Kevin K. Tsia
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View article: Generalizable morphological profiling of cells by interpretable unsupervised learning
Generalizable morphological profiling of cells by interpretable unsupervised learning Open
The intersection of advanced microscopy and machine learning is transforming cell biology into a quantitative, data-driven field. Traditional cell profiling depends on manual feature extraction, which is labor-intensive and prone to bias, …
View article: FACED 2.0 enables large-scale voltage and calcium imaging in vivo
FACED 2.0 enables large-scale voltage and calcium imaging in vivo Open
Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. Here we optimized a kilohertz-frame-rate two-photon fluorescence microscope with an all-op…
View article: On-The-Fly Live-Cell Intrinsic Morphological Drug and Genetic Screens by Gigapixel-per-second Spinning Arrayed Disk Imaging
On-The-Fly Live-Cell Intrinsic Morphological Drug and Genetic Screens by Gigapixel-per-second Spinning Arrayed Disk Imaging Open
Next-generation drug discovery and functional genomics require rapid, unbiased single-cell profiling at scale—demands that exceed the limited speed, throughput, and labor-intensive labeling constraints of conventional high-content image-ba…
View article: Multiplane compressive imaging with axial-coded multiphoton microscopy
Multiplane compressive imaging with axial-coded multiphoton microscopy Open
View article: FACED 2.0 enables large-scale voltage and calcium imaging <i>in vivo</i>
FACED 2.0 enables large-scale voltage and calcium imaging <i>in vivo</i> Open
Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. We optimized a kilohertz-frame-rate two-photon fluorescence microscope with all-optical me…
View article: Intelligent soliton molecules control in an ultrafast thulium fiber laser
Intelligent soliton molecules control in an ultrafast thulium fiber laser Open
View article: StaVia: spatially and temporally aware cartography with higher-order random walks for cell atlases
StaVia: spatially and temporally aware cartography with higher-order random walks for cell atlases Open
View article: Hybrid optical parametrically oscillating emitter-enabled photoacoustic imaging of water: enhanced contrast, dynamic range, and multifaceted applications
Hybrid optical parametrically oscillating emitter-enabled photoacoustic imaging of water: enhanced contrast, dynamic range, and multifaceted applications Open
View article: Information‐Distilled Generative Label‐Free Morphological Profiling Encodes Cellular Heterogeneity
Information‐Distilled Generative Label‐Free Morphological Profiling Encodes Cellular Heterogeneity Open
Image‐based cytometry faces challenges due to technical variations arising from different experimental batches and conditions, such as differences in instrument configurations or image acquisition protocols, impeding genuine biological int…
View article: Front Matter: Volume 12853
Front Matter: Volume 12853 Open
View article: StaVia: Spatially and temporally aware cartography with higher order random walks for cell atlases
StaVia: Spatially and temporally aware cartography with higher order random walks for cell atlases Open
Single-cell atlases are critical for unraveling the cellular basis of health and disease, yet their sheer diversity and vast data landscape in time and space pose daunting computational challenges pertaining to the delineation of multiple …
View article: Dispersion-free inertial focusing (DIF) for high-yield polydisperse micro-particles filtration and analysis
Dispersion-free inertial focusing (DIF) for high-yield polydisperse micro-particles filtration and analysis Open
Inertial focusing excels at the precise spatial ordering and separation of microparticles by size within fluid flows. However, this advantage, brought by its inherent size-dependent dispersion, could turn into a drawback that challenges ap…
View article: Information-Distilled Generative Label-Free Morphological Profiling Encodes Cellular Heterogeneity
Information-Distilled Generative Label-Free Morphological Profiling Encodes Cellular Heterogeneity Open
Image-based cytometry faces constant challenges due to technical variations arising from different experimental batches and conditions, such as differences in instrument configurations or image acquisition protocols, impeding genuine biolo…
View article: Morphological profiling by high-throughput single-cell biophysical fractometry
Morphological profiling by high-throughput single-cell biophysical fractometry Open
View article: StarmapVis: An interactive and narrative visualisation tool for single-cell and spatial data
StarmapVis: An interactive and narrative visualisation tool for single-cell and spatial data Open
Current single-cell visualisation techniques project high dimensional data into 'map' views to identify high-level structures such as cell clusters and trajectories. New tools are needed to allow the transversal through the high dimensiona…
View article: EuniceScope: Low-Cost Imaging Platform for Studying Microgravity Cell Biology
EuniceScope: Low-Cost Imaging Platform for Studying Microgravity Cell Biology Open
Microgravity is proven to impact a wide range of human physiology, from stimulating stem cell differentiation to confounding cell health in bones, skeletal muscles, and blood cells. The research in this arena is progressively intensified b…
View article: Ultrafast optical phase-sensitive ultrasonic detection via dual-comb multiheterodyne interferometry
Ultrafast optical phase-sensitive ultrasonic detection via dual-comb multiheterodyne interferometry Open
Highly sensitive and broadband ultrasound detection is important for photoacoustic imaging, biomedical ultrasound, and ultrasonic nondestructive testing. The elasto-optical refractive index modulation induced by ultrasound arouses a trans…
View article: Deep-tissue two-photon microscopy with a frequency-doubled all-fiber mode-locked laser at 937 nm
Deep-tissue two-photon microscopy with a frequency-doubled all-fiber mode-locked laser at 937 nm Open
In two-photon microscopy, low illumination powers on samples and a high signal-to-noise ratio (SNR) of the excitation laser are highly desired for alleviating the problems of photobleaching and phototoxicity, as well as providing clean bac…
View article: Hysteresis and balance of backaction force on dielectric particles photothermally mediated by photonic nanojet
Hysteresis and balance of backaction force on dielectric particles photothermally mediated by photonic nanojet Open
Reversible control over the microparticle motion using light excites interesting applications in optofluidics, microswimmers, artificial optical matter, and biomedical engineering. The dielectric microspheres swim towards the near infrared…
View article: Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo
Ultrafast two-photon fluorescence imaging of cerebral blood circulation in the mouse brain in vivo Open
Significance Characterizing blood flow by tracking individual red blood cells as they move through vessels is essential for understanding vascular function. With high spatial resolution, two-photon fluorescence microscopy is the method of …
View article: Morphological profiling by high-throughput single-cell biophysical fractometry
Morphological profiling by high-throughput single-cell biophysical fractometry Open
Complex and irregular cell architecture is known to statistically exhibit fractal geometry, i.e., a pattern resembles a smaller part of itself. Although fractal variations in cells are proven to be closely associated with the disease-relat…
View article: Tomographic-encoded multiphoton (TEMP) microscopy
Tomographic-encoded multiphoton (TEMP) microscopy Open
Axial scanning in multiphoton microscopy (MPM) is typically realized by mechanically shifting either the objective or the sample. However, the scan speed is usually hindered by the mechanical inertia of the bulky mass. Although the extende…
View article: Publisher Correction: Speed scaling in multiphoton fluorescence microscopy
Publisher Correction: Speed scaling in multiphoton fluorescence microscopy Open
View article: Generalized and scalable trajectory inference in single-cell omics data with VIA
Generalized and scalable trajectory inference in single-cell omics data with VIA Open
View article: Multimodal FACED imaging for large-scale single-cell morphological profiling
Multimodal FACED imaging for large-scale single-cell morphological profiling Open
Free-space angular-chirp-enhanced delay (FACED) is an ultrafast laser-scanning technique that allows for high imaging speed at the scale orders of magnitude greater than the current technologies. However, this speed advantage has only been…
View article: Optical volumetric brain imaging: speed, depth, and resolution enhancement
Optical volumetric brain imaging: speed, depth, and resolution enhancement Open
Understanding how the brain functions is one of the grand challenges in modern scientific research. Similar to a computer, a functional brain is composed of hardware and software. The major bottleneck lies in the difficulty to directly obs…
View article: Front Matter: Volume 11654
Front Matter: Volume 11654 Open
View article: FP12.04 Intelligent Label-Free Image-Based Profiling for Lung Cancer Cell Detection and Classification
FP12.04 Intelligent Label-Free Image-Based Profiling for Lung Cancer Cell Detection and Classification Open
View article: VIA: Generalized and scalable trajectory inference in single-cell omics data
VIA: Generalized and scalable trajectory inference in single-cell omics data Open
Inferring cellular trajectories using a variety of omic data is a critical task in single-cell data science. However, accurate prediction of cell fates, and thereby biologically meaningful discovery, is challenged by the sheer size of sing…
View article: Low-Latency <i>In Situ</i> Image Analytics With FPGA-Based Quantized Convolutional Neural Network
Low-Latency <i>In Situ</i> Image Analytics With FPGA-Based Quantized Convolutional Neural Network Open
Real-time in situ image analytics impose stringent latency requirements on intelligent neural network inference operations. While conventional software-based implementations on the graphic processing unit (GPU)-accelerated platforms are fl…