Kristen M. Wilding
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View article: A consensus mathematical model of vaccine-induced antibody dynamics for multiple vaccine platforms and pathogens
A consensus mathematical model of vaccine-induced antibody dynamics for multiple vaccine platforms and pathogens Open
Introduction Vaccine platforms used in successful, licensed vaccines have varied among pathogens. However, antibody level is still the main clinical correlate of protection in most approved vaccines. Decisions as to the best vaccine platfo…
View article: Evaluating the factors influencing accuracy, interpretability, and reproducibility in the use of machine learning classifiers in biology to enable standardization
Evaluating the factors influencing accuracy, interpretability, and reproducibility in the use of machine learning classifiers in biology to enable standardization Open
The complexity and variability of biological data has promoted the increased use of machine learning methods to understand processes and predict outcomes. These same features complicate reliable, reproducible, interpretable, and responsibl…
View article: Streamlined Production, Protection, and Purification of Enzyme Biocatalysts Using Virus-like Particles and a Cell-Free Protein Synthesis System
Streamlined Production, Protection, and Purification of Enzyme Biocatalysts Using Virus-like Particles and a Cell-Free Protein Synthesis System Open
Enzymes play an essential role in many different industries; however, their operating conditions are limited due to the loss of enzyme activity in the presence of proteases and at temperatures significantly above physiological conditions. …
View article: Quantification of heterogeneity in human CD8+ T cell responses to vaccine antigens: an HLA-guided perspective
Quantification of heterogeneity in human CD8+ T cell responses to vaccine antigens: an HLA-guided perspective Open
Vaccines have historically played a pivotal role in controlling epidemics. Effective vaccines for viruses causing significant human disease, e.g. , Ebola, Lassa fever, or Crimean Congo hemorrhagic fever virus, would be invaluable to public…
View article: Correlating transcription and protein expression profiles of immune biomarkers following lipopolysaccharide exposure in lung epithelial cells
Correlating transcription and protein expression profiles of immune biomarkers following lipopolysaccharide exposure in lung epithelial cells Open
Universal and early recognition of pathogens occurs through recognition of evolutionarily conserved pathogen associated molecular patterns (PAMPs) by innate immune receptors and the consequent secretion of cytokines and chemokines. The int…
View article: Factors Influencing Accuracy, Interpretability and Reproducibility in the use of Machine Learning in Biology
Factors Influencing Accuracy, Interpretability and Reproducibility in the use of Machine Learning in Biology Open
The complexity and variability of biological data has promoted the increased use of machine learning methods to understand processes and predict outcomes. These same features complicate reliable, reproducible, interpretable, and responsibl…
View article: Nonsynonymous amino acid changes in the α-chain of complement component 5 influence longitudinal susceptibility to Plasmodium falciparum infections and severe malarial anemia in kenyan children
Nonsynonymous amino acid changes in the α-chain of complement component 5 influence longitudinal susceptibility to Plasmodium falciparum infections and severe malarial anemia in kenyan children Open
Background: Severe malarial anemia (SMA; Hb < 5.0 g/dl) is a leading cause of childhood morbidity and mortality in holoendemic Plasmodium falciparum transmission regions such as western Kenya. Methods: We investigated the relationship b…
View article: 21-Plex Microfluidic Flow Cytometer and its Potential Applications to Pediatric Malarial Immune Response Analysis
21-Plex Microfluidic Flow Cytometer and its Potential Applications to Pediatric Malarial Immune Response Analysis Open
View article: Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator
Towards On-Demand E. coli-Based Cell-Free Protein Synthesis of Tissue Plasminogen Activator Open
Stroke is the leading cause of death with over 5 million deaths worldwide each year. About 80% of strokes are ischemic strokes caused by blood clots. Tissue plasminogen activator (tPa) is the only FDA-approved drug to treat ischemic stroke…
View article: Non-canonical amino acid labeling in proteomics and biotechnology
Non-canonical amino acid labeling in proteomics and biotechnology Open
Metabolic labeling of proteins with non-canonical amino acids (ncAAs) provides unique bioorthogonal chemical groups during de novo synthesis by taking advantage of both endogenous and heterologous protein synthesis machineries. Labeled pro…
View article: Engineering Cell-Free Biosystems for On-Site Production and Rapid Design of Next-Generation Therapeutics
Engineering Cell-Free Biosystems for On-Site Production and Rapid Design of Next-Generation Therapeutics Open
While protein therapeutics are indispensable in the treatment of a variety of diseases, including cancer, rheumatoid arthritis, and diabetes, key limitations including short half-lives, high immunogenicity, protein instability, and central…
View article: The growing impact of lyophilized cell-free protein expression systems
The growing impact of lyophilized cell-free protein expression systems Open
Recently reported shelf-stable, on-demand protein synthesis platforms are enabling new possibilities in biotherapeutics, biosensing, biocatalysis, and high throughput protein expression. Lyophilized cell-free protein expression systems not…
View article: Rational Engineering of Protein Stability for Potential Application as Detectors in NASA Space Missions
Rational Engineering of Protein Stability for Potential Application as Detectors in NASA Space Missions Open