Ken Laing
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View article: Detection of Candidozyma (formerly Candida) auris from ward wastewater during an outbreak using culture and molecular methods
Detection of Candidozyma (formerly Candida) auris from ward wastewater during an outbreak using culture and molecular methods Open
We report the first detection of Candidozyma (formerly Candida) auris from ward wastewater using culture (sensitivity for detection of ≥1 C. auris patient on ward: 75%) and PCR (sensitivity: 100%). This proof-of-principle study was underta…
View article: Xpert MTB/RIF® cycle threshold as a marker of TB disease severity; Implications for TB treatment stratification
Xpert MTB/RIF® cycle threshold as a marker of TB disease severity; Implications for TB treatment stratification Open
Introduction Recent trials have demonstrated that shortened four-month treatment durations are effective for the majority of people with tuberculosis (TB). However, there is a population of TB patients who require longer treatment duration…
View article: Time Required for Nanopore Whole-Genome Sequencing of <i>Neisseria gonorrhoeae</i> for Identification of Phylogenetic Relationships
Time Required for Nanopore Whole-Genome Sequencing of <i>Neisseria gonorrhoeae</i> for Identification of Phylogenetic Relationships Open
Background Antimicrobial resistance (AMR) in Neisseria gonorrhoeae is a global health challenge. Limitations to AMR surveillance reporting, alongside reduction in culture-based susceptibility testing, has resulted in a need for rapid diagn…
View article: Author response: Effectiveness of rapid SARS-CoV-2 genome sequencing in supporting infection control for hospital-onset COVID-19 infection: Multicentre, prospective study
Author response: Effectiveness of rapid SARS-CoV-2 genome sequencing in supporting infection control for hospital-onset COVID-19 infection: Multicentre, prospective study Open
Article Figures and data Abstract Editor's evaluation Introduction Methods Results Discussion Appendix 1 Data availability References Decision letter Author response Article and author information Metrics Abstract Background: Viral sequenc…
View article: Adjunctive viral cell culture supports treatment decision‐making in patients with secondary humoral immunodeficiency and persistent SARS‐CoV‐2 infection
Adjunctive viral cell culture supports treatment decision‐making in patients with secondary humoral immunodeficiency and persistent SARS‐CoV‐2 infection Open
Persistent Severe Acute Respiratory Syndrome coronavirus-2 (SARS-CoV-2) infection is observed among patients with haematological malignancy, conferring an increased mortality risk.1, 2 Persistent SARS-CoV-2 RNA detection from clinical samp…
View article: Antibiotic Susceptibility, Virulome, and Clinical Outcomes in European Infants with Bloodstream Infections Caused by Enterobacterales
Antibiotic Susceptibility, Virulome, and Clinical Outcomes in European Infants with Bloodstream Infections Caused by Enterobacterales Open
Mortality in neonates with Gram-negative bloodstream infections has remained unacceptably high. Very few data are available on the impact of resistance profiles, virulence factors, appropriateness of empirical treatment and clinical charac…
View article: Early Colorectal Responses to HIV-1 and Modulation by Antiretroviral Drugs
Early Colorectal Responses to HIV-1 and Modulation by Antiretroviral Drugs Open
Innate responses during acute HIV infection correlate with disease progression and pathogenesis. However, limited information is available about the events occurring during the first hours of infection in the mucosal sites of transmission.…
View article: PCR for the detection of pathogens in neonatal early onset sepsis
PCR for the detection of pathogens in neonatal early onset sepsis Open
Real-time PCR has the potential to be a valuable additional tool for the diagnosis of neonatal sepsis.
View article: Management sensitivity, repeatability, and consistency of interpretation of soil health indicators on organic farms in southwestern Ontario
Management sensitivity, repeatability, and consistency of interpretation of soil health indicators on organic farms in southwestern Ontario Open
Assessment tools are needed to evaluate the effect of farming practices on soil health, as there is increasing interest from growers to improve the health of their soils. However, there is limited information on the efficacy of different s…
View article: P665 Rapid sequence typing for antimicrobial resistance surveillance in<i>neisseria gonorrhoeae</i>using whole genome sequencing
P665 Rapid sequence typing for antimicrobial resistance surveillance in<i>neisseria gonorrhoeae</i>using whole genome sequencing Open
Background The Neisseria gonorrhoeae (NG) genome changes by ∼4 single nucleotide polymorphisms (SNP) per genome per year, information which is considered when predicting sexual networks using next generation sequencing to type circulating …
View article: OC 8173 RAPID DETECTION OF <i>MYCOBACTERIUM ULCERANS</i> BY RECOMBINASE POLYMERASE AMPLIFICATION
OC 8173 RAPID DETECTION OF <i>MYCOBACTERIUM ULCERANS</i> BY RECOMBINASE POLYMERASE AMPLIFICATION Open
Background There are no primary measures to prevent people from contracting Buruli ulcer, mainly due to poor understanding of its epidemiology. The current control strategy emphasises early diagnosis and prompt treatment, with the goal of …
View article: Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay
Rapid detection of Mycobacterium ulcerans with isothermal recombinase polymerase amplification assay Open
The Mu-RPA assay represents an alternative to PCR, especially in areas with limited infrastructure.
View article: Microarray analysis of bone marrow lesions in osteoarthritis demonstrates upregulation of genes implicated in osteochondral turnover, neurogenesis and inflammation
Microarray analysis of bone marrow lesions in osteoarthritis demonstrates upregulation of genes implicated in osteochondral turnover, neurogenesis and inflammation Open
Our study is the first to employ detailed histological analysis and microarray techniques to investigate knee OA BMLs. BMLs demonstrated areas of high metabolic activity expressing pain sensitisation, neuronal, extracellular matrix and pro…
View article: Clearance of viable Mycobacterium ulcerans from Buruli ulcer lesions during antibiotic treatment as determined by combined 16S rRNA reverse transcriptase /IS 2404 qPCR assay
Clearance of viable Mycobacterium ulcerans from Buruli ulcer lesions during antibiotic treatment as determined by combined 16S rRNA reverse transcriptase /IS 2404 qPCR assay Open
Current antibiotic therapy for BU is highly successful in most patients but it may be possible to abbreviate treatment to 4 weeks in patients with a low initial bacterial load. On the other hand persistent infection contributes to slow hea…
View article: P1.32 Hand-held rapid whole genome nanopore sequencing to predict<i>neisseria gonorrhoeae</i>antibiotic susceptibility: steps towards clinic based tailored antimicrobial therapy
P1.32 Hand-held rapid whole genome nanopore sequencing to predict<i>neisseria gonorrhoeae</i>antibiotic susceptibility: steps towards clinic based tailored antimicrobial therapy Open
Introduction Next generation sequencing can accurately predict antibiotic susceptibility in Neisseria gonorrhoeae (NG) allowing preservation of first-line treatments in the face of widespread antimicrobial resistance (AMR). The rapid natur…
View article: P1.38 Bacterial populations detected within first void urine samples of symptomatic male patients with urethritis
P1.38 Bacterial populations detected within first void urine samples of symptomatic male patients with urethritis Open
Introduction Applying bacterial 16S rRNA profiling we investigated whether species in first void urine (FVU) differed between men presenting with urethritis symptoms with and without urethral inflammation. Methods 443 patients prospective…
View article: Resistance gene transfer: induction of transducing phage by sub-inhibitory concentrations of antimicrobials is not correlated to induction of lytic phage
Resistance gene transfer: induction of transducing phage by sub-inhibitory concentrations of antimicrobials is not correlated to induction of lytic phage Open
Sub-inhibitory antibiotics induce AMR gene transfer between clinical MRSA, while combination therapy with an inhibiting antibiotic could potentially alter AMR gene packaging into phage particles, reducing AMR transfer. In a continually evo…
View article: O034 Whole genome sequencing to predict<i>Neisseria gonorrhoeae</i>antibiotic susceptibility: toward tailored antimicrobial therapy
O034 Whole genome sequencing to predict<i>Neisseria gonorrhoeae</i>antibiotic susceptibility: toward tailored antimicrobial therapy Open
Background/introduction Absence of genotypic resistance-associated markers in Neisseria gonorrhoeae (NG) may predict antibiotic phenotypic susceptibility (APS). NG Whole genome sequencing (NG-WGS) on nucleic acid amplification test (NAAT) …
View article: Identification of<i>Streptococcus suis</i>Meningitis through Population-Based Surveillance, Togo, 2010–2014
Identification of<i>Streptococcus suis</i>Meningitis through Population-Based Surveillance, Togo, 2010–2014 Open
During 2010-2014, we enrolled 511 patients with suspected bacterial meningitis into surveillance in 2 districts of northern Togo. We identified 15 persons with Streptococcus suis infection; 10 had occupational contact with pigs, and 12 suf…
View article: Accurate detection of<i>Neisseria gonorrhoeae</i>ciprofloxacin susceptibility directly from genital and extragenital clinical samples: towards genotype-guided antimicrobial therapy
Accurate detection of<i>Neisseria gonorrhoeae</i>ciprofloxacin susceptibility directly from genital and extragenital clinical samples: towards genotype-guided antimicrobial therapy Open
Genotypic prediction of N. gonorrhoeae ciprofloxacin susceptibility directly from clinical samples was highly accurate and, in the absence of culture, will facilitate use of tailored therapy for gonococcal infection, sparing use of current…