Kirk L. Hamilton
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View article: Sorting nexins associated with recycling complexes regulate epithelial sodium channel trafficking
Sorting nexins associated with recycling complexes regulate epithelial sodium channel trafficking Open
Cell surface levels of the epithelial sodium channel, ENaC, are controlled by endocytosis and exocytosis pathways. Sorting nexin (SNX) family members facilitate the binding of protein cargo to recycling complexes for sequestration into rec…
View article: The exocyst complex is required for the trafficking and delivery of KCa3.1 to the basolateral membrane of polarized epithelia
The exocyst complex is required for the trafficking and delivery of KCa3.1 to the basolateral membrane of polarized epithelia Open
Our Ussing chamber and immunoblot experiments demonstrate that when subunits of the exocyst complex were transiently knocked down, this significantly reduced the basolateral population and functional expression of KCa3.1. These data sugges…
View article: Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium
Role of SNARE Proteins in the Insertion of KCa3.1 in the Plasma Membrane of a Polarized Epithelium Open
Targeting proteins to a specific membrane is crucial for proper epithelial cell function. KCa3.1, a calcium-activated, intermediate-conductance potassium channel, is targeted to the basolateral membrane (BLM) in epithelial cells. Surprisin…
View article: Differential Binding of AP-1 to the Enhancer of RXFP1 Gene in Normal and Fibrotic Lung Fibroblasts
Differential Binding of AP-1 to the Enhancer of RXFP1 Gene in Normal and Fibrotic Lung Fibroblasts Open
View article: Toll Interacting Protein (TOLLIP) Contributes to Epithelial-Mediated Fibroblast Differentiation by Increasing TGF-β Secretion
Toll Interacting Protein (TOLLIP) Contributes to Epithelial-Mediated Fibroblast Differentiation by Increasing TGF-β Secretion Open
View article: Soluble RAGE Is a Biomarker for Idiopathic Pulmonary Fibrosis
Soluble RAGE Is a Biomarker for Idiopathic Pulmonary Fibrosis Open
INTRODUCTION:The receptor for advanced glycosylated end products (RAGE) mediates inflammation and injury response through ligand binding and signal transduction.A soluble form (sRAGE) of RAGE, a decoy receptor, is readily detectable in per…
View article: Retromer is involved in epithelial Na<sup>+</sup> channel trafficking
Retromer is involved in epithelial Na<sup>+</sup> channel trafficking Open
The epithelial Na + channel (ENaC) located at the apical membrane in many epithelia is the rate-limiting step for Na + reabsorption. Tight regulation of the plasma membrane population of ENaC is required, as hypertension or hypotension may…
View article: <i>AJP-Cell Physiology</i> begins a Theme of Reviews on “New Frontiers in Protein Trafficking”
<i>AJP-Cell Physiology</i> begins a Theme of Reviews on “New Frontiers in Protein Trafficking” Open
View article: Conditional KCa3.1-transgene induction in murine skin produces pruritic eczematous dermatitis with severe epidermal hyperplasia and hyperkeratosis
Conditional KCa3.1-transgene induction in murine skin produces pruritic eczematous dermatitis with severe epidermal hyperplasia and hyperkeratosis Open
Ion channels have recently attracted attention as potential mediators of skin disease. Here, we explored the consequences of genetically encoded induction of the cell volume-regulating Ca2+-activated KCa3.1 channel (Kcnn4) for murine epide…
View article: Conditional KCa3.1-Transgene Induction in Murine Skin Produces Pruritic Eczematous Dermatitis With Severe Epidermal Hyperplasia and Hyperkeratosis
Conditional KCa3.1-Transgene Induction in Murine Skin Produces Pruritic Eczematous Dermatitis With Severe Epidermal Hyperplasia and Hyperkeratosis Open
Ion channels have recently attracted attention as potential mediators of skin disease. Here, we explored the consequences of genetically encoded induction of the cell volume-regulating Ca 2+ -activated KCa3.1 channel ( Kcnn4 ) for murine e…
View article: KCa3.1 Transgene Induction in Murine Intestinal Epithelium Causes Duodenal Chyme Accumulation and Impairs Duodenal Contractility
KCa3.1 Transgene Induction in Murine Intestinal Epithelium Causes Duodenal Chyme Accumulation and Impairs Duodenal Contractility Open
The epithelial intermediate-conductance calcium/calmodulin-regulated KCa3.1 channel is considered to be a regulator of intestine function by controlling chloride secretion and water/salt balance. Yet, little is known about the functional i…
View article: A tool for multiscale modelling of the nephron
A tool for multiscale modelling of the nephron Open
Slides from my presentation at the VPH Conference 2010, 30 Sep - 1 Oct, 2010, Brussels, Belgium.
View article: Interacting with a multiscale renal nephron model
Interacting with a multiscale renal nephron model Open
Slides from my presentation at the 6th World Congress of Biomechanics, 1-6 August 2010, Singapore.
View article: Modelling epithelial transport
Modelling epithelial transport Open
Slides from my presentation at ICBME 2013, 4-7 December, Town Plaza, U-Town, National University of Singapore, Singapore.
View article: Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23
Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23 Open
We previously demonstrated endocytosis of KCa2.3 is caveolin-1-, dynamin II- and Rab5-dependent. KCa2.3 then enters Rab35/EPI64C- and RME-1-containing recycling endosomes and is returned to the plasma membrane (PM). Herein, we report on th…
View article: Pharmacological activation of TRPV4 produces immediate cell damage and induction of apoptosis in human melanoma cells and HaCaT keratinocytes
Pharmacological activation of TRPV4 produces immediate cell damage and induction of apoptosis in human melanoma cells and HaCaT keratinocytes Open
Our study showed that TRPV4 channels were functionally expressed in human melanoma cell lines and in human keratinocytes. Pharmacological TRPV4 activation in human melanoma cells and keratinocytes caused severe cellular disarrangement, nec…
View article: <i>AJP-Cell Physiology</i> begins landmark reviews in cell physiology: an editorial from the senior editors of <i>AJP-Cell Physiology</i>
<i>AJP-Cell Physiology</i> begins landmark reviews in cell physiology: an editorial from the senior editors of <i>AJP-Cell Physiology</i> Open
upon the conversion of the American Journal of Physiology into a suite of Journals that embraced different areas of Physiology, either tissue-specific, or, as for AJP-Cell Physiology, covering the realm of cells and molecules
View article: Modulation of Retrograde Trafficking of KCa3.1 in a Polarized Epithelium
Modulation of Retrograde Trafficking of KCa3.1 in a Polarized Epithelium Open
In epithelia, the intermediate conductance, Ca2+-activated K+ channel (KCa3.1) is targeted to the basolateral membrane (BLM) where this channel plays numerous roles in absorption and secretion. A growing body of resea…
View article: The Role of the Cytoskeleton and Myosin-Vc in the Targeting of KCa3.1 to the Basolateral Membrane of Polarized Epithelial Cells
The Role of the Cytoskeleton and Myosin-Vc in the Targeting of KCa3.1 to the Basolateral Membrane of Polarized Epithelial Cells Open
Understanding the targeting of KCa3.1 to the basolateral membrane (BLM) of polarized epithelial cells is still emerging. Here, we examined the role of the cytoskeleton (microtubules and microfilaments) and Myosin-Vc (Myo-Vc) in the targeti…
View article: 50 Years of renal physiology from one man and the perfused tubule: Maurice B. Burg
50 Years of renal physiology from one man and the perfused tubule: Maurice B. Burg Open
Technical advancements in research techniques in science are made in slow increments. Even so, large advances from insight and hard work of an individual with a single technique can have astonishing ramifications. Here, we examine the impa…
View article: <i>New life in overactive bladder</i>. Focus on “Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function”
<i>New life in overactive bladder</i>. Focus on “Novel regulatory mechanism in human urinary bladder: central role of transient receptor potential melastatin 4 channels in detrusor smooth muscle function” Open
INDIVIDUALS ARE FACED WITH varying aspects of health problems that have an impact on their quality of life.Suffering a nonfatal heart attack certainly changes one's appreciation of life, which may result in a vigilant awareness of blood pr…
View article: The Role of MicroRNAs in the Regulation of K+ Channels in Epithelial Tissue
The Role of MicroRNAs in the Regulation of K+ Channels in Epithelial Tissue Open
Our understanding of the modulation of proteins has shifted in direction with the discovery of microRNAs (miRs) over twenty years ago. MiRs are now in the "limelight" as these non-coding pieces of RNA (generally ~22 nucleotides long) resul…
View article: Computational Tools for the Creation, Simulation and Dissemination of Epithelial Cell Models
Computational Tools for the Creation, Simulation and Dissemination of Epithelial Cell Models Open
My poster presented at Experimental Biology 2015.\nAbstract: \nWe have developed a software suite for the creation, simulation, and dissemination of epithelial cell models. The suite, known as GET (Generalized Epithelial Transport), is fre…
View article: Computational Tools for the Creation, Simulation and Dissemination of Epithelial Cell Models
Computational Tools for the Creation, Simulation and Dissemination of Epithelial Cell Models Open