Masato Koike
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View article: Human midbrain organoids reveal the characteristics of axonal mitochondria specific to dopaminergic neurons
Human midbrain organoids reveal the characteristics of axonal mitochondria specific to dopaminergic neurons Open
Mitochondrial dysfunction and abnormalities in mitochondrial quality control contribute to the development of neurodegenerative diseases. Parkinson’s disease is a neurodegenerative disease that causes motor problems mainly due to the loss …
View article: Longitudinal Excitation and Lamellar Feedforward Inhibition of the Hippocampal <scp>CA3</scp> to <scp>CA1</scp> Projections
Longitudinal Excitation and Lamellar Feedforward Inhibition of the Hippocampal <span>CA3</span> to <span>CA1</span> Projections Open
The hippocampus is a longitudinally extended brain structure characterized by its classic trisynaptic circuit, comprising the dentate gyrus (DG), CA3, and CA1 subregions, evident in transverse slices and consistent throughout the hippocamp…
View article: The difference in immunohistochemical reactivity of monoclonal antibodies against amino-terminal residues of amyloid-β peptide
The difference in immunohistochemical reactivity of monoclonal antibodies against amino-terminal residues of amyloid-β peptide Open
Immunohistochemistry for amyloid-β (Aβ) peptide is an indispensable method for Alzheimer’s disease (AD) research. Despite a wide variety of available antibodies against the peptides, the difference of immunohistochemical reactivity is not …
View article: Effect of Contrast Media Type on Renal Function in Percutaneous Coronary Intervention (PCI): A Propensity Score-Matched Analysis Considering Contrast Media Volume and Preoperative Renal Function
Effect of Contrast Media Type on Renal Function in Percutaneous Coronary Intervention (PCI): A Propensity Score-Matched Analysis Considering Contrast Media Volume and Preoperative Renal Function Open
Objective To compare the effects of two low-osmolar contrast media (LOCM), iohexol and iomeprol, on renal function in patients undergoing percutaneous coronary intervention (PCI), as measured by estimated glomerular filtration rate (eGFR).…
View article: A phosphorylation-driven functional switch in p62 bodies coordinates autophagic clearance of ubiquitinated proteins
A phosphorylation-driven functional switch in p62 bodies coordinates autophagic clearance of ubiquitinated proteins Open
p62/SQSTM1 self-assembles with polyubiquitin into liquid-like condensates (“p62 bodies”) that function as stress-signaling hubs and selective autophagy cargo. We show that TBK1-dependent phosphorylation at Ser403 acts as a tunable rheostat…
View article: A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification
A three dimensional immunolabeling method with peroxidase-fused nanobodies and fluorochromized tyramide-glucose oxidase signal amplification Open
Three dimensional immunohistochemistry (3D-IHC), immunolabeling of 3D tissues, reveals the spatial organization of molecular and cellular assemblies in the context of the tissue architecture. Deep and rapid penetration of antibodies into 3…
View article: A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification
A Three Dimensional Immunolabeling Method with Peroxidase-fused Nanobodies and Fluorochromized Tyramide-Glucose Oxidase Signal Amplification Open
Three dimensional immunohistochemistry (3D-IHC), immunolabeling of 3D tissues, reveals the spatial organization of molecular and cellular assemblies in the context of the tissue architecture. Deep and rapid penetration of antibodies into 3…
View article: Golgi pH homeostasis stabilizes the lysosomal membrane through<i>N</i>-glycosylation of membrane proteins
Golgi pH homeostasis stabilizes the lysosomal membrane through<i>N</i>-glycosylation of membrane proteins Open
Protein glycosylation plays a vital role in various cellular functions, many of which occur within the Golgi apparatus. The Golgi pH regulator (GPHR) is essential for the proper functioning of the Golgi apparatus. The lysosomal membrane co…
View article: Reviewer #2 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Reviewer #2 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: Reviewer #1 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Reviewer #1 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: Reviewer #3 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Reviewer #3 (Public Review): Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia
Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia Open
Protein spinster homolog 1 (SPNS1) is a lysosomal transporter of lysophospholipids and sphingosine, which has recently been identified to be mutated in patients with neurodegeneration. However, its physiological role, especially in the ner…
View article: SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential
SOD2 orchestrates redox homeostasis in intervertebral discs: A novel insight into oxidative stress-mediated degeneration and therapeutic potential Open
Low back pain (LBP) is a pervasive global health concern, primarily associated with intervertebral disc (IVD) degeneration. Although oxidative stress has been shown to contribute to IVD degeneration, the underlying mechanisms remain undete…
View article: Reduced ER-mitochondrial contact sites and mitochondrial Ca2+ flux in PRKN-mutant patient tyrosine hydroxylase reporter iPSC lines
Reduced ER-mitochondrial contact sites and mitochondrial Ca2+ flux in PRKN-mutant patient tyrosine hydroxylase reporter iPSC lines Open
Endoplasmic reticulum-mitochondrial contact sites (ERMCS) play an important role in mitochondrial dynamics, calcium signaling, and autophagy. Disruption of the ERMCS has been linked to several neurodegenerative diseases, including Alzheime…
View article: Genome-wide screening for regulators of degradation of insulin secretory granules with a fluorescent reporter
Genome-wide screening for regulators of degradation of insulin secretory granules with a fluorescent reporter Open
Insulin is essential in controlling blood glucose levels, and its synthesis and secretion have been well investigated. In contrast, how insulin secretory granules (ISGs) are degraded in pancreatic beta cells remains largely unknown. To cla…
View article: Importin α Characterizes a Micronuclear Environment Associated with Genomic Instability in Human Cancer Cells
Importin α Characterizes a Micronuclear Environment Associated with Genomic Instability in Human Cancer Cells Open
Micronuclei (MN) are membrane-enclosed chromatin bodies and hallmarks of genome instability. Here, we report that importin α, a key nuclear transport factor, is highly concentrated in a distinct subset of MN in cultured human cancer cells.…
View article: Holographic optical grating and method for optimizing monochromator configuration
Holographic optical grating and method for optimizing monochromator configuration Open
This invention comprises a novel apparatus for recording a holographic groove pattern on a diffraction grating blank. The recording apparatus is configured using newly developed groups of analytical equations. The invention further compris…
View article: Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle
Specific AAV2/PHP.eB-mediated gene transduction of CA2 pyramidal cells via injection into the lateral ventricle Open
Given its limited accessibility, the CA2 area has been less investigated compared to other subregions of the hippocampus. While the development of transgenic mice expressing Cre recombinase in the CA2 has revealed unique features of this a…
View article: Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis
Fluorochromized tyramide-glucose oxidase as a multiplex fluorescent tyramide signal amplification system for histochemical analysis Open
Tyramide signal amplification (TSA) is a highly sensitive method for histochemical analysis. Previously, we reported a TSA system, biotinyl tyramine-glucose oxidase (BT-GO), for bright-filed imaging. Here, we develop fluorochromized tyrami…
View article: Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue
Protocol for multi-scale light microscopy/electron microscopy neuronal imaging in mouse brain tissue Open
An imaging technique across multiple spatial scales is required for extracting structural information on neurons with processes of meter scale length and specialized nanoscale structures. Here, we present a protocol combining multi-scale l…
View article: FT-GO: a multiplex fluorescent tyramide signal amplification system for histochemical analysis
FT-GO: a multiplex fluorescent tyramide signal amplification system for histochemical analysis Open
Tyramide signal amplification (TSA) is a highly sensitive method for histochemical analysis. Previously, we reported a TSA system, BT-GO, for bright-filed imaging. Here, we develop FT-GO (Fluorochromized Tyramide-Glucose Oxidase) as a mult…
View article: Clinical Efficacy of Melon GliSODin® for the Treatment of Aging-Related Dysfunction in Motor Organs—A Double Blind, Randomized Placebo-Controlled Study
Clinical Efficacy of Melon GliSODin® for the Treatment of Aging-Related Dysfunction in Motor Organs—A Double Blind, Randomized Placebo-Controlled Study Open
Background: Locomotive syndrome is a concept proposed in Japan involving decreased mobility due to osteoarthritis, osteoporosis, and sarcopenia. This double-blind, randomized study aimed to investigate the effects of superoxide dismutase (…
View article: An optimized protocol for immuno-electron microscopy of endogenous LC3
An optimized protocol for immuno-electron microscopy of endogenous LC3 Open
MAP1LC3/LC3 (microtubule associated protein 1 light chain 3) is widely used as marker of autophagic compartments at different stages of maturation. Electron microscopy (EM) combined with immunolabeling is the only technique that can reveal…