Ken Maeda
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View article: Detection of alphacoronaviruses in Rhinolophus ferrumequinum in Ehime, Japan
Detection of alphacoronaviruses in Rhinolophus ferrumequinum in Ehime, Japan Open
Bats are suspected reservoirs for several emerging viruses, including coronaviruses. Here, we report the detection of coronaviruses in the insectivorous bat, Rhinolophus ferrumequinum, in Ehime Prefecture, located in Shikoku, the smallest …
View article: Crimean-Congo Hemorrhagic Fever Virus among Goats, Southern Bhutan
Crimean-Congo Hemorrhagic Fever Virus among Goats, Southern Bhutan Open
We conducted serosurveillance for Crimean-Congo hemorrhagic fever virus (CCHFV) among goats in southern Bhutan. Testing serum samples from 472 goats for CCHFV-specific IgG using an indirect fluorescent antibody test and ELISA, we found CCH…
View article: Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen
Bivalent single-domain antibodies show potent mpox virus neutralization through M1R antigen Open
Despite the recent mpox outbreak raising global concerns, no fully validated antiviral treatment exists, highlighting the urgent need for effective therapeutics. Here, by taking advantage of the preparation technology for single-domain (VH…
View article: Enhanced detection of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) antibodies in various animal species using oligomeric nucleocapsid protein-based diagnostics
Enhanced detection of Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) antibodies in various animal species using oligomeric nucleocapsid protein-based diagnostics Open
Severe Fever with Thrombocytopenia Syndrome Virus (SFTSV) is a zoonotic pathogen that affects various animal species, often asymptomatically, while causing fatal disease in humans. Current serological tests are limited, which led to the de…
View article: Genetically distinct hantaviruses in two bat species in Panamá
Genetically distinct hantaviruses in two bat species in Panamá Open
Recent discoveries of hantaviruses in bats in Europe, Asia, and Africa have prompted expanded explorations of their host diversity and geographic distribution. In screening lung tissue of 218 bats from Panamá, representing 19 genera and fi…
View article: Recent Hepatitis E Virus Infection in Wild Boars and Other Ungulates in Japan
Recent Hepatitis E Virus Infection in Wild Boars and Other Ungulates in Japan Open
Hepatitis E virus (HEV) is a zoonotic pathogen with multiple hosts, posing significant public health risks, especially in regions like Japan where game meat consumption is prevalent. This study investigated HEV infection and viral shedding…
View article: Application of versatile reverse genetics system for feline coronavirus
Application of versatile reverse genetics system for feline coronavirus Open
Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a sui…
View article: Genetic diversity and virulence of <i>Bacillus cereus</i> group isolates from bloodstream infections
Genetic diversity and virulence of <i>Bacillus cereus</i> group isolates from bloodstream infections Open
Bacillus cereus catheter-related bloodstream infections (CRBSIs) are an increasing concern in Japanese hospitals. Although their clinical characteristics have been explored, the genetic relationships and virulence profiles of B. cereus iso…
View article: Epidemiological Survey of Canine Distemper Virus Infection: Exploring the Link Between Virus Spread and Invasive Raccoon (<i>Procyon lotor</i>) Population Growth in Hokkaido, Japan
Epidemiological Survey of Canine Distemper Virus Infection: Exploring the Link Between Virus Spread and Invasive Raccoon (<i>Procyon lotor</i>) Population Growth in Hokkaido, Japan Open
Invasive raccoons ( Procyon lotor ) naturalized in Hokkaido, Japan, potentially spreading infectious diseases. Canine distemper virus infection is a serious epizootic disease, for which the raccoon is one of the hosts. We investigated the …
View article: Genetic, Phylogenetic, and Serological Analysis of a Getah Virus Strain Isolated from <i>Culex tritaeniorhynchus</i> Mosquitoes in Nagasaki, Japan in 2022
Genetic, Phylogenetic, and Serological Analysis of a Getah Virus Strain Isolated from <i>Culex tritaeniorhynchus</i> Mosquitoes in Nagasaki, Japan in 2022 Open
Getah virus (GETV), belonging to the genus Alphavirus within the family Togaviridae, is a mosquito-borne virus that causes fever, rash, edema in horses, fatalities, and pregnancy disorders in pigs. It has caused occasional outbreaks in hor…
View article: Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2
Improved efficacy of SARS-CoV-2 isolation from COVID-19 clinical specimens using VeroE6 cells overexpressing TMPRSS2 and human ACE2 Open
The cell culture-based isolation of novel coronavirus SARS-CoV-2 from clinical specimens obtained from patients with suspected COVID-19 is important not only for laboratory diagnosis but also for obtaining live virus to characterize emergi…
View article: First versatile reverse genetics system for feline coronavirus
First versatile reverse genetics system for feline coronavirus Open
Feline infectious peritonitis (FIP) is a fatal disease caused by feline coronavirus (FCoV). Although multiple gene mutations in FCoV likely account for FIP pathogenesis, molecular studies for FCoV have been limited due to the lack of a sui…
View article: Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr
Green Tea Catechin Epigallocatechin Gallate Inhibits Vegetative Cell Outgrowth and Expression of Beta-Lactamase Genes in Penicillin-Resistant Bacillus anthracis Strain PCr Open
The green tea catechin epigallocatechin gallate (EGCg) has antimicrobial effects on many bacteria. In this study, we investigated the inhibitory effects of EGCg on Bacillus anthracis spores and vegetative cells. The B. anthracis spores wer…
View article: An outbreak of SARS-CoV-2 omicron variant and deaths of three lions in a zoo
An outbreak of SARS-CoV-2 omicron variant and deaths of three lions in a zoo Open
There have been reports of the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from humans to various mammalian species. Some infected animals show clinical signs and may even die in rare cases. Outbreaks of SA…
View article: Surveillance of SARS-CoV-2 Infection in Rodent Populations in Tokyo, Japan
Surveillance of SARS-CoV-2 Infection in Rodent Populations in Tokyo, Japan Open
The persistence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) among wildlife populations poses a potential risk for the emergence of novel variants. Therefore, monitoring SARS-CoV-2 infections in animals is crucial. As ur…
View article: Construction of Vero cell-adapted rabies vaccine strain by five amino acid substitutions in HEP-Flury strain
Construction of Vero cell-adapted rabies vaccine strain by five amino acid substitutions in HEP-Flury strain Open
Rabies virus (RABV) causes fatal neurological disease. Pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) using inactivated-virus vaccines are the most effective measures to prevent rabies. In Japan, HEP-Flury, the viral s…
View article: Identification and epidemiological study of an uncultured flavivirus from ticks using viral metagenomics and pseudoinfectious viral particles
Identification and epidemiological study of an uncultured flavivirus from ticks using viral metagenomics and pseudoinfectious viral particles Open
During their blood-feeding process, ticks are known to transmit various viruses to vertebrates, including humans. Recent viral metagenomic analyses using next-generation sequencing (NGS) have revealed that blood-feeding arthropods like tic…
View article: Single Amino Acid Substitution in the Matrix Protein of Rabies Virus Is Associated with Neurovirulence in Mice
Single Amino Acid Substitution in the Matrix Protein of Rabies Virus Is Associated with Neurovirulence in Mice Open
Rabies is a fatal encephalitic infectious disease caused by the rabies virus (RABV). RABV is highly neurotropic and replicates in neuronal cell lines in vitro. The RABV fixed strain, HEP-Flury, was produced via passaging in primary chicken…
View article: Draft genome sequence of <i>Yersinia pseudotuberculosis</i> isolated from a wild rat in Japan
Draft genome sequence of <i>Yersinia pseudotuberculosis</i> isolated from a wild rat in Japan Open
We report a draft genome sequence of Yersinia pseudotuberculosis isolated from the spleen of a wild rat from Mikura-shima Island, Japan. The bacterium was identified as serotype O:4b using PCR-based O-genotyping. These genomic data provide…
View article: An octavalent dendrimer of multiple antigenic peptide with a property of pan-coronavirus IgM induction improved clinical signs of feline infectious peritonitis in cats
An octavalent dendrimer of multiple antigenic peptide with a property of pan-coronavirus IgM induction improved clinical signs of feline infectious peritonitis in cats Open
Feline infectious peritonitis (FIP) is a lethal disease caused by a pathogenic coronavirus, feline infectious peritonitis virus (FIPV), in cats. Effective vaccines have been unsuccessful due to the frequent mutation of FIPV and antibody-de…
View article: Lethal severe fever with thrombocytopenia syndrome virus infection causes systemic germinal centre failure and massive T cell apoptosis in cats
Lethal severe fever with thrombocytopenia syndrome virus infection causes systemic germinal centre failure and massive T cell apoptosis in cats Open
Introduction Severe fever with thrombocytopenia syndrome (SFTS) is a fatal viral disease characterized by high fever, thrombocytopenia, leukopenia, and multi-organ haemorrhage. Disruption of the humoral immune response and decreased lympho…
View article: Cross-Neutralization Activities of Antibodies against 18 Lyssavirus Glycoproteins
Cross-Neutralization Activities of Antibodies against 18 Lyssavirus Glycoproteins Open
Some lyssaviruses, including the rabies virus (RABV), cause lethal neurological symptoms in humans. However, the efficacy of commercial vaccines has only been evaluated against RABV. To assess cross-reactivity among lyssaviruses, including…
View article: <i>Macacine alphaherpesvirus</i> 1 (B Virus) Infection in Humans, Japan, 2019
<i>Macacine alphaherpesvirus</i> 1 (B Virus) Infection in Humans, Japan, 2019 Open
Two human patients with Macacine alphaherpesvirus 1 infection were identified in Japan in 2019. Both patients had worked at the same company, which had a macaque facility. The rhesus-genotype B virus genome was detected in cerebrospinal fl…
View article: Establishment of serological neutralizing tests using pseudotyped viruses for comprehensive detection of antibodies against all 18 lyssaviruses
Establishment of serological neutralizing tests using pseudotyped viruses for comprehensive detection of antibodies against all 18 lyssaviruses Open
Rabies is a fatal zoonotic, neurological disease caused by rabies lyssavirus (RABV) and other lyssaviruses. In this study, we established novel serological neutralizing tests (NT) based on vesicular stomatitis virus pseudotypes possessing …