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View article: M. tuberculosis relies on trace oxygen to maintain energy homeostasis and survive in hypoxic environments
M. tuberculosis relies on trace oxygen to maintain energy homeostasis and survive in hypoxic environments Open
View article: Synthesis and Biological Evaluation of Aurachin D Analogues as Inhibitors of <i>Mycobacterium tuberculosis</i> Cytochrome <i>bd</i> Oxidase
Synthesis and Biological Evaluation of Aurachin D Analogues as Inhibitors of <i>Mycobacterium tuberculosis</i> Cytochrome <i>bd</i> Oxidase Open
A revised total synthesis of aurachin D (1a), an isoprenoid quinolone alkaloid that targets Mycobacterium tuberculosis (Mtb) cytochrome bd (cyt-bd) oxidase, was accomplished using an oxazoline ring-openin…
View article: Author response: Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion
Author response: Rate-limiting transport of positively charged arginine residues through the Sec-machinery is integral to the mechanism of protein secretion Open
Article Figures and data Abstract Editor's evaluation Introduction Results Discussion Materials and methods Data availability References Decision letter Author response Article and author information Metrics Abstract Transport of proteins …
View article: Deciphering functional redundancy and energetics of malate oxidation in mycobacteria
Deciphering functional redundancy and energetics of malate oxidation in mycobacteria Open
Oxidation of malate to oxaloacetate, catalyzed by either malate dehydrogenase (Mdh) or malate quinone oxidoreductase (Mqo), is a critical step of the tricarboxylic acid cycle. Both Mqo and Mdh are found in most bacterial genomes, but the l…
View article: An amiloride derivative is active against the F1Fo-ATP synthase and cytochrome bd oxidase of Mycobacterium tuberculosis
An amiloride derivative is active against the F1Fo-ATP synthase and cytochrome bd oxidase of Mycobacterium tuberculosis Open
View article: Alkyltriphenylphosphonium&nbsp;Turns Redox-Cycling Naphthoquinoneimidazoles into Potent Membrane Depolarizers Against <i>Mycobacteria</i>
Alkyltriphenylphosphonium Turns Redox-Cycling Naphthoquinoneimidazoles into Potent Membrane Depolarizers Against <i>Mycobacteria</i> Open
View article: Alkyltriphenylphosphonium turns naphthoquinoneimidazoles into potent membrane depolarizers against <i>mycobacteria</i>
Alkyltriphenylphosphonium turns naphthoquinoneimidazoles into potent membrane depolarizers against <i>mycobacteria</i> Open
Herein, we establish the antimycobacterial activity of trialkylphosphonium-containing naphthoquinoneimidazoles, which exhibited limited redox activity but extensive membrane depolarization that resulted in depletion of ATP.
View article: Emerging connections between gut microbiome bioenergetics and chronic metabolic diseases
Emerging connections between gut microbiome bioenergetics and chronic metabolic diseases Open
The conventional viewpoint of single-celled microbial metabolism fails to adequately depict energy flow at the systems level in host-adapted microbial communities. Emerging paradigms instead support that distinct microbiomes develop interc…
View article: Bacterial respiration keeps amazing us in the 21st century
Bacterial respiration keeps amazing us in the 21st century Open
The last two decades have seen some remarkable discoveries in bacterial bioenergetics. In this article, we highlight how important societal issues such as antimicrobial resistance and sustainable fuel production have led to a renewed focus…
View article: Functionalized Dioxonaphthoimidazoliums: A Redox Cycling Chemotype with Potent Bactericidal Activities against <i>Mycobacterium tuberculosis</i>
Functionalized Dioxonaphthoimidazoliums: A Redox Cycling Chemotype with Potent Bactericidal Activities against <i>Mycobacterium tuberculosis</i> Open
Disruption of redox homeostasis in mycobacteria causes irreversible stress induction and cell death. Here, we report the dioxonaphthoimidazolium scaffold as a novel redox cycling antituberculosis chemotype with potent bactericidal activity…
View article: The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB
The cryo-EM structure of the bd oxidase from M. tuberculosis reveals a unique structural framework and enables rational drug design to combat TB Open
View article: Growth on Formic Acid Is Dependent on Intracellular pH Homeostasis for the Thermoacidophilic Methanotroph Methylacidiphilum sp. RTK17.1
Growth on Formic Acid Is Dependent on Intracellular pH Homeostasis for the Thermoacidophilic Methanotroph Methylacidiphilum sp. RTK17.1 Open
Members of the genus Methylacidiphilum , a clade of metabolically flexible thermoacidophilic methanotrophs from the phylum Verrucomicrobia, can utilize a variety of substrates including methane, methanol, and hydrogen for growth. However, …
View article: Dual inhibition of the terminal oxidases eradicates antibiotic‐tolerant <i>Mycobacterium tuberculosis</i>
Dual inhibition of the terminal oxidases eradicates antibiotic‐tolerant <i>Mycobacterium tuberculosis</i> Open
View article: Antitubercular polyhalogenated phenothiazines and phenoselenazine with reduced binding to CNS receptors
Antitubercular polyhalogenated phenothiazines and phenoselenazine with reduced binding to CNS receptors Open
View article: Multiple Bactericidal Mechanisms of the Zinc Ionophore PBT2
Multiple Bactericidal Mechanisms of the Zinc Ionophore PBT2 Open
More antimicrobials are used in food-producing animals than in humans, and the extensive use of medically important human antimicrobials poses a significant public health threat in the face of rising antimicrobial resistance. Therefore, th…
Predicting nitroimidazole antibiotic resistance mutations in Mycobacterium tuberculosis with protein engineering Open
View article: Extracellular Electron Transfer: Respiratory or Nutrient Homeostasis?
Extracellular Electron Transfer: Respiratory or Nutrient Homeostasis? Open
Exoelectrogens are able to transfer electrons extracellularly, enabling them to respire on insoluble terminal electron acceptors. Extensively studied exoelectrogens, such as Geobacter sulfurreducens and Shewanella oneidensis , are Gram neg…
View article: Disrupting coupling within mycobacterial F-ATP synthases subunit ε causes dysregulated energy production and cell wall biosynthesis
Disrupting coupling within mycobacterial F-ATP synthases subunit ε causes dysregulated energy production and cell wall biosynthesis Open
The dynamic interaction of the N- and C-terminal domains of mycobacterial F-ATP synthase subunit ε is proposed to contribute to efficient coupling of H + -translocation and ATP synthesis. Here, we investigate crosstalk between both subunit…
View article: Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence
Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence Open
To persist when nutrient sources are limited, aerobic soil bacteria metabolize atmospheric hydrogen (H2). This process is the primary sink in the global H2 cycle and supports the productivity of microbes in oligotroph…
View article: Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence
Two uptake hydrogenases differentially interact with the aerobic respiratory chain during mycobacterial growth and persistence Open
Aerobic soil bacteria metabolize atmospheric hydrogen (H 2 ) to persist when nutrient sources are limited. This process is the primary sink in the global H 2 cycle and supports the productivity of microbes in oligotrophic environments. To …
View article: The evolution of nitroimidazole antibiotic resistance in <i>Mycobacterium tuberculosis</i>
The evolution of nitroimidazole antibiotic resistance in <i>Mycobacterium tuberculosis</i> Open
Our inability to predict whether certain mutations will confer antibiotic resistance has made it difficult to rapidly detect the emergence of resistance, identify pre-existing resistant populations and manage our use of antibiotics to effe…
View article: Rate-limiting transport of positive charges through the Sec-machinery is integral to the mechanism of protein transport
Rate-limiting transport of positive charges through the Sec-machinery is integral to the mechanism of protein transport Open
Transport of proteins across and into membranes is a fundamental biological process with the vast majority being conducted by the ubiquitous Sec machinery. In bacteria, this is usually achieved when the SecY-complex engages the cytosolic A…
View article: Alternate quinone coupling in a new class of succinate dehydrogenase may potentiate mycobacterial respiratory control
Alternate quinone coupling in a new class of succinate dehydrogenase may potentiate mycobacterial respiratory control Open
There is a paucity of information on the unique components that pathogens use to form respiratory chains. It is not known why mycobacteria encode multiple succinate dehydrogenases ( SDH s) to perform menaquinone‐linked succinate oxidation,…
View article: FAD-sequestering proteins protect mycobacteria against hypoxic and oxidative stress
FAD-sequestering proteins protect mycobacteria against hypoxic and oxidative stress Open
The ability to persist in the absence of growth triggered by low oxygen levels is a critical process for the survival of mycobacterial species in many environmental niches. MSMEG_5243 (fsq), a gene of unknown function in M…
View article: Ionophoric effects of the antitubercular drug bedaquiline
Ionophoric effects of the antitubercular drug bedaquiline Open
Significance Antibiotics generally target one of five essential cellular functions in bacteria, but many of these targets are now compromised through rapidly spreading antibiotic resistance. Bedaquiline (BDQ), a new FDA-approved antituberc…
View article: Mixotrophy drives niche expansion of verrucomicrobial methanotrophs
Mixotrophy drives niche expansion of verrucomicrobial methanotrophs Open
Aerobic methanotrophic bacteria have evolved a specialist lifestyle dependent on consumption of methane and other short-chain carbon compounds. However, their apparent substrate specialism runs contrary to the high relative abundance of th…
View article: Exploiting the synthetic lethality between terminal respiratory oxidases to kill <i>Mycobacterium tuberculosis</i> and clear host infection
Exploiting the synthetic lethality between terminal respiratory oxidases to kill <i>Mycobacterium tuberculosis</i> and clear host infection Open
Significance New drugs are needed to combat multidrug-resistant tuberculosis. The electron transport chain (ETC) maintains the electrochemical potential across the cytoplasmic membrane and allows production of ATP, the energy currency of a…
View article: Oxidative Phosphorylation as a Target Space for Tuberculosis: Success, Caution, and Future Directions
Oxidative Phosphorylation as a Target Space for Tuberculosis: Success, Caution, and Future Directions Open
The emergence and spread of drug-resistant pathogens, and our inability to develop new antimicrobials to combat resistance, have inspired scientists to seek out new targets for drug development. The Mycobacterium tuberculosis complex is a …
View article: Aerobic H<sub>2</sub>respiration enhances metabolic flexibility of methanotrophic bacteria
Aerobic H<sub>2</sub>respiration enhances metabolic flexibility of methanotrophic bacteria Open
Methanotrophic bacteria are important soil biofilters for the climate-active gas methane. The prevailing opinion is that these bacteria exclusively metabolise single-carbon, and in limited instances, short-chain hydrocarbons for growth. Th…
View article: Activation of type II NADH dehydrogenase by quinolinequinones mediates antitubercular cell death
Activation of type II NADH dehydrogenase by quinolinequinones mediates antitubercular cell death Open
QQ8c targets NDH-2 of the mycobacterial respiratory chain leading to activation of NADH oxidation and generating bactericidal levels of ROS in a manner similar to, but more effectively than, the antimycobacterial drug clofazimine. Our resu…