Kirby T. Leo
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View article: A resource database for protein kinase substrate sequence-preference motifs based on large-scale mass spectrometry data
A resource database for protein kinase substrate sequence-preference motifs based on large-scale mass spectrometry data Open
Background Protein phosphorylation is one of the most prevalent posttranslational modifications involved in molecular control of cellular processes, and is mediated by over 520 protein kinases in humans and other mammals. Identification of…
View article: Engineered peptide-drug conjugate provides sustained protection of retinal ganglion cells with topical administration in rats
Engineered peptide-drug conjugate provides sustained protection of retinal ganglion cells with topical administration in rats Open
View article: Machine learning-driven multifunctional peptide engineering for sustained ocular drug delivery
Machine learning-driven multifunctional peptide engineering for sustained ocular drug delivery Open
Sustained drug delivery strategies have many potential benefits for treating a range of diseases, particularly chronic diseases that require treatment for years. For many chronic ocular diseases, patient adherence to eye drop dosing regime…
View article: Comparing 10x Genomics single-cell 3’ and 5’ assay in short-and long-read sequencing
Comparing 10x Genomics single-cell 3’ and 5’ assay in short-and long-read sequencing Open
Barcoding strategies are fundamental to droplet-based single-cell sequencing, and understanding the biases and caveats between approaches is essential. Here, we comprehensively evaluated both short and long reads of the cDNA obtained throu…
View article: Data resource: vasopressin-regulated protein phosphorylation sites in the collecting duct
Data resource: vasopressin-regulated protein phosphorylation sites in the collecting duct Open
Vasopressin regulates renal water excretion through control of the aquaporin-2 water channel in collecting duct cells. Studies of vasopressin-induced protein phosphorylation have focused mainly on the phosphorylation of aquaporin-2. This s…
View article: Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses
Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses Open
View article: Additional file 5 of Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses
Additional file 5 of Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses Open
Additional file 4: Table S4. Ranking of all kinases in their likelihood of phosphorylating each phosphosite cluster. The final computed output from the Bayesian analysis was represented as probability ratios of Posterior/Initial Prior wher…
View article: Additional file 6 of Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses
Additional file 6 of Bayesian analysis of dynamic phosphoproteomic data identifies protein kinases mediating GPCR responses Open
Additional file 5: Table S5. Nodes and edges for the causal map of vasopressin effects in IMCD. Source nodes were mapped to Target nodes based on literature searches and data from this paper. Information from these sources defined whether …
View article: “ADPKD-omics”: determinants of cyclic AMP levels in renal epithelial cells
“ADPKD-omics”: determinants of cyclic AMP levels in renal epithelial cells Open
View article: Caregiver Preferences for Three-dimensional Printed or Augmented Reality Craniosynostosis Skull Models: A Cross-sectional Survey
Caregiver Preferences for Three-dimensional Printed or Augmented Reality Craniosynostosis Skull Models: A Cross-sectional Survey Open
BACKGROUND: Achieving caregiver understanding of craniosynostosis is a critical component of surgical care. Caregivers must navigate the emotional challenges of a congenital diagnosis, conceptualize skull anatomy, and make decisions on sur…
View article: Systems Biology of the Vasopressin V2 Receptor: New Tools for Discovery of Molecular Actions of a GPCR
Systems Biology of the Vasopressin V2 Receptor: New Tools for Discovery of Molecular Actions of a GPCR Open
Systems biology can be defined as the study of a biological process in which all of the relevant components are investigated together in parallel to discover the mechanism. Although the approach is not new, it has come to the forefront as …
View article: Bayesian identification of candidate transcription factors for the regulation of <i>Aqp2</i> gene expression
Bayesian identification of candidate transcription factors for the regulation of <i>Aqp2</i> gene expression Open
Abetted by the advent of systems biology-based (“-omics”) techniques in the 21st century, there has been a massive expansion of published data relevant to virtually every physiological question. The authors have developed a large-scale dat…
View article: Ion-Complex Microcrystal Formulation Provides Sustained Delivery of a Multimodal Kinase Inhibitor from the Subconjunctival Space for Protection of Retinal Ganglion Cells
Ion-Complex Microcrystal Formulation Provides Sustained Delivery of a Multimodal Kinase Inhibitor from the Subconjunctival Space for Protection of Retinal Ganglion Cells Open
Glaucoma is the leading cause of irreversible blindness worldwide. Elevated intraocular pressure (IOP) is one of the major risk factors for glaucoma onset and progression, and available pharmaceutical interventions are exclusively targeted…
View article: A hypotonic gel-forming eye drop provides enhanced intraocular delivery of a kinase inhibitor with melanin-binding properties for sustained protection of retinal ganglion cells
A hypotonic gel-forming eye drop provides enhanced intraocular delivery of a kinase inhibitor with melanin-binding properties for sustained protection of retinal ganglion cells Open
View article: Strategy to Enhance Dendritic Cell‐Mediated DNA Vaccination in the Lung
Strategy to Enhance Dendritic Cell‐Mediated DNA Vaccination in the Lung Open
We here introduce a new paradigm to promote pulmonary DNA vaccination. Specifically, we demonstrate that nanoparticles designed to rapidly penetrate airway mucus (mucus-penetrating particle or MPP) enhance the delivery of inhaled model DNA…