Koji Nakade
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View article: Author response: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Author response: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
View article: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of …
View article: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of …
View article: Author response: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Author response: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
View article: Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization
Generation of MBP-tdTomato reporter human induced pluripotent stem cell line for live myelin visualization Open
Myelin basic protein (MBP) is a major component of the myelin sheaths of oligodendrocytes in the central nervous system and Schwann cells of the peripheral nervous system. Here we generated heterozygous fluorescent reporter of MBP gene in …
View article: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of …
View article: Reviewer #3 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Reviewer #3 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various type of tissues in regenerative medicine; however, a scalable culture system that can precisely control the cell status for hiPSCs is not developed…
View article: Reviewer #1 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Reviewer #1 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various type of tissues in regenerative medicine; however, a scalable culture system that can precisely control the cell status for hiPSCs is not developed…
View article: Reviewer #2 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Reviewer #2 (Public Review): Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various type of tissues in regenerative medicine; however, a scalable culture system that can precisely control the cell status for hiPSCs is not developed…
View article: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various type of tissues in regenerative medicine; however, a scalable culture system that can precisely control the cell status for hiPSCs is not developed…
View article: Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system
Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system Open
Although recent advances in genome editing technology with homology-directed repair have enabled the insertion of various reporter genes into the genome of mammalian cells, the efficiency is still low due to the random insertion of donor v…
View article: Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation
Complete suspension culture of human induced pluripotent stem cells supplemented with suppressors of spontaneous differentiation Open
Human induced pluripotent stem cells (hiPSCs) are promising resources for producing various types of tissues in regenerative medicine; however, the improvement in a scalable culture system that can precisely control the cellular status of …
View article: Efficient Selection of Knocked-In Pluripotent Stem Cells Using Dual Cassettes of Cellular Suicide System
Efficient Selection of Knocked-In Pluripotent Stem Cells Using Dual Cassettes of Cellular Suicide System Open
View article: Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing
Generation of two ISL1-tdTomato reporter human induced pluripotent stem cell lines using CRISPR-Cas9 genome editing Open
View article: Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation”
Response to correspondence on “Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation” Open
View article: Dimethyl sulfoxide stimulates the AhR-Jdp2 axis to control ROS accumulation in mouse embryonic fibroblasts
Dimethyl sulfoxide stimulates the AhR-Jdp2 axis to control ROS accumulation in mouse embryonic fibroblasts Open
View article: Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
View article: Additional file 5: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 5: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S5. The details and the results of the 330 unique loci attempted for ssODNÂ knock-in projects (i.e. point mutation knock-in or short-tag insertion projects). (XLSX 14 kb)
View article: Additional file 3: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 3: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S3. The results of the two-donor floxing approach via electroporation for 7 unique loci. (XLSX 11 kb)
View article: Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 1: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S1. The guide RNA and singlestranded Oligonucleotide DNA sequences, their concentrations and the length of genomic regions floxed (in bp) reported in this study. (XLSX 29 kb)
View article: Additional file 7: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 7: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S7. The Evaluation of the modified method of two-donor floxing method (sequential delivery approach) reported in the Horii et al. 2017 report. (XLSX 11 kb)
View article: Additional file 2: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 2: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S2. The results of the two-donor floxing approach via microinjection for 49 unique loci. (XLSX 21 kb)
View article: Additional file 4: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 4: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S4. Overall efficiency of the two-donor (2sgRNA-2ssODN) floxing method of generating the cKO alleles (XLSX 9 kb)
View article: Additional file 6: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation
Additional file 6: of Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation Open
Table S6. The details and the results of alternate methods of floxing, tested on 18 loci. (XLSX 23 kb)
View article: Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology
Re-Evaluating One-step Generation of Mice Carrying Conditional Alleles by CRISPR-Cas9-Mediated Genome Editing Technology Open
CRISPR-Cas9 gene editing technology has considerably facilitated the generation of mouse knockout alleles, relieving many of the cumbersome and time-consuming steps of traditional mouse embryonic stem cell technology. However, the generati…
View article: Jun dimerization protein 2 controls hypoxia‐induced replicative senescence via both the p16<sup>Ink4a</sup>‐pRb and Arf‐p53 pathways
Jun dimerization protein 2 controls hypoxia‐induced replicative senescence via both the p16<sup>Ink4a</sup>‐pRb and Arf‐p53 pathways Open
The main regulators of replicative senescence in mice are p16 Ink4a and Arf, inhibitors of cell cycle progression. Jun dimerization protein 2 (JDP2)‐deficient mouse embryonic fibroblasts are resistant to replicative senescence through recr…
View article: An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes
An easy method for preparation of Cre-loxP regulated fluorescent adenoviral expression vectors and its application for direct reprogramming into hepatocytes Open
The recombinant adenoviral gene expression system is a powerful tool for gene delivery. However, it is difficult to obtain high titers of infectious virus, principally due to the toxicity of the expressed gene which affects on virus replic…