Kirti Prakash
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View article: Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications
Resolution in super-resolution microscopy – facts, artifacts, technological advancements and biological applications Open
Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well …
View article: CLEAR-IT: Contrastive Learning to Capture the Immune Composition of Tumor Microenvironments
CLEAR-IT: Contrastive Learning to Capture the Immune Composition of Tumor Microenvironments Open
Accurate phenotyping of cells in the tumor microenvironment is crucial for understanding cancer biology and developing effective therapies. However, current methods require precise cell segmentations and struggle to generalize across diffe…
View article: Unveiling the limits of precision in iterative MINFLUX
Unveiling the limits of precision in iterative MINFLUX Open
In single‐molecule microscopy, a big question is how precisely we can estimate the location of a single molecule. Our research shows that by using iterative localisation microscopy and factoring in the prior information, we can boost preci…
View article: Correlative super‐resolution microscopy with deep UV reactivation
Correlative super‐resolution microscopy with deep UV reactivation Open
Correlative super‐resolution microscopy has the potential to accurately visualize and validate new biological structures past the diffraction limit. However, combining different super‐resolution modalities, such as deterministic stimulated…
View article: Opinions on imaging and cutting-edge developments in light microscopy for biomedical applications
Opinions on imaging and cutting-edge developments in light microscopy for biomedical applications Open
Optical microscopy has revolutionized the field of biology, enabling researchers to explore the intricate details of biological structures and processes with unprecedented clarity.Over the past few decades, significant strides have been ma…
View article: Correlative superresolution microscopy with deep UV reactivation
Correlative superresolution microscopy with deep UV reactivation Open
Correlative superresolution microscopy has the potential to accurately visualize and validate new biological structures past the diffraction limit. However, combining different superresolution modalities, such as deterministic stimulated e…
View article: Open microscopy in the life sciences: quo vadis?
Open microscopy in the life sciences: quo vadis? Open
Light microscopy enables researchers to observe cellular mechanisms with high spatial and temporal resolution. However, the increasing complexity of current imaging technologies, coupled with financial constraints of potential users, hampe…
View article: Super-resolution microscopy: a brief history and new avenues
Super-resolution microscopy: a brief history and new avenues Open
Super-resolution microscopy (SRM) is a fast-developing field that encompasses fluorescence imaging techniques with the capability to resolve objects below the classical diffraction limit of optical resolution. Acknowledged with the Nobel p…
View article: At the molecular resolution with MINFLUX?
At the molecular resolution with MINFLUX? Open
MINFLUX is purported as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1–3 nm in fixed and living cells. Though the claim of molecular resolution is attractive, I am concerned whether…
View article: Open microscopy in the life sciences: Quo Vadis?
Open microscopy in the life sciences: Quo Vadis? Open
Light microscopy allows observing cellular features and objects with sub-micrometer resolution. As such, light microscopy has been playing a fundamental role in the life sciences for more than a hundred years. Fueled by the availability of…
View article: Assessment of 3D MINFLUX data for quantitative structural biology in cells
Assessment of 3D MINFLUX data for quantitative structural biology in cells Open
Reanalysed data for "Assessment of 3D MINFLUX data for quantitative structural biology in cells" https://www.biorxiv.org/content/10.1101/2021.08.10.455294v1 Contact Hell lab for the raw data https://www.mpibpc.mpg.de/hell
View article: Assessment of 3D MINFLUX data for quantitative structural biology in cells
Assessment of 3D MINFLUX data for quantitative structural biology in cells Open
Reanalysed data for "Assessment of 3D MINFLUX data for quantitative structural biology in cells" https://www.biorxiv.org/content/10.1101/2021.08.10.455294v1 Contact Hell lab for the raw data https://www.mpibpc.mpg.de/hell
View article: Assessment of 3D MINFLUX data for quantitative structural biology in cells
Assessment of 3D MINFLUX data for quantitative structural biology in cells Open
Reanalysed data for "Assessment of 3D MINFLUX data for quantitative structural biology in cells" https://www.biorxiv.org/content/10.1101/2021.08.10.455294v1 Contact Hell lab for the raw data https://www.mpibpc.mpg.de/hell
View article: Assessment of 3D MINFLUX data for quantitative structural biology in cells
Assessment of 3D MINFLUX data for quantitative structural biology in cells Open
MINFLUX is a promising new development in single-molecule localization microscopy, claiming a resolution of 1-3 nm in living and fixed biological specimens. While MINFLUX can achieve very high localisation precision, quantitative analysis …
View article: Super-resolution structured illumination microscopy: past, present and future.
Super-resolution structured illumination microscopy: past, present and future. Open
Structured illumination microscopy (SIM) has emerged as an essential technique for three-dimensional (3D) and live-cell super-resolution imaging. However, to date, there has not been a dedicated workshop or journal issue covering the vario…
View article: At the molecular resolution with MINFLUX?
At the molecular resolution with MINFLUX? Open
Gwosch et al. (2020) and Balzarotti et al. (2017) purport MINFLUX as the next revolutionary fluorescence microscopy technique claiming a spatial resolution in the range of 1-3 nm in fixed and living cells. Though the claim of molecular res…
View article: Supplementary material from "Laser-free super-resolution microscopy"
Supplementary material from "Laser-free super-resolution microscopy" Open
We report that high-density single-molecule super-resolution microscopy can be achieved with a conventional epifluorescence microscope set-up and a Mercury arc lamp. The configuration termed as laser-free super-resolution microscopy (LFSM)…
View article: Laser-free super-resolution microscopy
Laser-free super-resolution microscopy Open
Raw data for "Laser-free super-resolution microscopy" https://www.biorxiv.org/content/10.1101/121061v3.full.pdf
View article: Laser-free super-resolution microscopy
Laser-free super-resolution microscopy Open
Raw data for "Laser-free super-resolution microscopy" https://www.biorxiv.org/content/10.1101/121061v3.full.pdf
View article: Deciphering the histone code to build the genome structure
Deciphering the histone code to build the genome structure Open
Histones are punctuated with small chemical modifications that alter their interaction with DNA. One attractive hypothesis stipulates that certain combinations of these histone modifications may function, alone or together, as a part of a …
View article: Laser-free super-resolution microscopy
Laser-free super-resolution microscopy Open
We report that high-density single-molecule super-resolution microscopy can be achieved with a conventional epifluorescence microscope setup and a Mercury arc lamp. The configuration termed as laser-free super-resolution microscopy (LFSM),…
View article: High-density super-resolution microscopy with an incoherent light source and a conventional epifluorescence microscope setup
High-density super-resolution microscopy with an incoherent light source and a conventional epifluorescence microscope setup Open
We report that single-molecule superresolution microscopy can be achieved with a conventional epifluorescence microscope setup and a Mercury arc lamp. The configuration termed as Omnipresent Localisation Microscope (OLM), is an extension o…
View article: Histone Code and Higher-Order Chromatin Folding: A Hypothesis
Histone Code and Higher-Order Chromatin Folding: A Hypothesis Open
Histone modifications alone or in combination are thought to modulate chromatin structure and function; a concept termed histone code. By combining evidence from several studies, we investigated if the histone code can play a role in highe…
View article: Histone code and higher-order chromatin folding: A hypothesis
Histone code and higher-order chromatin folding: A hypothesis Open
Histone modifications alone or in combination are thought to modulate chromatin structure and function; a concept termed histone code. By combining evidence from several studies, we investigated if the histone code can play a role in highe…