Explanipedia

643. Development of Anionic Nanocomplexes for Targeted SiRNA and Plasmid DNA Delivery Open

AD Tagalakis, G E Kenny, Luísa Saraiva, David McCarthy, Stephen L. Hart · 2012

Susceptibilities of Mycoplasma pneumoniae, Mycoplasma hominis, and Ureaplasma urealyticum to a new quinolone, trovafloxacin (CP-99,219) Open

G E Kenny, F D Cartwright · 1996

The susceptibilities of mycoplasmas to a new quinolone, trovafloxacin (CP-99,219), were compared with those to sparfloxacin and ofloxacin. Mycoplasma pneumoniae was as susceptible to trovafloxacin (MIC = 0.25 microgram/ml) as to sparfloxac…

Susceptibilities of Mycoplasma hominis and Ureaplasma urealyticum to two new quinolones, sparfloxacin and WIN 57273 Open

G E Kenny, F D Cartwright · 1991

Mycoplasma hominis was highly susceptible to two new quinolones, with MICs for 90% of isolates tested of 0.004 micrograms/ml for WIN 57273 and 0.063 micrograms/ml for sparfloxacin, which were activities much greater than the 1 microgram/ml…

Identification of a mycoplasmal protein which binds immunoglobulins nonimmunologically Open

A G Alexander, H R Lowes, G E Kenny · 1991

Immunoblotted protein samples from several strains of Mycoplasma hominis and from one strain of Mycoplasma arginini each contain a polypeptide of a molecular mass of 95,000 to 105,000 Da which binds immunoglobulin nonimmunologically. Immun…

Virulence of ureaplasmal urease for mice Open

J V Ligon, G E Kenny · 1991

Ureaplasmas killed mice within 5 min after intravenous injection. The 50% lethal dose of whole ureaplasmal organisms was 32 micrograms per mouse, a value also found for crystalline jackbean urease. The reaction was specific to urease, sinc…

Susceptibility of Mycoplasma pneumoniae to several new quinolones, tetracycline, and erythromycin Open

G E Kenny, F D Cartwright · 1991

Mycoplasma pneumoniae (39 strains) was most susceptible to two quinolones, WIN 57273 and sparfloxacin, with MICs for 90% of the strains (MIC90S) of 0.125 and 0.25 micrograms/ml, respectively. It was susceptible to ofloxacin and ciprofloxac…

Quantitation of antibody reactivity to human immunodeficiency virus (type 1) proteins and glycoproteins on Western immunoblots by reflectance densitometry Open

C T Hardy, Todd Damrow, G E Kenny · 1990

The human serum antibody response to polypeptides of human immunodeficiency virus type 1 (HIV-1) was quantitated by reflectance densitometry of Western immunoblots by using two commercially available blotting systems. In one system, human …

Diagnosis of Mycoplasma pneumoniae pneumonia: sensitivities and specificities of serology with lipid antigen and isolation of the organism on soy peptone medium for identification of infections Open

G E Kenny, GLADYS KAISER, Marion K. Cooney, H. M. Foy · 1990

The sensitivities and specificities of isolation and serology for detection of Mycoplasma pneumoniae infections were determined for 3,546 pneumonia patients for whom both isolation and serological data were available. Soy peptone, fresh ye…

Susceptibilities of genital mycoplasmas to the newer quinolones as determined by the agar dilution method Open

G E Kenny, Thomas M. Hooton, Marilyn C. Roberts, F D Cartwright, Jill M. Hoyt · 1989

The increasing resistance of genital mycoplasmas to tetracycline poses a problem because tetracycline is one of the few antimicrobial agents active against Mycoplasma hominis, Ureaplasma urealyticum, chlamydiae, gonococci, and other agents…

Characteristics of Ureaplasma urealyticum urease Open

Alain Blanchard, Sergey V. Razin, G E Kenny, Michael F. Barile · 1988

Sonication of Ureaplasma urealyticum cells grown in a dialysate growth medium effectively separated the cytoplasmic fraction from the membrane fraction, with both fractions relatively free from exogenous contaminating proteins. The urease …

Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe Open

David Yogev, D. Halachmi, G E Kenny, Sergey V. Razin · 1988

Genomic fingerprints of Acholeplasma laidlawii, Mycoplasma hominis, and Mycoplasma pneumoniae strains were obtained by Southern blot hybridization of the digested mycoplasmal DNAs with an rRNA gene probe. The hybridization patterns reveale…

Antigenic differences in the surface mannoproteins of Candida albicans as revealed by monoclonal antibodies Open

Paula Sundstrom, Milton R. Tam, E J Nichols, G E Kenny · 1988

Monoclonal antibodies to Candida albicans were prepared with blastoconidia bearing germ tubes used as the immunogen. Four antibodies reacted by immunofluorescence with surfaces of C. albicans as well as Candida stellatoidea, Candida tropic…

Humoral immune response to polypeptides of Ureaplasma urealyticum in women with postpartum fever Open

Gyesoon Yoon Lee, G E Kenny · 1987

We investigated the antibody response in women with postpartum fever from whom Ureaplasma urealyticum had been isolated from the bloodstream. Acute- and convalescent-phase sera were tested for immunoglobulin G to the polypeptides of five s…

The principal protein antigens of isolates of Mycoplasma pneumoniae as measured by levels of immunoglobulin G in human serum are stable in strains collected over a 10-year period Open

Alyssa Vu, H. M. Foy, F D Cartwright, G E Kenny · 1987

To determine whether antigenic variation in protein antigens of Mycoplasma pneumoniae occurred over time, 12 isolates obtained from pneumonia patients over a 10-year period (1964 to 1974) were compared by immunoblotting (Western blotting) …

Conjugal transfer of transposon Tn916 from Streptococcus faecalis to Mycoplasma hominis Open

Marilyn C. Roberts, G E Kenny · 1987

Transposon Tn916 was transferred from Streptococcus faecalis to Mycoplasma hominis by a mating process resembling conjugation with a frequency of 10(-6) to 10(-7). Tn916 was inserted into the mycoplasmal chromosome in single and multiple c…

Chromosomal DNA Probes for the Identification of Bacteroides Species Open

Marilyn C. Roberts, Bernard J. Moncla, G E Kenny · 1987

We compared 22 Bacteroides species by DNA-DNA homology studies using the S1 endonuclease method. None of the currently defined species shared more than 30% DNA homology with any other species examined with the exception of B. buccae and B.…

Antigenic differences between mannoproteins of germ tubes and blastospores of Candida albicans Open

Paula Sundstrom, E J Nichols, G E Kenny · 1987

To determine the nature of germ tube-specific antigens of Candida albicans, procedures for intrinsically labeling cell wall antigens metabolically were developed. Blastospores or germ tubes labeled either in their proteins with L-[35S]meth…

Tetracycline resistance and tetM in pathogenic urogenital bacteria Open

Marilyn C. Roberts, Sharon L. Hillier, J Hale, King K. Holmes, G E Kenny · 1986

Clinical isolates of Gardnerella vaginalis, Streptococcus agalactiae, Bacteroides spp., and Mobiluncus spp. were screened for resistance to tetracycline and for the presence of the streptococcal tetM determinant. The S. agalactiae and G. v…

Dissemination of the tetM tetracycline resistance determinant to Ureaplasma urealyticum Open

Marilyn C. Roberts, G E Kenny · 1986

Ureaplasma urealyticum is an organism considered susceptible to tetracycline. Ten tetracycline-resistant (Tcr) clinical isolates and Tcr serotype 9 were examined. All contained DNA sequences homologous to the streptococcal determinant tetM…

Enzymatic release of germ tube-specific antigens from cell walls of Candida albicans Open

Paula Sundstrom, G E Kenny · 1985

The differences between blastospores and germ tubes of Candida albicans, as previously shown by immunofluorescence, were further studied by comparing digests of cell walls of both growth forms. Organisms were surface labeled with 125I, and…

Tetracycline-resistant Mycoplasma hominis strains contain streptococcal tetM sequences Open

Marilyn C. Roberts, Laura A. Koutsky, King K. Holmes, Donald J. LeBlanc, G E Kenny · 1985

Clinical isolates of Mycoplasma hominis resistant to high levels of tetracycline contained DNA sequences homologous to the streptococcal tetracycline determinant, tetM. In contrast, none of the susceptible M. hominis isolates tested carrie…

Antigenic distinctiveness of Mobiluncus curtisii and Mobiluncus mulieris Open

Marilyn C. Roberts, E. J. Baron, S M Finegold, G E Kenny · 1985

A total of 26 Mobiluncus strains (17 M. curtisii and 9 M. mulieris strains) were compared serologically by double immunodiffusion and immunoblotting against antisera prepared against representative isolates of each species. All strains fro…

Haemophilus influenzae type b isolates show antigenic variation in a major outer membrane protein Open

Alice L. Erwin, G E Kenny · 1984

Antigenic variation of the outer membrane proteins among isolates of Haemophilus influenzae was examined by immunoblotting. Rabbit antisera were raised against six strains of H. influenzae type b and tested against outer membrane preparati…

Immunochemical characterization of a heat-stable surface antigen of Mycoplasma pulmonis expressing both species-specific and strain-specific determinants Open

E J Nichols, G E Kenny · 1984

We sought to characterize the strain-specific antigens of four strains of Mycoplasma pulmonis (47, 63, Negroni, and 19612) by crossed immunoelectrophoresis. Although the strains possessed a number of common antigens, type-specific antigens…

Characterization of antigens specific to the surface of germ tubes of Candida albicans by immunofluorescence Open

Paula Sundstrom, G E Kenny · 1984

To characterize germ tube-specific antigens of Candida albicans, rabbit antiserum prepared to Formalin-treated yeast possessing germ tubes was adsorbed with stationary-phase blastospores. By immunofluorescence and enzyme-linked immunosorbe…

Identification and preliminary characterization of a major heat-stable surface antigen of Actinomyces israelii by two-dimensional (crossed) immunoelectrophoresis Open

G Y Ayakawa, Betsy L. Williams, G E Kenny · 1983

We have identified, employing two-dimensional (crossed) immunoelectrophoresis, a major heat-stable surface antigen in Actinomyces israelii with an electrophoretic mobility of 0.86 compared to bovine albumin. The surface component was prote…

Principles, problems, and strategies in the use of antigenic mixtures for the enzyme-linked immunosorbent assay Open

G E Kenny, Carol L. Dunsmoor · 1983

Competition between proteins and other macromolecules for adsorption sites on plastic was studied with the enzyme-linked immunosorbent assay (ELISA) to determine effects of the use of antigenic mixtures or extracts of organisms on assays o…

Broadly reactive immunofluorescence test for measurement of immunoglobulin M and G antibodies to Ureaplasma urealyticum in infant and adult sera Open

D Gallo, Kent Dupuis, Nathalie J. Schmidt, G E Kenny · 1983

The indirect immunofluorescence test was used to measure immunoglobulin M (IgM) and IgG antibodies to acetone-fixed Ureaplasma urealyticum organisms in sera from 128 adults with genital infections and from 713 symptomatic newborns and babi…

Rapid method for simultaneous detection of the arginine dihydrolase system and amino acid decarboxylases in microorganisms Open

Keyao Chen, N J Culbertson, J S Knapp, G E Kenny, King K. Holmes · 1982

A specific procedure has been developed for the detection of the first two enzymes involved in the arginine dihydrolase system and the detection of the decarboxylases of arginine, glutamic acid, histidine, lysine, ornithine, phenylalanine,…

Antigenic groupings of 90 rhinovirus serotypes Open

Marion K. Cooney, J. P. Fox, G E Kenny · 1982

We have completed production in rabbits of potent antisera to the 90 classified rhinovirus serotypes by using methods previously described (M. K. Cooney and G. E. Kenny, Proc. Soc. Exp. Biol. Med. 133:645-650, 1970). Systematic testing by …

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