Kirby R. Campbell
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View article: Optimizing multifunctional fluorescent ligands for intracellular labeling
Optimizing multifunctional fluorescent ligands for intracellular labeling Open
Enzyme-based self-labeling tags enable the covalent attachment of synthetic molecules to proteins inside living cells. A frontier of this field is designing cell-permeable multifunctional ligands that contain fluorophores in combination wi…
View article: Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling
Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling Open
Enzyme-based self-labeling tags enable covalent attachment of synthetic molecules to proteins inside living cells. A frontier of this field is designing multifunctional ligands that contain both fluorophores and affinity tags or pharmacolo…
View article: From Blur to Brilliance: The Ascendance of Advanced Microscopy in Neuronal Cell Biology
From Blur to Brilliance: The Ascendance of Advanced Microscopy in Neuronal Cell Biology Open
The intricate network of the brain's neurons and synapses poses unparalleled challenges for research, distinct from other biological studies. This is particularly true when dissecting how neurons and their functional units work at a cell b…
View article: Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells
Correlative three-dimensional super-resolution and block-face electron microscopy of whole vitreously frozen cells Open
Visualizing whole cells at many scales Cells need to compartmentalize thousands of distinct proteins, but the nanoscale spatial relationship of many proteins to overall intracellular ultrastructure remains poorly understood. Correlated lig…
View article: Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells
Correlative three-dimensional super-resolution and block face electron microscopy of whole vitreously frozen cells Open
Living cells function through the spatial compartmentalization of thousands of distinct proteins serving a multitude of diverse biochemical needs. Correlative super-resolution (SR) fluorescence and electron microscopy (EM) has emerged as a…
View article: Second-harmonic generation microscopy analysis reveals proteoglycan decorin is necessary for proper collagen organization in prostate
Second-harmonic generation microscopy analysis reveals proteoglycan decorin is necessary for proper collagen organization in prostate Open
Collagen remodeling occurs in many prostate pathologies; however, the underlying structural architecture in both normal and diseased prostatic tissues is largely unexplored. Here, we use second-harmonic generation (SHG) microscopy to speci…
View article: Polarization-resolved second harmonic generation imaging of human ovarian cancer
Polarization-resolved second harmonic generation imaging of human ovarian cancer Open
Remodeling of the extracellular matrix in human ovarian cancer can be manifested in increased collagen concentration, changes in alignment within fibrils/fibers and/or up-regulation of different collagen isoforms. We used pixel-based secon…
View article: Assessing local stromal alterations in human ovarian cancer subtypes via second harmonic generation microscopy and analysis
Assessing local stromal alterations in human ovarian cancer subtypes via second harmonic generation microscopy and analysis Open
The collagen architecture in all human ovarian cancers is substantially remodeled, where these alterations are manifested in different fiber widths, fiber patterns, and fibril size and packing. Second harmonic generation (SHG) microscopy h…
View article: 3D second harmonic generation imaging tomography by multi-view excitation
3D second harmonic generation imaging tomography by multi-view excitation Open
Biological tissues have complex 3D collagen fiber architecture that cannot be fully visualized by conventional second harmonic generation (SHG) microscopy due to electric dipole considerations. We have developed a multi-view SHG imaging pl…
View article: Stromal alterations in ovarian cancers via wavelength dependent Second Harmonic Generation microscopy and optical scattering
Stromal alterations in ovarian cancers via wavelength dependent Second Harmonic Generation microscopy and optical scattering Open
SHG and optical scattering measurements provide sub-resolution information and when combined provide superior diagnostic power over clinical imaging modalities. Additionally the measurements are able to delineate the different subtypes of …
View article: 3D texture analysis for classification of second harmonic generation images of human ovarian cancer
3D texture analysis for classification of second harmonic generation images of human ovarian cancer Open
View article: Front Matter: Volume 9329
Front Matter: Volume 9329 Open
View article: Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy
Articular cartilage zonal differentiation via 3D Second-Harmonic Generation imaging microscopy Open
Collectively, these results provide quantitative measurements of structural changes at both the fiber and fibril assembly of the different cartilage zones and reveals structural information not possible by other microscope modalities. This…
View article: Multi-view second-harmonic generation imaging of mouse tail tendon via reflective micro-prisms
Multi-view second-harmonic generation imaging of mouse tail tendon via reflective micro-prisms Open
Here we experimentally show that second-harmonic generation (SHG) imaging is not sensitive to collagen fibers oriented parallel to the direction of laser propagation and, as a consequence, can potentially miss important structural informat…