Krishanu Saha
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View article: Longitudinal, Label-Free, High-Resolution Imaging of Glioblastoma Spheroid Response to Therapy: A Translational Tool for Preclinical Evaluation of Chemotherapy, Radiation, and Immunotherapy
Longitudinal, Label-Free, High-Resolution Imaging of Glioblastoma Spheroid Response to Therapy: A Translational Tool for Preclinical Evaluation of Chemotherapy, Radiation, and Immunotherapy Open
View article: Precision therapeutic tRNA rescue of nonsense mutation R166X in KCNJ13 to restore K+ channel function
Precision therapeutic tRNA rescue of nonsense mutation R166X in KCNJ13 to restore K+ channel function Open
View article: Snail Homing and Mating Search Algorithm for Weight Optimization of Stepped-Transmission Shaft
Snail Homing and Mating Search Algorithm for Weight Optimization of Stepped-Transmission Shaft Open
In this paper, the steeped-transmission shaft design problem is proposed for weight optimization. The bio-inspired search-based Snail Homing and Mating Search (SHMS) algorithm is utilized to solve the problem. It is inspired by the social …
View article: Controlling CRISPR-Cas9 genome editing in human cells using a molecular glue degrader
Controlling CRISPR-Cas9 genome editing in human cells using a molecular glue degrader Open
View article: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines Open
Genetic medicines, including CRISPR/Cas technologies, extend tremendous promise for addressing unmet medical need in inherited retinal disorders and other indications; however, there remain challenges for the development of therapeutics. H…
View article: Low-dose radiation by radiopharmaceutical therapy enhances GD2 <i>TRAC</i> -CAR T cell efficacy in localized neuroblastoma
Low-dose radiation by radiopharmaceutical therapy enhances GD2 <i>TRAC</i> -CAR T cell efficacy in localized neuroblastoma Open
Chimeric antigen receptor (CAR) T cells have limited efficacy against solid tumors including neuroblastoma. Here, we evaluated whether low-dose radiation delivered by radiopharmaceutical therapy (RPT), known to potentiate immune checkpoint…
View article: Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy
Longitudinal and continuous label-free monitoring of glioblastoma patient-derived tumor spheroid treatment response using quantitative oblique back illumination microscopy Open
Glioblastoma (GBM) is an aggressive brain tumor with limited treatment options and poor patient survival, underscoring the need for novel therapeutic strategies and improved preclinical models. Patient-derived tumor spheroids (PDTSs) offer…
View article: What does “appropriate scientific justification” mean for the review of human pluripotent stem cell, embryo, and related research?
What does “appropriate scientific justification” mean for the review of human pluripotent stem cell, embryo, and related research? Open
This article complements the 2021 International Society for Stem Cell Research (ISSCR) Guidelines for Stem Cell Research and Clinical Translation by explaining what "adequate and appropriate scientific justification" means for human plurip…
View article: Genome-Wide CRISPR Screening Identifies Cellular Factors Controlling Nonviral Genome Editing Efficiency
Genome-Wide CRISPR Screening Identifies Cellular Factors Controlling Nonviral Genome Editing Efficiency Open
After administering genome editors, their efficiency is limited by a multi-step process involving cellular uptake, trafficking, and nuclear import of the vector and its payload. These processes vary widely across cell types and differ depe…
View article: Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPT
Efficient nonviral integration of large transgenes into human T cells using Cas9-CLIPT Open
View article: Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines
Evaluation of subretinally delivered Cas9 ribonucleoproteins in murine and porcine animal models highlights key considerations for therapeutic translation of genetic medicines Open
Genetic medicines, including CRISPR/Cas technologies, extend tremendous promise for addressing unmet medical need in inherited retinal disorders and other indications; however, there remain challenges for the development of therapeutics. H…
View article: Low Dose Radiation by Radiopharmaceutical Therapy Enhances GD2<i>TRAC-</i>CAR T Cells Efficacy in Localized Neuroblastoma
Low Dose Radiation by Radiopharmaceutical Therapy Enhances GD2<i>TRAC-</i>CAR T Cells Efficacy in Localized Neuroblastoma Open
Background While chimeric antigen receptor (CAR) T cells have achieved significant success against hematological malignancies, efficacy against neuroblastoma has been limited. Virus-free CRISPR-edited GD2 TRAC- CAR T cells have been develo…
View article: Long term rescue of Alzheimer’s deficits <i>in vivo</i> by one-time gene-editing of <i>App</i> C-terminus
Long term rescue of Alzheimer’s deficits <i>in vivo</i> by one-time gene-editing of <i>App</i> C-terminus Open
Gene-editing technologies promise to create a new class of therapeutics that can achieve permanent correction with a single intervention. Besides eliminating mutant alleles in familial disease, gene-editing can also be used to favorably ma…
View article: Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells
Non-viral expression of chimeric antigen receptors with multiplex gene editing in primary T cells Open
Efficient engineering of T cells to express exogenous tumor-targeting receptors such as chimeric antigen receptors (CARs) or T-cell receptors (TCRs) is a key requirement of effective adoptive cell therapy for cancer. Genome editing technol…
View article: Label free metabolic imaging to enhance the efficacy of chimeric antigen receptor T cell therapy
Label free metabolic imaging to enhance the efficacy of chimeric antigen receptor T cell therapy Open
Chimeric antigen receptor (CAR) T cell therapy for solid tumors is challenging not only because of the immunosuppressive tumor microenvironment, but also because of a complex manufacturing process that is difficult to monitor. Manufacturin…
View article: Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence
Metabolic priming of GD2 TRAC-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence Open
Manufacturing chimeric antigen receptor (CAR) T cell therapies is complex, with limited understanding of how medium composition impacts T cell phenotypes. CRISPR-Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting …
View article: Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy
Label free metabolic imaging to enhance the efficacy of Chimeric Antigen Receptor T cell therapy Open
Chimeric antigen receptor (CAR) T cell therapy for solid tumors remains challenging due to the complex manufacturing process and the immunosuppressive tumor microenvironment. The manufacturing condition directly impacts CAR T cell yield, p…
View article: Virus-free CRISPR knock-in of a chimeric antigen receptor into<i>KLRC1</i>generates potent GD2-specific natural killer cells
Virus-free CRISPR knock-in of a chimeric antigen receptor into<i>KLRC1</i>generates potent GD2-specific natural killer cells Open
Natural killer (NK) cells are an appealing off-the-shelf, allogeneic cellular therapy due to their cytotoxic profile. However, their activity against solid tumors remains suboptimal in part due to the upregulation of NK-inhibitory ligands,…
View article: Metabolic priming of GD2<i>TRAC</i>-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence
Metabolic priming of GD2<i>TRAC</i>-CAR T cells during manufacturing promotes memory phenotypes while enhancing persistence Open
Manufacturing Chimeric Antigen Receptor (CAR) T cell therapies is complex, with limited understanding of how media composition impact T-cell phenotypes. CRISPR/Cas9 ribonucleoproteins can precisely insert a CAR sequence while disrupting th…
View article: <i>In vitro</i> encapsulation and expansion of T and CAR-T cells using 3D synthetic thermo-responsive matrices
<i>In vitro</i> encapsulation and expansion of T and CAR-T cells using 3D synthetic thermo-responsive matrices Open
This work employed a biocompatible and synthetic-based thermo-responsive material with tailored mechanical properties as a potential macro-scale scaffold to support ex vivo T and CAR-T cell encapsulation and culture.
View article: Comparative Study of the Effect of Radiation Delivered by Lutetium-177 or Actinium-225 on Anti-GD2 Chimeric Antigen Receptor T Cell Viability and Functions
Comparative Study of the Effect of Radiation Delivered by Lutetium-177 or Actinium-225 on Anti-GD2 Chimeric Antigen Receptor T Cell Viability and Functions Open
Background and purpose. Chimeric antigen receptor (CAR) T cells have been relatively ineffective against solid tumors. Low-dose radiation which can be delivered to multiple sites of metastases by targeted radionuclide therapy (TRT) can eli…
View article: Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells
Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells Open
Genome-edited human-induced pluripotent stem cells (iPSCs) have broad applications in disease modeling, drug discovery, and regenerative medicine. Despite the development of clustered regularly interspaced short palindromic repeats (CRISPR…
View article: Nonviral base editing of KCNJ13 mutation preserves vision in a model of inherited retinal channelopathy
Nonviral base editing of KCNJ13 mutation preserves vision in a model of inherited retinal channelopathy Open
Clinical genome editing is emerging for rare disease treatment, but one of the major limitations is the targeting of CRISPR editors' delivery. We delivered base editors to the retinal pigmented epithelium (RPE) in the mouse eye using silic…
View article: Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors
Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors Open
Introduction: Targeted gene editing is proposed as a therapeutic approach for numerous disorders, including neurological diseases. As the brain is organized into neural networks, it is critical to understand how anatomically connected stru…
View article: Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors
Striatonigral distribution of a fluorescent reporter following intracerebral delivery of genome editors Open
Introduction Targeted gene editing is proposed as a therapeutic approach for numerous disorders, including neurological diseases. As the brain is organized into neural networks, it is critical to understand how anatomically connected struc…
View article: Label-free in vitro assays predict the potency of anti-disialoganglioside chimeric antigen receptor T-cell products
Label-free in vitro assays predict the potency of anti-disialoganglioside chimeric antigen receptor T-cell products Open
View article: Accounting for diversity in the design of CRISPR-based therapeutic genome editing
Accounting for diversity in the design of CRISPR-based therapeutic genome editing Open
View article: Harnessing chimeric antigen receptor (CAR)-T cells as a potential treatment for Alzheimer's disease.
Harnessing chimeric antigen receptor (CAR)-T cells as a potential treatment for Alzheimer's disease. Open
Alzheimer's disease (AD) significantly burdens global healthcare systems given limited treatment options to delay or stop disease progression. Chimeric antigen receptor (CAR) T cell therapy, an immunotherapeutic approach that has produced …
View article: Implementing systems thinking and data science in the training of the regenerative medicine workforce
Implementing systems thinking and data science in the training of the regenerative medicine workforce Open
View article: Efficient in vivo neuronal genome editing in the mouse brain using nanocapsules containing CRISPR-Cas9 ribonucleoproteins
Efficient in vivo neuronal genome editing in the mouse brain using nanocapsules containing CRISPR-Cas9 ribonucleoproteins Open