G. G. Siu
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View article: Ultrasound-Assisted Extraction: Unlocking the Antibacterial Potential of Coptis chinensis Franch. Against ESBL-Producing Enterobacterales
Ultrasound-Assisted Extraction: Unlocking the Antibacterial Potential of Coptis chinensis Franch. Against ESBL-Producing Enterobacterales Open
The global rise of multidrug-resistant Extended Spectrum β-Lactamase Producing-Enterobacterales (ESBL-PE) poses a critical threat to public health, driving the urgent need for alternative therapeutic approaches. This study evaluates the an…
View article: Rapidly Progressing Melioidosis Outbreak in City Center Zoo, Hong Kong, 2024
Rapidly Progressing Melioidosis Outbreak in City Center Zoo, Hong Kong, 2024 Open
In October 2024, twelve primates from 4 species died of sepsis at the Hong Kong Zoological and Botanical Gardens. Postmortem examinations and microbiological analyses confirmed Burkholderia pseudomallei infection. Phylogenetic analysis rev…
View article: Zika virus and the fetal-maternal interface: deciphering the mechanisms of placental infection and implications for pregnancy outcomes
Zika virus and the fetal-maternal interface: deciphering the mechanisms of placental infection and implications for pregnancy outcomes Open
Zika virus (ZIKV) is an emerging flavivirus primarily transmitted by Aedes mosquitoes. It has gained significant attention due to its potential teratogenic effects during pregnancy, particularly the increased risk of severe congenital outc…
View article: Genetic characterization of influenza A viruses circulating in Hong Kong, China, 2023: the first influenza epidemic after the lifting of 2.5 years of COVID-19 non-pharmaceutical interventions
Genetic characterization of influenza A viruses circulating in Hong Kong, China, 2023: the first influenza epidemic after the lifting of 2.5 years of COVID-19 non-pharmaceutical interventions Open
During the COVID-19 pandemic, stringent public health measures led to historically low influenza activity in Hong Kong. However, after these interventions were relaxed in 2023, Influenza A viruses (IAV), including A(H1N1)pdm09 and A(H3N2),…
View article: Optimizing and Evaluating Nanopore-Based Targeted and Metagenomic Sequencing Workflows for Rapid Diagnosis of Acute Invasive Infections from Normally Sterile Body Fluids
Optimizing and Evaluating Nanopore-Based Targeted and Metagenomic Sequencing Workflows for Rapid Diagnosis of Acute Invasive Infections from Normally Sterile Body Fluids Open
Rapid and accurate pathogen identification is critical for managing acute invasive infections. Conventional culture methods are time-consuming, delaying effective treatment. Nanopore sequencing offers real-time, long-read capabilities suit…
View article: High-quality genome assembly and comparative analysis reveal extensive genomic variation in Talaromyces marneffei
High-quality genome assembly and comparative analysis reveal extensive genomic variation in Talaromyces marneffei Open
Talaromyces marneffei is a dimorphic fungus that transitions from a filamentous form at 25 °C to a pathogenic yeast form at 37 °C, demonstrating pathogenicity mostly in immunocompromised individuals, such as those with human immunodeficien…
View article: A Novel Digital PCR Assay for Accurate Detection and Differentiation of Focal and Non-Focal Subtypes of Mesenchymal–Epithelial Transition (MET) Gene Amplification in Lung Cancer
A Novel Digital PCR Assay for Accurate Detection and Differentiation of Focal and Non-Focal Subtypes of Mesenchymal–Epithelial Transition (MET) Gene Amplification in Lung Cancer Open
Background/Objectives: Mesenchymal–epithelial transition (MET) gene amplification is a critical biomarker in non-small cell lung cancer (NSCLC), significantly influencing treatment decisions and prognostic evaluations. However, current det…
View article: A Novel Digital PCR Assay for Accurate Detection and Differentiation of Focal and Non-Focal Subtypes of <i>MET</i> Amplification in Lung Cancer
A Novel Digital PCR Assay for Accurate Detection and Differentiation of Focal and Non-Focal Subtypes of <i>MET</i> Amplification in Lung Cancer Open
Mesenchymal–epithelial transition (MET) gene amplification is a critical biomarker in non-small cell lung cancer (NSCLC), significantly influencing treatment decisions and prognostic evaluations. However, current detection methods such as …
View article: Scrofimicrobium appendicitidis sp. nov., isolated from a patient with ruptured appendicitis
Scrofimicrobium appendicitidis sp. nov., isolated from a patient with ruptured appendicitis Open
A clinical isolate, R131, was isolated from the peritoneal swab of a patient who suffered from ruptured appendicitis with abscess and gangrene in Hong Kong in 2018. Cells are facultatively anaerobic, non-motile, Gram-positive coccobacilli.…
View article: Use of the PRECEDE-PROCEED Model in Piloting Vaccine Promotion and Infection Self-Protection: Intervention Development and Effectiveness Examination
Use of the PRECEDE-PROCEED Model in Piloting Vaccine Promotion and Infection Self-Protection: Intervention Development and Effectiveness Examination Open
Objectives: This study aimed to tailor and pilot a health education program using the PRECEDE-PROCEED model to promote vaccination and enhance self-protective behaviors against COVID-19 in Hong Kong populations. Study design: Quasi-experim…
View article: Characteristics and pathogenicity of Vibrio alginolyticus SWS causing high mortality in mud crab (Scylla serrata) aquaculture in Hong Kong
Characteristics and pathogenicity of Vibrio alginolyticus SWS causing high mortality in mud crab (Scylla serrata) aquaculture in Hong Kong Open
Introduction Vibrio alginolyticus is a Gram-negative, rod-shaped bacterium belonging to the family of Vibrionaceae , a common pathogen in aquaculture animals, However, studies on its impact on Scylla serrata (mud crabs) are limited. In thi…
View article: Transmission Patterns of Co-Circulation of Omicron Sub-Lineages in Hong Kong SAR, China, a City with Rigorous Social Distancing Measures, in 2022
Transmission Patterns of Co-Circulation of Omicron Sub-Lineages in Hong Kong SAR, China, a City with Rigorous Social Distancing Measures, in 2022 Open
Objective: This study aimed to characterize the changing landscape of circulating SARS-CoV-2 lineages in the local community of Hong Kong throughout 2022. We examined how adjustments to quarantine arrangements influenced the transmission p…
View article: Transmission Patterns of Co-Circulation of Omicron Sub-Lineages in Hong Kong SAR, China, a City with Rigorous Social Distancing Measures, in 2022
Transmission Patterns of Co-Circulation of Omicron Sub-Lineages in Hong Kong SAR, China, a City with Rigorous Social Distancing Measures, in 2022 Open
Objective: The study aimed to characterize the changing landscape of circulating SARS-CoV-2 lineages in the local community of Hong Kong throughout 2022. We examined how adjustments to quarantine arrangements influenced the transmission pa…
View article: Genetic mechanisms of co-emergence of INH-resistant <i>Mycobacterium tuberculosis</i> strains during the standard course of antituberculosis therapy
Genetic mechanisms of co-emergence of INH-resistant <i>Mycobacterium tuberculosis</i> strains during the standard course of antituberculosis therapy Open
The incidence of isoniazid (INH) resistant Mycobacterium tuberculosis is increasing globally. This study aimed to identify the molecular mechanisms behind the development of INH resistance in M. tuberculosis strains collected from the same…
View article: Community Engagement in Vaccination Promotion: Systematic Review and Meta-Analysis
Community Engagement in Vaccination Promotion: Systematic Review and Meta-Analysis Open
Background Community engagement plays a vital role in global immunization strategies, offering the potential to overcome vaccination hesitancy and enhance vaccination confidence. Although there is significant backing for community engageme…
View article: The clinical utility of Nanopore 16S rRNA gene sequencing for direct bacterial identification in normally sterile body fluids
The clinical utility of Nanopore 16S rRNA gene sequencing for direct bacterial identification in normally sterile body fluids Open
The prolonged incubation period of traditional culture methods leads to a delay in diagnosing invasive infections. Nanopore 16S rRNA gene sequencing (Nanopore 16S) offers a potential rapid diagnostic approach for directly identifying bacte…
View article: Comprehensive Identification of Pathogenic Microbes and Antimicrobial Resistance Genes in Food Products Using Nanopore Sequencing-Based Metagenomics
Comprehensive Identification of Pathogenic Microbes and Antimicrobial Resistance Genes in Food Products Using Nanopore Sequencing-Based Metagenomics Open
Foodborne pathogens, particularly antimicrobial-resistant (AMR) bacteria, remain a significant threat to global health. Conventional culture-based approaches for detecting infectious agents are limited in scope and time-consuming. Metageno…
View article: Spatiotemporal Distribution of Tuberculosis in the Oromia Region of Ethiopia: A Hotspot Analysis
Spatiotemporal Distribution of Tuberculosis in the Oromia Region of Ethiopia: A Hotspot Analysis Open
Tuberculosis (TB) is a major public health concern in low- and middle-income countries including Ethiopia. This study aimed to assess the spatiotemporal distribution of TB and identify TB risk factors in Ethiopia’s Oromia region. Descripti…
View article: Long-Read Sequencing with Hierarchical Clustering for Antiretroviral Resistance Profiling of Mixed Human Immunodeficiency Virus Quasispecies
Long-Read Sequencing with Hierarchical Clustering for Antiretroviral Resistance Profiling of Mixed Human Immunodeficiency Virus Quasispecies Open
Background HIV infections often develop drug resistance mutations (DRMs), which can increase the risk of virological failure. However, it has been difficult to determine if minor mutations occur in the same genome or in different virions u…
View article: Resurgence of Omicron BA.2 in SARS-CoV-2 infection-naive Hong Kong
Resurgence of Omicron BA.2 in SARS-CoV-2 infection-naive Hong Kong Open
Hong Kong experienced a surge of Omicron BA.2 infections in early 2022, resulting in one of the highest per-capita death rates of COVID-19. The outbreak occurred in a dense population with low immunity towards natural SARS-CoV-2 infection,…
View article: Probable airborne transmission of <i>Burkholderia pseudomallei</i> causing an urban outbreak of melioidosis during typhoon season in Hong Kong, China
Probable airborne transmission of <i>Burkholderia pseudomallei</i> causing an urban outbreak of melioidosis during typhoon season in Hong Kong, China Open
Between January 2015 and October 2022, 38 patients with culture-confirmed melioidosis were identified in the Kowloon West (KW) Region, Hong Kong. Notably, 30 of them were clustered in the Sham Shui Po (SSP) district, which covers an estima…
View article: Identification of microbial community in the urban environment: The concordance between conventional culture and nanopore 16S rRNA sequencing
Identification of microbial community in the urban environment: The concordance between conventional culture and nanopore 16S rRNA sequencing Open
Introduction Microbes in the built environment have been implicated as a source of infectious diseases. Bacterial culture is the standard method for assessing the risk of exposure to pathogens in urban environments, but this method only ac…
View article: Probable airborne transmission of <i>Burkholderia pseudomallei</i> causing an urban outbreak of melioidosis during typhoon season in Hong Kong, China
Probable airborne transmission of <i>Burkholderia pseudomallei</i> causing an urban outbreak of melioidosis during typhoon season in Hong Kong, China Open
Between January 2015 and October 2022, 38 patients with culture-confirmed melioidosis were identified in the Kowloon West (KW) Region, Hong Kong. Notably, 30 of them were clustered in the Sham Shui Po (SSP) district, which covers an estima…
View article: Evaluation of<i>Mycobacterium Tuberculosis</i>enrichment in metagenomic samples using ONT adaptive sequencing and amplicon sequencing for identification and variant calling
Evaluation of<i>Mycobacterium Tuberculosis</i>enrichment in metagenomic samples using ONT adaptive sequencing and amplicon sequencing for identification and variant calling Open
Sensitive detection of Mycobacterium Tuberculosis (TB) in small percentages in metagenomic samples is essential for microbial classification and drug resistance prediction, and assisting in diagnosis and treatment planning. However, tradit…
View article: Resurgence of Omicron BA.2 in SARS-CoV-2 infection-naive Hong Kong
Resurgence of Omicron BA.2 in SARS-CoV-2 infection-naive Hong Kong Open
After keeping infections at bay for two years, Hong Kong experienced a surge of Omicron BA.2 infections in early 2022 that overwhelmed the health care system, isolation facilities, and contact tracing capacity, leading to one of the highes…
View article: Understanding spatiotemporal symptom onset risk of Omicron BA.1, BA.2 and hamster-related Delta AY.127
Understanding spatiotemporal symptom onset risk of Omicron BA.1, BA.2 and hamster-related Delta AY.127 Open
Purpose Investigation of the community-level symptomatic onset risk regarding severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern, is crucial to the pandemic control in the new normal. Methods Investigated in t…