Kirsten Deprey
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View article: 5-Dihydroxyboryluridine enhances cytosolic penetration of antisense oligonucleotides
5-Dihydroxyboryluridine enhances cytosolic penetration of antisense oligonucleotides Open
Boronic acid functionalized synthetic antisense oligonucleotides demonstrate enhanced cellular uptake and biological activity, constituting a novel approach for delivery of therapeutic oligonucleotides.
View article: Quantitative Measurement of Cytosolic and Nuclear Penetration of Oligonucleotide Therapeutics
Quantitative Measurement of Cytosolic and Nuclear Penetration of Oligonucleotide Therapeutics Open
A major obstacle in the development of effective oligonucleotide therapeutics is a lack of understanding about their cytosolic and nuclear penetration. To address this problem, we have applied the chloroalkane penetration assay (CAPA) to o…
View article: Stapled β-Hairpins Featuring 4-Mercaptoproline
Stapled β-Hairpins Featuring 4-Mercaptoproline Open
Peptides constrained by intramolecular cross-links, especially stapled α-helices, have emerged as versatile scaffolds for drug development. However, there are fewer examples of similarly constrained scaffolds for other secondary structures…
View article: A critical analysis of methods used to investigate the cellular uptake and subcellular localization of RNA therapeutics
A critical analysis of methods used to investigate the cellular uptake and subcellular localization of RNA therapeutics Open
RNA therapeutics are a promising strategy to treat genetic diseases caused by the overexpression or aberrant splicing of a specific protein. The field has seen major strides in the clinical efficacy of this class of molecules, largely due …
View article: Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold
Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold Open
Cyclotides are macrocyclic peptides with exceptionally stable structures and have been reported to penetrate cells, making them promising scaffolds for the delivery of inhibitory peptides to target intracellular proteins. However, their ce…
View article: Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold
Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold Open
Cyclotides are macrocyclic peptides that have exceptionally stable structures and been reported to penetrate cells, making them promising scaffolds for the delivery of peptide inhibitory sequences to target intracellular proteins. However,…
View article: Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold
Cellular Uptake and Cytosolic Delivery of a Cyclic Cystine Knot Scaffold Open
Cyclotides are macrocyclic peptides that have exceptionally stable structures and been reported to penetrate cells, making them promising scaffolds for the delivery of peptide inhibitory sequences to target intracellular proteins. However,…
View article: Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization
Trapped! A Critical Evaluation of Methods for Measuring Total Cellular Uptake versus Cytosolic Localization Open
Biomolecules have many properties that make them promising for intracellular therapeutic applications, but delivery remains a key challenge because large biomolecules cannot easily enter the cytosol. Furthermore, quantification of total in…
View article: Cell Penetration Profiling Using the Chloroalkane Penetration Assay
Cell Penetration Profiling Using the Chloroalkane Penetration Assay Open
Biotherapeutics are a promising class of molecules in drug discovery, but they are often limited to extracellular targets due to their poor cell penetration. High-throughput cell penetration assays are required for the optimization of biot…