Koichiro Gen
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View article: Optimization of a protocol for fertilized egg production in Japanese eel using recombinant gonadotropins, LHRHa, pimozide, and 17α-hydroxyprogesterone
Optimization of a protocol for fertilized egg production in Japanese eel using recombinant gonadotropins, LHRHa, pimozide, and 17α-hydroxyprogesterone Open
Recently, two types of Japanese eel recombinant gonadotropins (rGths), recombinant follicle-stimulating hormone (rFsh) and luteinizing hormone (rLh), have been successfully produced. However, the protocol for obtaining fertilized eggs usin…
View article: Effect of a long photoperiod on the timing of spawning in the Pacific bluefin tuna, Thunnus orientalis
Effect of a long photoperiod on the timing of spawning in the Pacific bluefin tuna, Thunnus orientalis Open
In this study, we examined the effects of a long photoperiod on the reproduction of Pacific bluefin tuna (Thunnus orientalis) in a land-based tank. Three-year-old fish were shifted from a short (10.5 L:13.5D) to long (15 L:9D) photoperiod …
View article: Effect of water temperature on the survival and growth of hatchery-reared juvenile Pacific bluefin tuna <i>Thunnus orientalis</i>
Effect of water temperature on the survival and growth of hatchery-reared juvenile Pacific bluefin tuna <i>Thunnus orientalis</i> Open
飼育水温の違いがクロマグロ人工種苗の生残と成長に及ぼす影響を調べた。異なる水温(17, 20, 23, および26℃)での飼育試験の結果,生残率は低水温区で高くなる傾向が認められた。体サイズは低水温区で小型化する傾向にあるものの,23℃区の成長は26℃区と同等であり,飼料効率は23℃区で最も高い値が得られた。本研究の結果から,クロマグロ人工種苗は17℃以上の海域で育成可能であり,23℃付近が成長により適した水温であると示唆された。
View article: Establishment of cell-lines stably expressing recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone using CHO-DG44 cells: fully induced ovarian development at different modes
Establishment of cell-lines stably expressing recombinant Japanese eel follicle-stimulating hormone and luteinizing hormone using CHO-DG44 cells: fully induced ovarian development at different modes Open
The gonadotropins (Gth), follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh), play central roles in gametogenesis in vertebrates. However, available information on their differential actions in teleost, especially in vivo , is …
View article: Transcriptome characterization of gonadal sex differentiation in Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel)
Transcriptome characterization of gonadal sex differentiation in Pacific bluefin tuna, Thunnus orientalis (Temminck et Schlegel) Open
Tunas (genus Thunnus ) are one of the most ecologically and commercially important fish worldwide. To establish a biological basis for reproduction in this globally essential species, we have recently studied crucial reproductive aspects o…
View article: Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2
Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2 Open
Genome editing is a technology that can remarkably accelerate crop and animal breeding via artificial induction of desired traits with high accuracy. This study aimed to develop a chub mackerel variety with reduced aggression using an expe…
View article: First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor
First Data on Sexual Dimorphic Growth of Cultured Pacific Bluefin Tuna, Thunnus orientalis (Temminck et Schlegel), and Its Sex Manipulation by Treatment with an Aromatase Inhibitor Open
Tuna (genus Thunnus), particularly Pacific bluefin tuna (T. orientalis; PBT), are commercially important fish in the aquaculture industry worldwide. The objective of this study was to investigate sexual dimorphism in the growth performance…
View article: Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2
Development of a chub mackerel with less-aggressive fry stage by genome editing of arginine vasotocin receptor V1a2 Open
Genome editing is a technology that can remarkably accelerate crop and animal breeding via artificial induction of desired traits with high accuracy. This study aimed to develop a chub mackerel variety with reduced aggression using an expe…
View article: Normal hatching rate estimation for bulk samples of Pacific bluefin tuna (Thunnus orientalis) eggs using deep learning
Normal hatching rate estimation for bulk samples of Pacific bluefin tuna (Thunnus orientalis) eggs using deep learning Open
View article: Prediction of the Sex-Associated Genomic Region in Tunas (Thunnus Fishes)
Prediction of the Sex-Associated Genomic Region in Tunas (Thunnus Fishes) Open
Fish species have a variety of sex determination systems. Tunas (genus Thunnus) have an XY genetic sex determination system. However, the Y chromosome or responsible locus has not yet been identified in males. In a previous study, a female…
View article: TALEN-Mediated Gene Editing of slc24a5 (Solute Carrier Family 24, Member 5) in Kawakawa, Euthynnus affinis
TALEN-Mediated Gene Editing of slc24a5 (Solute Carrier Family 24, Member 5) in Kawakawa, Euthynnus affinis Open
Transcription activator-like effector (TALE) nucleases (TALENs) mediated gene editing methods are becoming popular and have revealed the staggering complexity of genome control during development. Here, we present a simple and efficient ge…
View article: Consumer Evaluation of Genome-edited Food Products: A Case of Fully Farmed Tuna
Consumer Evaluation of Genome-edited Food Products: A Case of Fully Farmed Tuna Open
クロマグロの天然資源管理が進む中，天然資源に依存しない完全養殖やゲノム編集技術の開発による家魚化が進む．本稿では，ゲノム編集技術を使って作出された完全養殖に適したマグロを事例に消費者受容を分析した．選択実験の結果，ゲノム編集技術の利用に否定的な消費者が多く，技術の応用により完全養殖マグロの価格をどの程度下げられるかが普及のカギとなる．ただし技術になじみのない回答者も多く，技術への理解を深めてもらうことや技術が資源管理に有用であることを周知すること，倫理的消費に関心をもっても…
View article: Vision-based egg quality prediction in Pacific bluefin tuna (Thunnus orientalis) by deep neural network
Vision-based egg quality prediction in Pacific bluefin tuna (Thunnus orientalis) by deep neural network Open
View article: Histological Evidence of Multiple Spawning in Wild Female Japanese Eel <i>Anguilla japonica</i>.
Histological Evidence of Multiple Spawning in Wild Female Japanese Eel <i>Anguilla japonica</i>. Open
The post ovulatory follicle (POF) is an important and reliable tissue structure used to investigate the spawning history in teleost fish. Fresh POFs shortly after spawning are comprised of cellular (follicular cells) and acellular (basemen…
View article: Author Correction: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
Author Correction: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis) Open
View article: Insulin-like growth factors 1 and 2 regulate gene expression and enzymatic activity of cyp17a1 in ovarian follicles of the yellowtail, Seriola quinqueradiata
Insulin-like growth factors 1 and 2 regulate gene expression and enzymatic activity of cyp17a1 in ovarian follicles of the yellowtail, Seriola quinqueradiata Open
There is accumulating evidence that insulin-like growth factors (IGFs), primary mediators of somatic growth, play an important role in fish reproduction. Previously, we showed that IGF-1 and IGF-2 are expressed in the ovarian follicle cell…
View article: Effect of tank shape on survival and growth of Pacific bluefin tuna Thunnus orientalis larvae
Effect of tank shape on survival and growth of Pacific bluefin tuna Thunnus orientalis larvae Open
View article: インシュリン様成長因子1と2はブリ,Seriola quinqueradiataの卵胞におけるcyp17a1の遺伝子発現と酵素活性を調節する【JST・京大機械翻訳】
インシュリン様成長因子1と2はブリ,Seriola quinqueradiataの卵胞におけるcyp17a1の遺伝子発現と酵素活性を調節する【JST・京大機械翻訳】 Open
View article: Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis)
Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis) Open
View article: Current status and future of genome editing technologies for breeding of agricultural products
Current status and future of genome editing technologies for breeding of agricultural products Open
View article: Quantitative comparisons of maternal transcripts related to cell division between good and poor quality eggs from artificially matured Japanese eel Anguilla japonica
Quantitative comparisons of maternal transcripts related to cell division between good and poor quality eggs from artificially matured Japanese eel Anguilla japonica Open
The current method of artificial egg production in Japanese eel does not always yield good quality eggs, but the molecular basis for the decrease in egg quality remains unclear. In this study, we compared the levels of 74 maternal transcri…