Kendra K. Frederick
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View article: Spatially resolved DNP-assisted NMR illuminates the conformational ensemble of α-synuclein in intact viable cells
Spatially resolved DNP-assisted NMR illuminates the conformational ensemble of α-synuclein in intact viable cells Open
The protein α-syn adopts a wide variety of conformations including an intrinsically disordered monomeric form and an α-helical-rich membrane-associated form that is thought to play an important role in cellular membrane processes. However,…
View article: Stability of the polarization agent AsymPolPOK in intact and lysed mammalian cells
Stability of the polarization agent AsymPolPOK in intact and lysed mammalian cells Open
Dynamic nuclear polarization (DNP) solid-state NMR enables detection of proteins at endogenous concentrations in cells through sensitivity enhancement from nitroxide biradical polarization agents. AsymPolPOK, a novel water-soluble asymmetr…
View article: Structural context modulates the conformational ensemble of the intrinsically disordered amino terminus of α-synuclein
Structural context modulates the conformational ensemble of the intrinsically disordered amino terminus of α-synuclein Open
Regions of intrinsic disorder play crucial roles in biological systems, yet they often elude characterization by conventional biophysical techniques. To capture conformational distributions across different timescales, we employed a freezi…
View article: In cell NMR reveals cells selectively amplify and structurally remodel amyloid fibrils
In cell NMR reveals cells selectively amplify and structurally remodel amyloid fibrils Open
Amyloid forms of α-synuclein adopt different conformations depending on environmental conditions. Advances in structural biology have accelerated fibril characterization. However, it remains unclear which conformations predominate in biolo…
View article: Spatially resolved DNP-assisted NMR illuminates the conformational ensemble of α-synuclein in intact viable cells
Spatially resolved DNP-assisted NMR illuminates the conformational ensemble of α-synuclein in intact viable cells Open
The protein α-syn adopts a wide variety of conformations including an intrinsically disordered monomeric form and an α-helical rich membrane-associated form that is thought to play an important role in cellular membrane processes. However,…
View article: Conformational ensembles explain <scp>NMR</scp> spectra of frozen intrinsically disordered proteins
Conformational ensembles explain <span>NMR</span> spectra of frozen intrinsically disordered proteins Open
Protein regions which are intrinsically disordered, exist as an ensemble of rapidly interconverting structures. Cooling proteins to cryogenic temperatures for dynamic nuclear polarization (DNP) magic angle spinning (MAS) NMR studies suspen…
View article: DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular environments
DNP-assisted solid-state NMR enables detection of proteins at nanomolar concentrations in fully protonated cellular environments Open
With the sensitivity enhancements conferred by dynamic nuclear polarization (DNP), magic angle spinning (MAS) solid state NMR spectroscopy experiments can attain the necessary sensitivity to detect very low concentrations of proteins. This…
View article: The conformational ensemble of an intrinsically disordered protein explains peak shapes under DNP conditions
The conformational ensemble of an intrinsically disordered protein explains peak shapes under DNP conditions Open
Elucidating the conformational preferences of regions of intrinsic disorder in biologically relevant contexts represents a frontier of structural biology. The sensitivity enhancements conferred by DNP enable structural studies of proteins …
View article: In-Cell NMR of Intact Mammalian Cells Preserved with the Cryoprotectants DMSO and Glycerol Have Similar DNP Performance
In-Cell NMR of Intact Mammalian Cells Preserved with the Cryoprotectants DMSO and Glycerol Have Similar DNP Performance Open
NMR has the resolution and specificity to determine atomic-level protein structures of isotopically-labeled proteins in complex environments and, with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensi…
View article: In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Viable Mammalian Cells Open
NMR has the resolution and specificity to determine atomic-level protein structures of isotopically labeled proteins in complex environments, and with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensi…
View article: In-Cell Sensitivity-Enhanced NMR of Intact Living Mammalian Cells
In-Cell Sensitivity-Enhanced NMR of Intact Living Mammalian Cells Open
NMR has the resolution and specificity to determine atomic-level protein structures of isotopically-labeled proteins in complex environments and, with the sensitivity gains conferred by dynamic nuclear polarization (DNP), NMR has the sensi…
View article: Michler’s hydrol blue elucidates structural differences in prion strains
Michler’s hydrol blue elucidates structural differences in prion strains Open
Significance Amyloids are protein fiber aggregates that are associated with both pathology, e.g., neurodegenerative diseases, and functionality, e.g., adaptation to environmental changes in yeasts. A particular property of amyloid fibrils …
View article: Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability Open
Dynamic nuclear polarization (DNP) can dramatically increase the sensitivity of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy. These sensitivity gains increase as temperatures decrease and are large enough to ena…
View article: Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability Open
Dynamic nuclear polarization (DNP) can dramatically increase the sensitivity of magic angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy. These sensitivity gains increase as temperatures decrease and are large enough to ena…
View article: Amyloid fibrils embodying distinctive yeast prion phenotypes exhibit diverse morphologies
Amyloid fibrils embodying distinctive yeast prion phenotypes exhibit diverse morphologies Open
Yeast prions are self-templating protein-based mechanisms of inheritance whose conformational changes lead to the acquisition of diverse new phenotypes. The best studied of these is the prion domain (NM) of Sup35, which forms an amyloid th…
View article: Aggregation and Fibril Structure of Aβ<sub>M01–42</sub> and Aβ<sub>1–42</sub>
Aggregation and Fibril Structure of Aβ<sub>M01–42</sub> and Aβ<sub>1–42</sub> Open
A mechanistic understanding of Aβ aggregation and high-resolution structures of Aβ fibrils and oligomers are vital to elucidating relevant details of neurodegeneration in Alzheimer's disease, which will facilitate the rational design of di…
View article: Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register
Combining DNP NMR with segmental and specific labeling to study a yeast prion protein strain that is not parallel in-register Open
Significance Self-propagating changes in the conformation of amyloidogenic proteins play vital roles in normal biology and disease. Despite intense research, the architecture of amyloid fibers remains poorly understood. In this work, we us…