Kenichiro Sakaguchi
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View article: Effects of heat stress on gene expression associated with antioxidant capacity of bovine early antral follicles
Effects of heat stress on gene expression associated with antioxidant capacity of bovine early antral follicles Open
Heat exposure at the physiological level of oocyte-cumulus-granulosa cell complexes (OCGCs) during in vitro growth (IVG) culture decreases the reduced glutathione (GSH) level of oocytes, an antioxidant, but its mechanism has been unclear. …
View article: Optimization of ovum pick-up-<i>in vitro</i> fertilization and <i>in vitro</i> growth of immature oocytes in ruminants
Optimization of ovum pick-up-<i>in vitro</i> fertilization and <i>in vitro</i> growth of immature oocytes in ruminants Open
Due to the strong demand for embryo production from young and genotyped superior animals using ovum-pick up (OPU) combined with in vitro fertilization (IVF), the number of in vitro-produced embryos has exceeded that of in vivo-derived embr…
View article: In Vitro Growth of Mammalian Follicles and Oocytes
In Vitro Growth of Mammalian Follicles and Oocytes Open
Mammalian ovaries contain a large number of immature follicles, most of which are destined to degenerate before ovulation [...]
View article: Physiological high temperatures alter the amino acid metabolism of bovine early antral follicles
Physiological high temperatures alter the amino acid metabolism of bovine early antral follicles Open
Heat stress reduces the developmental competence of bovine oocytes during the growth phase; however, the detailed mechanisms remain unclear. Amino acids play various critical roles in follicular development, including protein synthesis and…
View article: SEMI-QUANTITATIVE EVALUATION OF MASSETER MUSCLE STIFFNESS IN PATIENTS WITH TEMPOROMANDIBULAR DISORDERS USING STRAIN ELASTOGRAPHY
SEMI-QUANTITATIVE EVALUATION OF MASSETER MUSCLE STIFFNESS IN PATIENTS WITH TEMPOROMANDIBULAR DISORDERS USING STRAIN ELASTOGRAPHY Open
View article: Relationship between Amino Acid Metabolism and Bovine In Vitro Follicle Activation and Growth
Relationship between Amino Acid Metabolism and Bovine In Vitro Follicle Activation and Growth Open
The amino acid metabolism of bovine follicles during in vitro growth (IVG) was evaluated to identify potential indicators of health during culture. The bovine ovarian cortex was sliced, prepared as strips, and cultured for 6 days. Tissue s…
View article: Effect of heat exposure on the growth and developmental competence of bovine oocytes derived from early antral follicles
Effect of heat exposure on the growth and developmental competence of bovine oocytes derived from early antral follicles Open
In dairy cows, low fertility caused by summer heat stress continues into the cooler autumn season. This can be caused by impaired oocyte quality in small growing follicles during summer. Here, we subjected oocyte–cumulus–granulosa complexe…
View article: Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels
Blastocyst formation, embryo transfer and breed comparison in the first reported large scale cloning of camels Open
Cloning, through somatic cell nuclear transfer (SCNT), has the potential for a large expansion of genetically favorable traits in a population in a relatively short term. In the present study we aimed to produce multiple cloned camels from…
View article: Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes
Development and pregnancy rates of Camelus dromedarius-cloned embryos derived from in vivo- and in vitro-matured oocytes Open
Objective: The present study evaluated the efficiency of embryo development and pregnancy of somatic cell nuclear transfer (SCNT) embryos using different source-matured oocytes in Camelus dromedarius.Methods: Camelus dromedarius embryos we…
View article: Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro
Follicle priming by FSH and pre-maturation culture to improve oocyte quality in vivo and in vitro Open
View article: Relationship between the timing of the first postpartum ovulation and antral follicle counts in Holstein cows
Relationship between the timing of the first postpartum ovulation and antral follicle counts in Holstein cows Open
Background The timing of the first postpartum ovulation is an important factor affecting the timing of estrous resumption in dairy cows. The first postpartum ovulation is delayed in cows producing large amounts of milk with an intensive ne…
View article: Relationships between the antral follicle count, steroidogenesis, and secretion of follicle-stimulating hormone and anti-Müllerian hormone during follicular growth in cattle
Relationships between the antral follicle count, steroidogenesis, and secretion of follicle-stimulating hormone and anti-Müllerian hormone during follicular growth in cattle Open
View article: Effects of follicle‐stimulating hormone followed by gonadotropin‐releasing hormone on embryo production by ovum pick‐up and in vitro fertilization in the river buffalo (<i>Bubalus bubalis</i>)
Effects of follicle‐stimulating hormone followed by gonadotropin‐releasing hormone on embryo production by ovum pick‐up and in vitro fertilization in the river buffalo (<i>Bubalus bubalis</i>) Open
In this study, we examined the effects of superstimulation using follicle‐stimulating hormone ( FSH ) followed by gonadotropin‐releasing hormone (Gn RH ) on buffalo embryo production by ultrasound‐guided ovum pick‐up ( OPU ) and in vitro f…
View article: Effect of astaxanthin addition to an individual culture system for in vitro maturation of bovine oocytes on accumulation of reactive oxygen species and mitochondrial activity
Effect of astaxanthin addition to an individual culture system for in vitro maturation of bovine oocytes on accumulation of reactive oxygen species and mitochondrial activity Open
View article: Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes
Relationship between the antral follicle count in bovine ovaries from a local abattoir and steroidogenesis of granulosa cells cultured as oocyte-cumulus-granulosa complexes Open
The antral follicle count (AFC) is used as an indicator of cow fertility. We herein investigated the relationship between AFC and the steroidogenesis of granulosa cells and confirmed the developmental competence of oocytes derived from ear…
View article: Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes
Effects of pre-maturational culture duration on developmental competence of bovine small-sized oocytes Open
We investigated the effects of pre-maturational (pre-IVM) culture on the developmental competence of small-sized bovine oocytes (110 and < 115 µm). Oocytes were cultured with 3-isobutyl-1-methylxanthine (IBMX) for 0, 5, or 10 h and subject…
View article: Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for <i>in vivo</i> and <i>in vitro</i> embryo production in Japanese black cows
Effect of a single epidural administration of follicle-stimulating hormone via caudal vertebrae on superstimulation for <i>in vivo</i> and <i>in vitro</i> embryo production in Japanese black cows Open
Here, we describe a simplified procedure for embryo production in the Japanese black cow that uses a single caudal epidural injection of follicle-stimulating hormone (FSH). First, we compared the efficiency of superovulation for in vivo em…
View article: Relationship between in vitro growth of bovine oocytes and steroidogenesis of granulosa cells cultured in medium supplemented with bone morphogenetic protein-4 and follicle stimulating hormone
Relationship between in vitro growth of bovine oocytes and steroidogenesis of granulosa cells cultured in medium supplemented with bone morphogenetic protein-4 and follicle stimulating hormone Open