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View article: A Spatially Resolved Atlas of Alternative Polyadenylation Across 18 Human Tissues and 76 Disease States
A Spatially Resolved Atlas of Alternative Polyadenylation Across 18 Human Tissues and 76 Disease States Open
Alternative polyadenylation (APA) is a key regulator of gene expression and cellular dynamics, yet systematic investigations of spatially resolved APA across diverse human tissues remain limited. Here, we developed SpatialAPA ( https://git…
View article: High-throughput 3D super-resolution imaging in deep tissue
High-throughput 3D super-resolution imaging in deep tissue Open
Fluorescence images obtained with optical microscopes intrinsically suffer from blur and noise, which can be partially reversed by the deconvolution process. However, the deconvolution process is ill-conditioned, leading to a trade-off bet…
View article: FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method
FLUID: a fluorescence-friendly lipid-compatible ultrafast clearing method Open
Many clearing methods achieve high transparency by removing lipid components from tissues, which damages microstructure and limits their application in lipid research. As for methods which preserve lipid, it is difficult to balance transpa…
View article: Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy
Deep tissue super-resolution imaging with adaptive optical two-photon multifocal structured illumination microscopy Open
Visualization of axons and dendritic spines is crucial in neuroscience research. However, traditional microscopy is limited by diffraction-limited resolution and shallow imaging depth, making it difficult to study neuronal dynamics. Two-ph…
View article: High-axial-resolution speckle-free holographic reconstruction via cylindrical quadratic phase method and temporal focusing
High-axial-resolution speckle-free holographic reconstruction via cylindrical quadratic phase method and temporal focusing Open
Holographic techniques enable precise laser manipulation, but suffer from two considerable limitations: speckle and deterioration of axial distribution. Here, we propose a cylindrical quadratic phase (CQP) method with temporal focusing (TF…
View article: Robust and adjustable dynamic scattering compensation for high-precision deep tissue optogenetics
Robust and adjustable dynamic scattering compensation for high-precision deep tissue optogenetics Open
The development of high-precision optogenetics in deep tissue is limited due to the strong optical scattering induced by biological tissue. Although various wavefront shaping techniques have been developed to compensate the scattering, it …
View article: End-to-End Deep-Learning-Based Diagnosis of Benign and Malignant Orbital Tumors on Computed Tomography Images
End-to-End Deep-Learning-Based Diagnosis of Benign and Malignant Orbital Tumors on Computed Tomography Images Open
Determining the nature of orbital tumors is challenging for current imaging interpretation methods, which hinders timely treatment. This study aimed to propose an end-to-end deep learning system to automatically diagnose orbital tumors. A …
View article: A multi-feature deep learning system to enhance glaucoma severity diagnosis with high accuracy and fast speed
A multi-feature deep learning system to enhance glaucoma severity diagnosis with high accuracy and fast speed Open
Glaucoma is the leading cause of irreversible blindness, and the early detection and timely treatment are essential for glaucoma management. However, due to the interindividual variability in the characteristics of glaucoma onset, a single…
View article: FOCMS: An ultrafast optical clearing method with quantified analysis
FOCMS: An ultrafast optical clearing method with quantified analysis Open
Optical clearing technology offers a prospective solution to improve the imaging depth and quality of optical microscopy, but there is still a lack of quantitative standards to accurately evaluate transparency effects so the composition an…
View article: Efficient and compact sol-gel TiO<sub>2</sub> thermo-optic microring resonator modulator
Efficient and compact sol-gel TiO<sub>2</sub> thermo-optic microring resonator modulator Open
Thermo-optic (TO) modulators play an increasingly important role in wavelength routers, lidar, optical computing, and other reconfigurable photonic systems. Highly efficient TO tunable microring resonators (MRRs) were first demonstrated ba…
View article: Multi-Domain Characterization and Rapid Detection Technology for Cocaine and Methamphetamine Acute Abuse based on EEG
Multi-Domain Characterization and Rapid Detection Technology for Cocaine and Methamphetamine Acute Abuse based on EEG Open
Multi-domain characterization and a new fast detection method for acute illegal psychostimulants abuse detection based on waking-state EEG of mice is proposed in this paper. To get corftical electroencephalogram (EEG), three groups of mice…
View article: Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization
Long-term microglial phase-specific dynamics during single vessel occlusion and recanalization Open
Vascular occlusion leading to brain dysfunctions is usually considered evoking microglia-induced inflammation response. However, it remains unclear how microglia interact with blood vessels in the development of vascular occlusion-related …
View article: Multi-Domain Characterization and Rapid Detection Technology for Cocaine and Methamphetamine Acute Abuse based on EEG
Multi-Domain Characterization and Rapid Detection Technology for Cocaine and Methamphetamine Acute Abuse based on EEG Open
Multi-domain characterization and a new fast detection method for acute illegal psychostimulants abuse detection based on waking-state EEG of mice is proposed in this paper. To get corftical electroencephalogram (EEG), three groups of mice…
View article: Three-Dimensional Virtual Optical Clearing With Cycle-Consistent Generative Adversarial Network
Three-Dimensional Virtual Optical Clearing With Cycle-Consistent Generative Adversarial Network Open
High-throughput deep tissue imaging and chemical tissue clearing protocols have brought out great promotion in biological research. However, due to uneven transparency introduced by tissue anisotropy in imperfectly cleared tissues, fluores…
View article: A Structure-Related Fine-Grained Deep Learning System With Diversity Data for Universal Glaucoma Visual Field Grading
A Structure-Related Fine-Grained Deep Learning System With Diversity Data for Universal Glaucoma Visual Field Grading Open
Purpose Glaucoma is the main cause of irreversible blindness worldwide. However, the diagnosis and treatment of glaucoma remain difficult because of the lack of an effective glaucoma grading measure. In this study, we aimed to propose an a…
View article: Two-photon patterned photostimulation with low-power, high-efficiency and reliable single-cell optogenetic control
Two-photon patterned photostimulation with low-power, high-efficiency and reliable single-cell optogenetic control Open
Two-photon optogenetics enables selectively stimulating individual cells for manipulating neuronal ensembles. As the general photostimulation strategy, the patterned two-photon excitation has enabled millisecond-timescale activation for si…
View article: Correction: A biocompatible two-photon absorbing fluorescent mitochondrial probe for deep <i>in vivo</i> bioimaging
Correction: A biocompatible two-photon absorbing fluorescent mitochondrial probe for deep <i>in vivo</i> bioimaging Open
Correction for ‘A biocompatible two-photon absorbing fluorescent mitochondrial probe for deep in vivo bioimaging’ by Lingmin Lin et al. , J. Mater. Chem. B , 2022, DOI: 10.1039/d1tb02040d.
View article: Long-term Microglial Phase-specific Dynamics During Single Vessel Occlusion and Recanalization
Long-term Microglial Phase-specific Dynamics During Single Vessel Occlusion and Recanalization Open
Vascular occlusion leading to brain dysfunctions is usually considered evoking microglia-induced inflammation response. However, it remains unclear how microglia interact with blood vessels in the development of vascular occlusion-related …
View article: Creep Behavior of Near α High Temperature Ti-6.6Al-4.6Sn-4.6Zr-0.9Nb-1.0Mo-0.32Si Alloy
Creep Behavior of Near α High Temperature Ti-6.6Al-4.6Sn-4.6Zr-0.9Nb-1.0Mo-0.32Si Alloy Open
This study focuses on the microstructure characteristics and tensile and creep properties of a near α high temperature Ti-6.6Al-4.6Sn-4.6Zr-0.9Nb-1.0Mo-0.32Si alloy. Microstructure characteristics were quantitatively investigated using opt…
View article: Improved spatial resolution using focal modulation microscopy with a Tai Chi aperture
Improved spatial resolution using focal modulation microscopy with a Tai Chi aperture Open
Focal modulation microscopy (FMM) has gained significant interest in biological imaging. However, the spatial resolution and penetration depth limit the imaging quality of FMM due to the strong scattering background. Here, we introduce FMM…
View article: Deep Learning Assisted Zonal Adaptive Aberration Correction
Deep Learning Assisted Zonal Adaptive Aberration Correction Open
Deep learning (DL) has been recently applied to adaptive optics (AO) to correct optical aberrations rapidly in biomedical imaging. Here we propose a DL assisted zonal adaptive correction method to perform corrections of high degrees of fre…
View article: Fully end-to-end deep-learning-based diagnosis of pancreatic tumors
Fully end-to-end deep-learning-based diagnosis of pancreatic tumors Open
Artificial intelligence can facilitate clinical decision making by considering massive amounts of medical imaging data. Various algorithms have been implemented for different clinical applications. Accurate diagnosis and treatment require …
View article: Learning-based Shack-Hartmann wavefront sensor for high-order aberration detection: erratum
Learning-based Shack-Hartmann wavefront sensor for high-order aberration detection: erratum Open
An erratum is presented to correct the sizes of feature maps of Fig. 1(a) in [ Opt. Express 27 , 33504 ( 2019 ) 10.1364/OE.27.033504 ].