Koichiro Otake
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View article: <i>Rhizobium rhizogenes</i>-mediated hairy-root transformation of daughter plants from the model strawberry <i>Fragaria vesca</i>’s stolons
<i>Rhizobium rhizogenes</i>-mediated hairy-root transformation of daughter plants from the model strawberry <i>Fragaria vesca</i>’s stolons Open
Strawberry, a member of the Fragaria genus within the Rosaceae family, is one of the most cherished fruits worldwide. This perennial herbaceous plant also serves as a model for studying the Rosaceae family. Despite the complex polyp…
View article: <i>De novo</i> induction of a <scp>DNA</scp>–histone <scp>H3K9</scp> methylation loop on synthetic human repetitive <scp>DNA</scp> in cultured tobacco cells
<i>De novo</i> induction of a <span>DNA</span>–histone <span>H3K9</span> methylation loop on synthetic human repetitive <span>DNA</span> in cultured tobacco cells Open
SUMMARY Genetic modifications in plants are crucial tools for fundamental and applied research. Transgene expression usually varies among independent lines or their progeny and is associated with the chromatin structure of the insertion si…
View article: Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation
Combination of CENP-B Box Positive and Negative Synthetic Alpha Satellite Repeats Improves De Novo Human Artificial Chromosome Formation Open
Human artificial chromosomes (HACs) can be formed de novo by introducing large (>30 kb) centromeric sequences consisting of highly repeated 171-bp alpha satellite (alphoid) DNA into HT1080 cells. However, only a subset of transformed cells…
View article: Introduction of a long synthetic repetitive DNA sequence into cultured tobacco cells
Introduction of a long synthetic repetitive DNA sequence into cultured tobacco cells Open
Genome information has been accumulated for many species, and these genes and regulatory sequences are expected to be applied in plants by enhancing or creating new metabolic pathways. We hypothesized that manipulating a long array of repe…
View article: CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly
CENP-B creates alternative epigenetic chromatin states permissive for CENP-A or heterochromatin assembly Open
CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induc…
View article: Human artificial chromosome: Chromatin assembly mechanisms and CENP-B
Human artificial chromosome: Chromatin assembly mechanisms and CENP-B Open
The centromere is a specialized chromosomal locus required for accurate chromosome segregation. Heterochromatin also assembles around centromere chromatin and forms a base that supports sister chromatid cohesion until anaphase begins. Both…
View article: KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation
KAT7/HBO1/MYST2 Regulates CENP-A Chromatin Assembly by Antagonizing Suv39h1-Mediated Centromere Inactivation Open
View article: CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly
CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly Open
Although it is generally accepted that chromatin containing the histone H3 variant CENP-A is an epigenetic mark maintaining centromere identity, the pathways leading to the formation and maintenance of centromere chromatin remain unclear. …
View article: Stable complex formation of CENP-B with the CENP-A nucleosome
Stable complex formation of CENP-B with the CENP-A nucleosome Open
CENP-A and CENP-B are major components of centromeric chromatin. CENP-A is the histone H3 variant, which forms the centromere-specific nucleosome. CENP-B specifically binds to the CENP-B box DNA sequence on the centromere-specific repetiti…