Kevin Prause
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View article: Live‐Cell Identification of Inhibitors of the Lipid Transfer Protein CERT Using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET)
Live‐Cell Identification of Inhibitors of the Lipid Transfer Protein CERT Using Nanoluciferase Bioluminescence Resonance Energy Transfer (NanoBRET) Open
A BRET system is described, in which Nanoluciferase was fused to the lipid transfer protein CERT for efficient energy transfer to a Nile red‐labeled ceramide, which is either directly bound to CERT or transported to the adjacent Golgi memb…
View article: Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris Open
The non-conventional yeast Pichia pastoris (syn. Komagataella phaffii ) has become a powerful eukaryotic expression platform for biopharmaceutical and biotechnological applications on both laboratory and industrial scales. Despite the fund…
View article: A photocaged inhibitor of acid sphingomyelinase
A photocaged inhibitor of acid sphingomyelinase Open
An esterase-labile, photocaged inhibitor provides spatiotemporal control over acid sphingomyelinase in living cells.
View article: Sphingolipid-Transporting Proteins as Cancer Therapeutic Targets
Sphingolipid-Transporting Proteins as Cancer Therapeutic Targets Open
The understanding of the role of sphingolipid metabolism in cancer has tremendously increased in the past ten years. Many tumors are characterized by imbalances in sphingolipid metabolism. In many cases, disorders of sphingolipid metabolis…