Kosuke Takeya
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View article: PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis
PHI-1, an Endogenous Inhibitor Protein for Protein Phosphatase-1 and a Pan-Cancer Marker, Regulates Raf-1 Proteostasis Open
Raf-1, a multifunctional kinase, regulates various cellular processes, including cell proliferation, apoptosis, and migration, by phosphorylating MAPK/ERK kinase and interacting with specific kinases. Cellular Raf-1 activity is intricately…
View article: Dysregulation of the progranulin-driven autophagy-lysosomal pathway mediates secretion of the nuclear protein TDP-43
Dysregulation of the progranulin-driven autophagy-lysosomal pathway mediates secretion of the nuclear protein TDP-43 Open
The cytoplasmic accumulation of the nuclear protein transactive response DNA-binding protein 43 kDa (TDP-43) has been linked to the progression of amyotrophic lateral sclerosis and frontotemporal lobar degeneration. TDP-43 secreted into th…
View article: Possible roles of N- and C-terminal unstructured tails of CPI-17 in regulating Ca<sup>2+</sup> sensitization force of smooth muscle
Possible roles of N- and C-terminal unstructured tails of CPI-17 in regulating Ca<sup>2+</sup> sensitization force of smooth muscle Open
CPI-17 regulates the myosin phosphatase and mediates the agonist-induced contraction of smooth muscle. PKC and ROCK phosphorylate CPI-17 at Thr38 leading to a conformational change of the central inhibitory domain (PHIN domain). The N- and…
View article: Streptolysin O: a novel mediator of endothelial dysfunction
Streptolysin O: a novel mediator of endothelial dysfunction Open
Microbial imbalance (dysbiosis) is closely linked to several diseases including cardiovascular diseases. Gram-positive Streptococcus genus is reported to be increased in feces of spontaneous hypertensive rat (SHR) with increased intestinal…
View article: Kinase Activity-Tagged Western Blotting Assay
Kinase Activity-Tagged Western Blotting Assay Open
Determining cellular activities of protein kinases is a fundamental step for characterizing pathophysiological cell signaling pathways. Here, we optimized a nonradioactive method that detects protein kinases in tissues or cells after separ…
View article: Bacterial toxin, streptolysin O caused vascular endothelial dysfunction: Relationship between dysbiosis and hypertension
Bacterial toxin, streptolysin O caused vascular endothelial dysfunction: Relationship between dysbiosis and hypertension Open
Emerging evidences provide a microbial imbalance (dysbiosis) is linked to several diseases including cardiovascular diseases. It has been reported that Gram-positive Streptococcus genus is increased in feces of spontaneous hypertensive rat…
View article: Expression of troponin subunits in the rat renal afferent arteriole
Expression of troponin subunits in the rat renal afferent arteriole Open
Vascular smooth muscle cells of the renal afferent arteriole are unusual in that they must be able to contract very rapidly in response to a sudden increase in systemic blood pressure in order to protect the downstream glomerular capillari…
View article: Inhibitory effects of rubratoxin A, a potent inhibitor of protein phosphatase 2, on the Ca<sup>2+</sup>-dependent contraction of skinned carotid artery from guinea pig
Inhibitory effects of rubratoxin A, a potent inhibitor of protein phosphatase 2, on the Ca<sup>2+</sup>-dependent contraction of skinned carotid artery from guinea pig Open
Rubratoxin A, a potent inhibitor of PP2A, is known to suppress smooth muscle contraction. The inhibitory role of PP2A in smooth muscle contraction is still unclear. In order to clarify the regulatory mechanisms of PP2A on vascular smooth m…
View article: Highly sensitive myosin phosphorylation analysis in the renal afferent arteriole
Highly sensitive myosin phosphorylation analysis in the renal afferent arteriole Open
The regulation of smooth muscle contraction and relaxation involves phosphorylation and dephosphorylation of regulatory proteins, particularly myosin. To elucidate the regulatory mechanisms, analyzing the phosphorylation signal transductio…