Keiichiro Toda
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View article: Bidirectional quantitative scattering microscopy
Bidirectional quantitative scattering microscopy Open
Quantitative phase microscopy (QPM) and interferometric scattering (iSCAT) microscopy are powerful label-free imaging techniques that are widely used in biomedical applications. Each method, however, possesses distinct limitations: QPM, wh…
View article: Phase retrieval via Zernike phase contrast microscopy with an untrained neural network
Phase retrieval via Zernike phase contrast microscopy with an untrained neural network Open
Zernike’s phase contrast microscopy (PCM) is among the most widely used techniques for observing phase objects, but it lacks a quantitative nature, as it cannot directly provide phase information. Current methods for computationally extrac…
View article: Ludwig-Soret microscopy with vibrational photothermal effect
Ludwig-Soret microscopy with vibrational photothermal effect Open
Vibrational microscopy provides label-free, bond-selective chemical contrast by detecting molecular vibrations, making it invaluable for biomedical research. While conventional methods rely on the direct detection of Raman scattering or in…
View article: Phase retrieval via Zernike phase contrast microscopy with an untrained neural network
Phase retrieval via Zernike phase contrast microscopy with an untrained neural network Open
Zernike's phase contrast microscopy (PCM) is among the most widely used techniques for observing phase objects, but it lacks quantitative nature, as it cannot directly provide phase information. Current methods for computationally extracti…
View article: Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure?
Label-free mid-infrared photothermal microscopy revisits intracellular thermal dynamics: what do fluorescent nanothermometers measure? Open
Fluorescent nanothermometry has revealed pronounced inhomogeneous temperature distributions within cells, establishing the field of single-cell thermal biology. However, this finding has sparked a controversial discussion known as the "10^…
View article: Single-image phase retrieval for off-the-shelf Zernike phase-contrast microscopes
Single-image phase retrieval for off-the-shelf Zernike phase-contrast microscopes Open
Quantitative phase imaging (QPI), such as digital holography, is considered a promising tool in the field of life science due to its noninvasive and quantitative visualization capabilities without the need for fluorescence labeling. Howeve…
View article: Label-free mid-infrared photothermal live-cell imaging beyond video rate
Label-free mid-infrared photothermal live-cell imaging beyond video rate Open
Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in a…
View article: Mid-infrared wide-field nanoscopy
Mid-infrared wide-field nanoscopy Open
Mid-infrared (MIR) spectroscopy is widely recognized as a powerful, non-distractive method for chemical analysis. However, its utility is constrained by a micrometer-scale spatial resolution imposed by the long-wavelength MIR diffraction l…
View article: Quantitative phase retrieval for Zernike phase-contrast microscopy
Quantitative phase retrieval for Zernike phase-contrast microscopy Open
We present a single-image numerical phase retrieval method for Zernike phase-contrast microscopy (ZPM) that addresses halo and shade-off artifacts, as well as the weak phase condition, without requiring hardware modifications. By employing…
View article: Mid-infrared photothermal single-live-cell imaging beyond video rate
Mid-infrared photothermal single-live-cell imaging beyond video rate Open
Advancement in mid-infrared (MIR) technology has led to promising biomedical applications of MIR spectroscopy, such as liquid biopsy or breath diagnosis. On the contrary, MIR microscopy has been rarely used for live biological samples in a…
View article: Adaptive dynamic range shift (ADRIFT) quantitative phase imaging
Adaptive dynamic range shift (ADRIFT) quantitative phase imaging Open
Quantitative phase imaging (QPI) with its high-contrast images of optical phase delay (OPD) maps is often used for label-free single-cell analysis. Contrary to other imaging methods, sensitivity improvement has not been intensively explore…
View article: Adaptive dynamic range shift (ADRIFT) quantitative phase imaging
Adaptive dynamic range shift (ADRIFT) quantitative phase imaging Open
Quantitative phase imaging (QPI) is often used for label-free single cell analysis with its high-contrast images of optical phase delay (OPD) map. Contrary to other imaging methods, sensitivity improvement has not been intensively explored…
View article: Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect
Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect Open
Label-free optical imaging is valuable in biology and medicine because of its non-destructive nature. Quantitative phase imaging (QPI) and molecular vibrational imaging (MVI) are the two most successful label-free methods, providing morpho…
View article: Supplementary document for Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect - 4506719.pdf
Supplementary document for Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect - 4506719.pdf Open
Supplementary Document
View article: Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect
Label-free biochemical quantitative phase imaging with mid-infrared photothermal effect Open
Label-free optical imaging is valuable in biology and medicine with its non-destructive property and reduced optical and chemical damages. Quantitative phase (QPI) and molecular vibrational imaging (MVI) are the two most successful label-f…
View article: Molecular contrast on phase-contrast microscope
Molecular contrast on phase-contrast microscope Open
An optical microscope enables image-based findings and diagnosis on microscopic targets, which is indispensable in many scientific, industrial and medical settings. Majority of microscope users are accustomed to standard benchtop microscop…