Kishore Garapati
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View article: Further delineation of defects in MRPS2 causing human OXPHOS deficiency and early developmental abnormalities in zebrafish
Further delineation of defects in MRPS2 causing human OXPHOS deficiency and early developmental abnormalities in zebrafish Open
Mitochondrial ribosomal protein-small 2 ( MRPS2 ) encodes a vital structural protein essential for assembling mitoribosomal small subunit and thus mitochondrial translation. Any defect in mitochondrial translation impacts OXPHOS activity a…
View article: Defining albumin as a glycoprotein with multiple N-linked glycosylation sites
Defining albumin as a glycoprotein with multiple N-linked glycosylation sites Open
Background Glycosylation is an enzyme-catalyzed post-translational modification that is distinct from glycation and is present on a majority of plasma proteins. N -glycosylation occurs on asparagine residues predominantly within canonical …
View article: A complement C4–derived glycopeptide is a biomarker for PMM2-CDG
A complement C4–derived glycopeptide is a biomarker for PMM2-CDG Open
BACKGROUNDDiagnosis of PMM2-CDG, the most common congenital disorder of glycosylation (CDG), relies on measuring carbohydrate-deficient transferrin (CDT) and genetic testing. CDT tests have false negatives and may normalize with age. Site-…
View article: Global O-glycoproteome enrichment and analysis enabled by a combinatorial enzymatic workflow
Global O-glycoproteome enrichment and analysis enabled by a combinatorial enzymatic workflow Open
View article: Recent progress in mass spectrometry-based urinary proteomics
Recent progress in mass spectrometry-based urinary proteomics Open
Serum or plasma is frequently utilized in biomedical research; however, its application is impeded by the requirement for invasive sample collection. The non-invasive nature of urine collection makes it an attractive alternative for diseas…
View article: sBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides
sBioSITe enables sensitive identification of the cell surface proteome through direct enrichment of biotinylated peptides Open
Background Cell surface proteins perform critical functions related to immune response, signal transduction, cell–cell interactions, and cell migration. Expression of specific cell surface proteins can determine cell-type identity, and can…
View article: Identification of SARS-CoV-2 from Human Lung Formalin-Fixed Paraffin-Embedded Tissue Sections Using Mass Spectrometry
Identification of SARS-CoV-2 from Human Lung Formalin-Fixed Paraffin-Embedded Tissue Sections Using Mass Spectrometry Open
View article: Whole Exome Sequencing Reveals Novel Variants in Unexplained Erythrocytosis
Whole Exome Sequencing Reveals Novel Variants in Unexplained Erythrocytosis Open
Erythrocytosis is characterized by an increase in red cells in peripheral blood. Polycythemia vera, the commonest primary erythrocytosis, results from pathogenic variants in JAK2 in ∼98% of cases. Although some variants have been re…
View article: Multiomics single timepoint measurements to predict severe COVID-19 – Authors' reply
Multiomics single timepoint measurements to predict severe COVID-19 – Authors' reply Open
View article: Correction: Digging deeper into the immunopeptidome: characterization of post‑translationally modified peptides presented by MHC I
Correction: Digging deeper into the immunopeptidome: characterization of post‑translationally modified peptides presented by MHC I Open
View article: Mass spectrometric analysis of chondroitin sulfate-linked peptides
Mass spectrometric analysis of chondroitin sulfate-linked peptides Open
Chondroitin sulfate proteoglycans (CSPGs) are extracellular matrix components composed of linear glycosaminoglycan (GAG) side chains attached to a core protein. CSPGs play a vital role in neurodevelopment, signal transduction, cellular pro…
View article: <scp>TRIT1</scp> defect leads to a recognizable phenotype of myoclonic epilepsy, speech delay, strabismus, progressive spasticity, and normal lactate levels
<span>TRIT1</span> defect leads to a recognizable phenotype of myoclonic epilepsy, speech delay, strabismus, progressive spasticity, and normal lactate levels Open
TRIT1 defect is a rare, autosomal‐recessive disorder of transcription, initially described as a condition with developmental delay, myoclonic seizures, and abnormal mitochondrial function. Currently, only 13 patients have been reported. We…
View article: Development of a multiomics model for identification of predictive biomarkers for COVID-19 severity: a retrospective cohort study
Development of a multiomics model for identification of predictive biomarkers for COVID-19 severity: a retrospective cohort study Open
Eric and Wendy Schmidt.
View article: Proteomic alterations in extracellular vesicles induced by oncogenic <i>PIK3CA</i> mutations
Proteomic alterations in extracellular vesicles induced by oncogenic <i>PIK3CA</i> mutations Open
PIK3CA is one of the most frequently mutated genes in human cancers, with the two most prevalent activating mutations being E545K and H1047R. Although the altered intracellular signaling pathways in these cells have been described, the eff…
View article: TMT-Based Multiplexed Quantitation of <i>N</i>-Glycopeptides Reveals Glycoproteome Remodeling Induced by Oncogenic Mutations
TMT-Based Multiplexed Quantitation of <i>N</i>-Glycopeptides Reveals Glycoproteome Remodeling Induced by Oncogenic Mutations Open
Glycoproteomics, or the simultaneous characterization of glycans and their attached peptides, is increasingly being employed to generate catalogs of glycopeptides on a large scale. Nevertheless, quantitative glycoproteomics remains challen…
View article: Sorbitol Is a Severity Biomarker for <scp>PMM2‐CDG</scp> with Therapeutic Implications
Sorbitol Is a Severity Biomarker for <span>PMM2‐CDG</span> with Therapeutic Implications Open
Objective Epalrestat, an aldose reductase inhibitor increases phosphomannomutase (PMM) enzyme activity in a PMM2‐congenital disorders of glycosylation (CDG) worm model. Epalrestat also decreases sorbitol level in diabetic neuropathy. We ev…
View article: Digging deeper into the immunopeptidome: characterization of post-translationally modified peptides presented by MHC I
Digging deeper into the immunopeptidome: characterization of post-translationally modified peptides presented by MHC I Open
View article: Extensive heterogeneity of glycopeptides in plasma revealed by deep glycoproteomic analysis using size-exclusion chromatography
Extensive heterogeneity of glycopeptides in plasma revealed by deep glycoproteomic analysis using size-exclusion chromatography Open
Size-exclusion chromatography facilitates simultaneous enrichment and fractionation of N -glycopeptides. Combining it with improved LC-MS/MS analysis achieves deep coverage of plasma N -glycoproteome.
View article: Proteomic Signature of Host Response to SARS-CoV-2 Infection in the Nasopharynx
Proteomic Signature of Host Response to SARS-CoV-2 Infection in the Nasopharynx Open