article: Platelet glycoprotein V spatio-temporally controls fibrin formation
Platelet glycoprotein V spatio-temporally controls fibrin formation Open
View article: Image-based modeling of vascular organization to evaluate anti-angiogenic therapy
Image-based modeling of vascular organization to evaluate anti-angiogenic therapy Open
View article: Platelet-Derived MicroRNAs Regulate Cardiac Remodeling After Myocardial Ischemia
Platelet-Derived MicroRNAs Regulate Cardiac Remodeling After Myocardial Ischemia Open
Background: Platelets can infiltrate ischemic myocardium and are increasingly recognized as critical regulators of inflammatory processes during myocardial ischemia and reperfusion (I/R). Platelets contain a broad repertoire of microRNAs (…
View article: Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism
Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism Open
Example dataset and scripts used in the analysis of PIE-based FRET-FLIM data of the Mc4r receptor.
View article: Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism
Dimerization of melanocortin 4 receptor controls puberty onset and body size polymorphism Open
Example dataset and scripts used in the analysis of PIE-based FRET-FLIM data of the Mc4r receptor.
View article: Imaged-based modeling of vascular organization to evaluate anti-angiogenic therapy
Imaged-based modeling of vascular organization to evaluate anti-angiogenic therapy Open
In tumor therapy anti-angiogenic approaches have the potential to increase the efficacy of a wide variety of subsequently or co-administered agents, possibly by improving or normalizing the defective tumor vasculature. Successful implement…
View article: Anti-pan-neurofascin antibodies induce subclass-related complement activation and nodo-paranodal damage
Anti-pan-neurofascin antibodies induce subclass-related complement activation and nodo-paranodal damage Open
Autoimmune neuropathy associated with antibodies against pan-neurofascin is a new subtype of nodo-paranodopathy. It is relevant because it is associated with high morbidity and mortality. Affected patients often require intensive care unit…