Koki Kamiya
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View article: Riboswitch-Controlled Lipid Remodeling Enables Functional Membrane Asymmetry in Artificial Cells
Riboswitch-Controlled Lipid Remodeling Enables Functional Membrane Asymmetry in Artificial Cells Open
Dynamic regulation of lipid membrane composition is fundamental to living cells; however, synthetic analogs capable of such regulation remain scarce. Here, we present an artificial cell platform in which riboswitch-mediated expression of p…
View article: Patterning of layered semiconductor GeS <sub>2</sub> by laser photooxidation and water immersion
Patterning of layered semiconductor GeS <sub>2</sub> by laser photooxidation and water immersion Open
A lithography-free photopatterning method for layered GeS 2 is demonstrated, eliminating resist residue problems, enabling low-power laser patterning with minimal heat damage, and employing water itself as an environmentally friendly etcha…
View article: Cell-Penetrating Peptide-Mediated Biomolecule Transportation in Artificial Lipid Vesicles and Living Cells
Cell-Penetrating Peptide-Mediated Biomolecule Transportation in Artificial Lipid Vesicles and Living Cells Open
Signal transduction and homeostasis are regulated by complex protein interactions in the intracellular environment. Therefore, the transportation of impermeable macromolecules (nucleic acids, proteins, and drugs) that control protein inter…
View article: Development, Reliability and Validity of Scales for Assessment of Walking Level in Convalescent Rehabilitation Ward
Development, Reliability and Validity of Scales for Assessment of Walking Level in Convalescent Rehabilitation Ward Open
はじめに:回復期リハビリテーション病棟において歩行能力の評価は重要な課題である.しかし,従来の歩行能力評価尺度には,歩行困難である重症例の座位・立位能力や,歩行修正自立例での歩行補助具の違いを評価することには限界がある.そこでわれわれは,これらの限界を補う目的で,新たに歩行レベル評価尺度Walking LEVEL Scale(WaLS)を開発し,その信頼性と妥当性について検証することを本研究の目的とした.
View article: Controlled Self‐Assembly of Vesicles by Electrospray Deposition
Controlled Self‐Assembly of Vesicles by Electrospray Deposition Open
The self‐assembly of amphiphilic molecules produces structures of diverse dimensions, encompassing micelles, tubules, lamellae, and vesicles. This study focuses on elucidating the controllability of amphiphilic lipid molecule self‐assembly…
View article: Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film
Formation of Cell-Sized Liposomes Incorporating a β-Barrel-Structured Porin through Rehydration of a Phospholipid-Membrane Protein Dried Film Open
Giant unilamellar vesicles (GUVs) integrated with membrane proteins (proteo-GUVs) are attractive tools for visualizing membrane protein functions such as enzyme reactions and molecular transportation. In the dehydration-rehydration method,…
View article: Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changed lipids
Efficiency of transcription and translation of cell-free protein synthesis systems in cell-sized lipid vesicles with changed lipids Open
To develop artificial cell models that mimic living cells, cell-sized lipid vesicles encapsulating cell-free protein synthesis (CFPS) systems are useful for translation and regulation or artificial gene circuits for vesicle–vesicle communi…
View article: Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes
Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes Open
Membrane proteins play an important role in key cellular functions, such as signal transduction, apoptosis, and metabolism. Therefore, structural and functional studies of these proteins are essential in fields such as fundamental biology,…
View article: Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation
Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation Open
Lipid vesicles, which mimic cell membranes in structure and components, have been used to study the origin of life and artificial cell construction. A different approach to developing cell-mimicking systems focuses on the formation of prot…
View article: Cell-sized asymmetric phospholipid–amphiphilic protein vesicles with growth, fission, and molecule transportation
Cell-sized asymmetric phospholipid–amphiphilic protein vesicles with growth, fission, and molecule transportation Open
Lipid vesicles, which mimic cell membranes in structure and components, have been widely used to study the origin of life and artificial cell construction. A different approach to developing cell-mimicking systems focuses on the formation …
View article: Formation and function of bacterial outer membrane proteins- incorporated liposomes using an in vitro translation system
Formation and function of bacterial outer membrane proteins- incorporated liposomes using an in vitro translation system Open
Outer membrane proteins (OMPs), located on the outer membrane of gram-negative bacteria, have a β-strand structure and form nanopores, which allow passage of ions, sugars, and small molecules. Recently, OMPs have been used as sensing eleme…
View article: Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules
Investigation of Fusion between Nanosized Lipid Vesicles and a Lipid Monolayer Toward Formation of Giant Lipid Vesicles with Various Kinds of Biomolecules Open
We determined the properties of fusion between large unilamellar vesicles (LUVs) and the lipid monolayer by measuring the fluorescence intensity of rhodamine-conjugated phospholipids in cell-sized lipid vesicles. The charge of LUVs (contai…
View article: Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers
Highly sensitive VOC detectors using insect olfactory receptors reconstituted into lipid bilayers Open
A highly sensitive odorant sensor was developed by using insect olfactory receptors reconstituted into a lipid bilayer membrane.
View article: Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution
Efficient Lipid Bilayer Formation by Dipping Lipid-Loaded Microperforated Sheet in Aqueous Solution Open
This paper describes a method for a bilayer lipid membrane (BLM) formation using a perforated sheet along with an open chamber. Microscopic observation of the formed membrane showed a typical droplet interface bilayer. We proved that the f…
View article: Construction of Hazard Map for Traffic Accidents in Hilly and Mountainous Area Using 3D Measurement for Road Alignment
Construction of Hazard Map for Traffic Accidents in Hilly and Mountainous Area Using 3D Measurement for Road Alignment Open
In previous paper, evaluation model for four-wheeled vehicle accident risk in hilly and mountainous area was considered using sideslip limitation speed by 3D measurement for road alignment. This paper aimed to improve the risk evaluation m…
View article: A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange
A Lipid-Bilayer-On-A-Cup Device for Pumpless Sample Exchange Open
Lipid-bilayer devices have been studied for on-site sensors in the fields of diagnosis, food and environmental monitoring, and safety/security inspection. In this paper, we propose a lipid-bilayer-on-a-cup device for serial sample measurem…
View article: Lipid Bilayer Array: Rapid and Resilient Detection of Toxin Pore Formation Using a Lipid Bilayer Array (Small 49/2020)
Lipid Bilayer Array: Rapid and Resilient Detection of Toxin Pore Formation Using a Lipid Bilayer Array (Small 49/2020) Open
In article number 2005550, Shoji Takeuchi and co-workers present a parallel lipid bilayer array with integrating series resistors for sensitive and resilient detection of toxin pore formation, illustrated by electrical signatures. This arr…
View article: Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology
Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology Open
Giant lipid vesicles or liposomes are primarily composed of phospholipids and form a lipid bilayer structurally similar to that of the cell membrane. These vesicles, like living cells, are 5–100 μm in diameter and can be easily observed us…
View article: Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila
Molecular and Functional Analysis of Pore-Forming Toxin Monalysin From Entomopathogenic Bacterium Pseudomonas entomophila Open
Pseudomonas entomophila is a highly pathogenic bacterium that infects insects. It is also used as a suitable model pathogen to analyze Drosophila's innate immunity. P. entomophila's virulence is largely derived from Monalysin, a β-barrel p…
View article: A pumpless solution exchange system for nanopore sensors
A pumpless solution exchange system for nanopore sensors Open
This paper proposes a nanopore-based sensor exploiting the solution exchange of a droplet-based lipid bilayer driven by a superabsorbent polymer. Biological nanopores are candidates for use in portable sensors because of their potential to…
View article: Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method
Formation of vesicles-in-a-vesicle with asymmetric lipid components using a pulsed-jet flow method Open
We develop a cell-sized asymmetric lipid vesicle system containing small-sized asymmetric lipid vesicles using microfluidic technology.