Kazuhiko Imakawa
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View article: Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro
Toll-like receptor 2 activation of early divided bovine embryo promotes its viability and development competence in vitro Open
View article: Circulating Cell‐Free DNA of Bovine Leukemia Virus: A Promising Biomarker for Enzootic Bovine Leukosis
Circulating Cell‐Free DNA of Bovine Leukemia Virus: A Promising Biomarker for Enzootic Bovine Leukosis Open
Bovine leukemia virus (BLV) causes Enzootic bovine leukosis (EBL) in approximately 1%–5% of infected cattle after a long latent period. Few biomarkers effectively distinguish non‐EBL from EBL cattle. Given the rapid turnover of tumor cells…
View article: Biological Aging and Uterine Fibrosis in Cattle: Reproductive Trade-Offs from Enhanced Productivity
Biological Aging and Uterine Fibrosis in Cattle: Reproductive Trade-Offs from Enhanced Productivity Open
Reproductive efficiency in cattle remains sub-optimal, with pregnancy rates often below 50%, despite fertilization rates approaching 100%, indicating that implantation failure and/or early embryonic loss are major limiting factors. This di…
View article: Epithelial–Mesenchymal Transitions Leading to Conceptus Adhesion in Ruminants: Early Pregnancy Events in Cattle
Epithelial–Mesenchymal Transitions Leading to Conceptus Adhesion in Ruminants: Early Pregnancy Events in Cattle Open
Trophoblast–endometrium interactions play a critical role in the processes of conceptus elongation, attachment, and adhesion, followed by placental development during early pregnancy in ruminants. The attachment between uterine epithelium …
View article: Platelet secretions exert anti-inflammatory effects in vitro on neutrophils and uterine epithelia in cattle: a possible role in amplifying the uterine immune network toward pregnancy
Platelet secretions exert anti-inflammatory effects in vitro on neutrophils and uterine epithelia in cattle: a possible role in amplifying the uterine immune network toward pregnancy Open
Background Platelet derivatives improve the uterine immune environment and increase pregnancy success in humans and animals. Platelet-conditioned media (PCM) contain all molecules derived from platelets in vitro (platelet secretions). The …
View article: A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis
A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis Open
Enzootic bovine leukosis (EBL), although eradicated in some European countries, is still the most common neoplastic disease of cattle, caused by the bovine leukemia virus (BLV). During the progression of EBL, BLV-infected cells clonally ex…
View article: Ovine conceptuses express phospholipase inhibitory genes on days 14-15 of pregnancy, interacting with IFNT pathways
Ovine conceptuses express phospholipase inhibitory genes on days 14-15 of pregnancy, interacting with IFNT pathways Open
In mammals, various molecules are involved in biochemical interaction between conceptus and endometrium for pregnancy recognition and establishment. In ruminants, interferon tau (IFNT) is the pregnancy recognition factor; however, IFNT alo…
View article: Buccal Swab Samples from Japanese Brown Cattle Fed with Limonite Reveal Altered Rumen Microbiome
Buccal Swab Samples from Japanese Brown Cattle Fed with Limonite Reveal Altered Rumen Microbiome Open
The areas of the Mount Aso grasslands in Kumamoto, Japan, are the primary location for the breeding of the Kumamoto strain of Japanese Brown cattle (JBRK). Although Aso limonite, deposited by volcanic ash and magma, has been commonly fed t…
View article: Editorial: Developmental biology and endocrine research for a successful pregnancy
Editorial: Developmental biology and endocrine research for a successful pregnancy Open
Citation: Bhattacharjee J, Rolfo A, Barbosa BF, Imakawa K and Ermini L (2024) Editorial: Developmental biology and endocrine research for a successful pregnancy. Front. Endocrinol. 15:1411864. doi: 10.3389/fendo.2024.1411864
View article: Progressive Exaptation of Endogenous Retroviruses in Placental Evolution in Cattle
Progressive Exaptation of Endogenous Retroviruses in Placental Evolution in Cattle Open
Viviparity is made possible by the placenta, a structure acquired relatively recently in the evolutionary history of eutherian mammals. Compared to oviparity, it increases the survival rate of the fetus, owing to the eutherian placenta. Qu…
View article: Progressive Exaptation of Endogenous Retroviruses in Placental Evolution in Cattle
Progressive Exaptation of Endogenous Retroviruses in Placental Evolution in Cattle Open
Viviparity is made possible by the placenta, a structure acquired relatively recently in the evolutionary history of eutherian mammals. Compared to oviparity, it increases the survival rate of the fetus, owing to the eutherian placenta. Qu…
View article: Low proviral load in the Kumamoto strain of Japanese Brown cattle infected with the bovine leukemia virus
Low proviral load in the Kumamoto strain of Japanese Brown cattle infected with the bovine leukemia virus Open
Background The Kumamoto strain of Japanese Brown (JBRK) cattle is a sub-breed of Wagyu and has a different genetic background than that of Japanese Black (JB) cattle. Bovine leukemia virus (BLV) is the pathogen causing enzootic bovine leuk…
View article: Expression of NFIL3 and CEBPA regulated by IFNT induced-PGE2 in bovine endometrial stromal cells during the pre-implantation period
Expression of NFIL3 and CEBPA regulated by IFNT induced-PGE2 in bovine endometrial stromal cells during the pre-implantation period Open
Prostaglandin E2 (PGE2) is considered as a luteoprotective factor, influencing the corpus luteum during the early pregnant period in the bovine species. Cyclic AMP (cAMP) is activated in response to PGE2 and plays a role in many physiologi…
View article: Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period
Global analyses and potential effects of extracellular vesicles on the establishment of conceptus implantation during the peri-implantation period Open
Intrauterine extracellular vesicles (EVs) are involved in establishing proper conceptus-endometrial communication, which is essential for conceptus implantation and subsequent successful placentation. Despite several studies on intrauterin…
View article: Genetic Diversity of <i>BoLA-DRB3</i> in Japanese Brown Cattle
Genetic Diversity of <i>BoLA-DRB3</i> in Japanese Brown Cattle Open
牛伝染性リンパ腫（Enzootic Bovine Leukosis : EBL）は，牛伝染性リンパ腫ウイルス（Bovine Leukemia Virus : BLV）に起因する牛の悪性リンパ腫である。これまでに，ウシの主要組織適合遺伝子複合体（Major Histocompatibility Complex : MHC，ウシにおいてはBovine Leukocyte Antigen : BoLA）のDRB3領域におけるアリルタイプが感染症の抵抗性や感受性に関連していることが…
View article: Clone Dynamics and Its Application for the Diagnosis of Enzootic Bovine Leukosis
Clone Dynamics and Its Application for the Diagnosis of Enzootic Bovine Leukosis Open
Although BLV has been eradicated in some European countries, BLV is still endemic in other countries, including Japan and the United States. EBL causes huge economic damage to the cattle industry.
View article: The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows
The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows Open
View article: Endogenous Retroviruses and Placental Evolution, Development, and Diversity
Endogenous Retroviruses and Placental Evolution, Development, and Diversity Open
The main roles of placentas include physical protection, nutrient and oxygen import, export of gasses and fetal waste products, and endocrinological regulation. In addition to physical protection of the fetus, the placentas must provide im…
View article: New Roles for EVs, miRNA and lncRNA in Bovine Embryo Implantation
New Roles for EVs, miRNA and lncRNA in Bovine Embryo Implantation Open
The sine qua non of new life is fertilization. However, approximately 50% of fertilized eggs/blastocysts in cattle and up to 75% of those from human assisted reproductive procedures fail during the first 3 to 4 weeks of pregnancy, includin…
View article: The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows
The vaginal and uterine blood flow changes during the ovsynch program and its impact on the pregnancy rates in Holstein dairy cows Open
Aim OvSynch is a hormonal protocol for synchronization of estrus and use of artificial insemination (AI) at an optimal time without adverse effects on the ovaries or uterus. This study investigated the use of noninvasive color Doppler ultr…
View article: Phylogenomics and Spatiotemporal Dynamics of Bovine Leukemia Virus Focusing on Asian Native Cattle: Insights Into the Early Origin and Global Dissemination
Phylogenomics and Spatiotemporal Dynamics of Bovine Leukemia Virus Focusing on Asian Native Cattle: Insights Into the Early Origin and Global Dissemination Open
Bovine leukemia virus (BLV), the causative agent of enzootic bovine leukosis, is currently one of the most important pathogens affecting the cattle industry worldwide. Determining where and in which host it originated, and how it dispersed…
View article: Lymphoma cells are circulating in blood of Enzootic Bovine Leukosis and clonality value of virus-infected cells is a useful information for the diagnostic test
Lymphoma cells are circulating in blood of Enzootic Bovine Leukosis and clonality value of virus-infected cells is a useful information for the diagnostic test Open
Bovine leukemia virus (BLV), a retrovirus, causes Enzootic Bovine Leukosis (EBL) in cattle following a latent infection period. The BLV infection results in polyclonal expansion of infected B-lymphocytes and ∼5% of infected cattle develop …
View article: Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers
Characterization of Serum Metabolome and Proteome Profiles Identifies SNX5 Specific for Pregnancy Failure in Holstein Heifers Open
Pregnancy loss predominantly occurs during the first 3–4 weeks due to fertilization failure or early embryonic losses in cattle. Insufficient biochemical communication between conceptus (embryo plus extraembryonic membranes) and endometriu…
View article: Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period
Characterization of lncRNA functioning in ovine conceptuses and endometria during the peri-implantation period Open
In ruminants, RNA-sequence analyses have revealed many characteristics of transcripts expressed in conceptuses (embryo and extraembryonic membrane) during peri-implantation periods; however, lncRNA profiles are yet characterized. In this s…
View article: Epithelial-mesenchymal transition and bi- and multi-nucleated trophoblast cell formation in ovine conceptuses during the peri-implantation period
Epithelial-mesenchymal transition and bi- and multi-nucleated trophoblast cell formation in ovine conceptuses during the peri-implantation period Open
Epithelial-mesenchymal transition (EMT), which is common in cancer metastasis, is also observed during developmental processes such as embryo implantation into the maternal endometrium in humans and rodents. However, this process has not b…
View article: Recent progress of interferon-tau research and potential direction beyond pregnancy recognition
Recent progress of interferon-tau research and potential direction beyond pregnancy recognition Open
Since the discovery of interferon-tau (IFNT) over 30 years ago as the trophectodermal cytokine responsible for the maintenance of the maternal corpus luteum (CL) in ruminants, exhaustive studies have been conducted to identify genes and ge…
View article: Identification of novel candidate factor SNX5 specific for pregnancy failure in Holstein heifers
Identification of novel candidate factor SNX5 specific for pregnancy failure in Holstein heifers Open
Pregnancy loss predominantly occurs during periods between blastocyst hatching and conceptus (embryo plus extraembryonic membranes) implantation to the endometrium in cattle. Insufficient biochemical communication between conceptus and end…
View article: Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions
Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions Open
This study aimed to characterize proteins and exosomal microRNAs (miRNAs) in the uterine flushings (UF) of cows associated with Day 7 (D7) pregnancy using the embryo donor cows of the embryo transfer program. Superovulated cows either were…
View article: A target enrichment high throughput sequencing system for characterization of BLV whole genome sequence, integration sites, clonality and host SNP
A target enrichment high throughput sequencing system for characterization of BLV whole genome sequence, integration sites, clonality and host SNP Open
View article: Supplementary Tables.xlsx
Supplementary Tables.xlsx Open
Metabolome and Proteopme analyses with bovine serum.Table S1: Metabolome analysis with bovine serum on pregnant days 17, 20, and 22.Table S2: Metabolome analysis with bovine serum on cyclic days 0, 7, 14, and 17.Table S3:<…