Keyi Geng
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View article: Intrinsic deletion at 10q23.31, including the <i>PTEN</i> gene locus, is aggravated upon CRISPR-Cas9–mediated genome engineering in HAP1 cells mimicking cancer profiles
Intrinsic deletion at 10q23.31, including the <i>PTEN</i> gene locus, is aggravated upon CRISPR-Cas9–mediated genome engineering in HAP1 cells mimicking cancer profiles Open
The CRISPR-Cas9 system is a powerful tool for studying gene functions and holds potential for disease treatment. However, precise genome editing requires thorough assessments to minimize unintended on- and off-target effects. Here, we repo…
View article: Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription
Coinhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer via readthrough transcription Open
Pancreatic carcinoma lacks effective therapeutic strategies resulting in poor prognosis. Transcriptional dysregulation due to alterations in KRAS and MYC affects initiation, development, and survival of this tumor type. Using patient-deriv…
View article: Estrogen receptor activation remodels<i>TEAD1</i>gene expression to alleviate nonalcoholic fatty liver disease
Estrogen receptor activation remodels<i>TEAD1</i>gene expression to alleviate nonalcoholic fatty liver disease Open
Introduction The occurrence of obesity-related hepatic malignancies differs between sexes, suggesting the involvement of sex hormones. Female sex hormones maintain cell homeostasis through estrogen receptor (ER) signaling and protect from …
View article: CRISPR-Cas9-mediated genome engineering exaggerates genomic deletion at 10q23.31 including the<i>PTEN</i>gene locus mimicking cancer profiles
CRISPR-Cas9-mediated genome engineering exaggerates genomic deletion at 10q23.31 including the<i>PTEN</i>gene locus mimicking cancer profiles Open
The CRISPR-Cas9 system is a powerful tool for studying gene functions and has tremendous potential for disease treatment. However, precise genome editing requires thorough assessments to minimize unintended on- and off-target effects. Here…
View article: Co-inhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer<i>via</i>readthrough transcription
Co-inhibition of topoisomerase 1 and BRD4-mediated pause release selectively kills pancreatic cancer<i>via</i>readthrough transcription Open
Pancreatic carcinoma is one of the most lethal cancers and the absence of efficient therapeutic strategies results in poor prognosis. Transcriptional dysregulation due to alterations in KRAS and MYC impacts initiation, development, and sur…
View article: Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells
Target-enriched nanopore sequencing and de novo assembly reveals co-occurrences of complex on-target genomic rearrangements induced by CRISPR-Cas9 in human cells Open
The CRISPR-Cas9 system is widely used to permanently delete genomic regions via dual guide RNAs. Genomic rearrangements induced by CRISPR-Cas9 can occur, but continuous technical developments make it possible to characterize complex on-tar…
View article: CCT3- <i>LINC00326</i> axis regulates hepatocarcinogenic lipid metabolism
CCT3- <i>LINC00326</i> axis regulates hepatocarcinogenic lipid metabolism Open
Objective To better comprehend transcriptional phenotypes of cancer cells, we globally characterised RNA-binding proteins (RBPs) to identify altered RNAs, including long non-coding RNAs (lncRNAs). Design To unravel RBP-lncRNA interactions …
View article: Nanoengineered DNA origami with repurposed TOP1 inhibitors targeting myeloid cells for the mitigation of neuroinflammation
Nanoengineered DNA origami with repurposed TOP1 inhibitors targeting myeloid cells for the mitigation of neuroinflammation Open
Targeting myeloid cells, especially microglia, for the treatment of neuroinflammatory diseases such as multiple sclerosis (MS), is underappreciated. Here, we screened a library of compounds and identified the topoisomerase 1 (TOP1) inhibit…
View article: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells
CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells Open
The CRISPR/Cas9 system is widely used to permanently delete genomic regions by inducing double-strand breaks via dual guide RNAs. However, on-target consequences of Cas9 deletion events have yet to be fully investigated. To characterize Ca…
View article: Genome Size Reduction and Transposon Activity Impact tRNA Gene Diversity While Ensuring Translational Stability in Birds
Genome Size Reduction and Transposon Activity Impact tRNA Gene Diversity While Ensuring Translational Stability in Birds Open
As a highly diverse vertebrate class, bird species have adapted to various ecological systems. How this phenotypic diversity can be explained genetically is intensively debated and is likely grounded in differences in the genome content. L…
View article: Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids
Successful delivery of large-size CRISPR/Cas9 vectors in hard-to-transfect human cells using small plasmids Open
With the rise of new powerful genome engineering technologies, such as CRISPR/Cas9, cell models can be engineered effectively to accelerate basic and disease research. The most critical step in this procedure is the efficient delivery of f…
View article: Adjudin delays cellular senescence through Sirt3‑mediated attenuation of ROS production
Adjudin delays cellular senescence through Sirt3‑mediated attenuation of ROS production Open
Aging, marked by the physical and functional decline in numerous biological processes, is associated with multiple pathologies including cancer, neurodegenerative diseases and cardiocerebral vascular diseases. The accumulation of reactive …
View article: Sirt3 deficiency impairs neurovascular recovery in ischemic stroke
Sirt3 deficiency impairs neurovascular recovery in ischemic stroke Open
Summary Aims Sirt3 is one member of the NAD + ‐dependent protein deacetylase family and plays crucial roles in diverse aspects of mammalian biological function. Then the role of Sirt3 on ischemia stroke is unknown. Methods To examine the e…
View article: Proceedings of International Conference on Cell Death in Cancer and Toxicology
Proceedings of International Conference on Cell Death in Cancer and Toxicology Open
Apoptotic cells can produce signals to instruct cells in their local environment, including factors that stimulate proliferation.This apoptosis-induced proliferation has been shown to play important roles in tissue regeneration, tumor grow…
View article: Sirt3 Mediates the Inhibitory Effect of Adjudin on Astrocyte Activation and Glial Scar Formation following Ischemic Stroke
Sirt3 Mediates the Inhibitory Effect of Adjudin on Astrocyte Activation and Glial Scar Formation following Ischemic Stroke Open
In response to stroke-induced injury, astrocytes can be activated and form a scar. Inflammation is an essential component for glial scar formation. Previous study has shown that adjudin, a potential Sirt3 activator, could attenuate lipopol…
View article: Adjudin - A Male Contraceptive with Other Biological Activities
Adjudin - A Male Contraceptive with Other Biological Activities Open
Studies on adjudin should be expanded to better understand its biological activities so that it can become a useful drug for treatment of other ailments besides serving as a male contraceptive.