Kelda Chia
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View article: Serum albumin binding knob domains engineered within a VH framework III bispecific antibody format and as chimeric peptides
Serum albumin binding knob domains engineered within a VH framework III bispecific antibody format and as chimeric peptides Open
Background Serum albumin binding is an established mechanism to extend the serum half-life of antibody fragments and peptides. The cysteine rich knob domains, isolated from bovine antibody ultralong CDRH3, are the smallest single chain ant…
View article: Zebrafish as a model organism for neurodegenerative disease
Zebrafish as a model organism for neurodegenerative disease Open
The zebrafish is increasingly recognized as a model organism for translational research into human neuropathology. The zebrafish brain exhibits fundamental resemblance with human neuroanatomical and neurochemical pathways, and hallmarks of…
View article: Brain tumours repurpose endogenous neuron to microglia signalling mechanisms to promote their own proliferation
Brain tumours repurpose endogenous neuron to microglia signalling mechanisms to promote their own proliferation Open
Previously we described direct cellular interactions between microglia and AKT1+ brain tumour cells in zebrafish (Chia et al., 2018). However, it was unclear how these interactions were initiated: it was also not clear if they had an impac…
View article: Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains Open
To gain a detailed understanding of the role of different CNS cells during development or the establishment and progression of brain pathologies, it is important to isolate these cells without changing their gene expression profile. The ze…
View article: Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain
Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain Open
It is now clear that microglia and macrophages are present in brain tumors, but whether or how they affect initiation and development of tumors is not known. Exploiting the advantages of the zebrafish (Danio rerio) model, we showed that ma…
View article: Author response: Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain
Author response: Tumor initiating cells induce Cxcr4-mediated infiltration of pro-tumoral macrophages into the brain Open
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Metrics Abstract It is now clear that microglia and macrophages …
View article: A zebrafish live imaging model to study microglia-glioma interactions
A zebrafish live imaging model to study microglia-glioma interactions Open
Glioblastoma multiforme is the most common and deadliest form of brain cancer. Gliomas are highly infiltrated by microglia and macrophages, which have been shown to promote tumour growth and invasion. However, due to limitations of existin…