K. Kilian
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View article: Engineering tissue patterning in human stem cell-based embryo models
Engineering tissue patterning in human stem cell-based embryo models Open
Human embryonic development is challenging to study in vitro as animal models inadequately represent human biology, while use of natural human embryos is both ethically and technically limited. Stem cell-based embryo models (SCBEMs) have e…
View article: A biofabricated 3D cancer-stroma tumor microenvironment model
A biofabricated 3D cancer-stroma tumor microenvironment model Open
Breast cancer progression is a consequence of intricate dynamics between cells and their matrix in the tumor microenvironment. However, most in vitro models are not amenable to studying the behavior of multiple cell types within a defined …
View article: Capillary constrictions prime cancer cell tumorigenicity through PIEZO1
Capillary constrictions prime cancer cell tumorigenicity through PIEZO1 Open
Metastasis is a hallmark of cancer and is responsible for the majority of cancer-related deaths. Evidence suggests that even a single cancer cell can spread and seed a secondary tumour. However, not all circulating tumour cells have this a…
View article: Gene-Corrected Basal Cells Restore CFTR In Vitro; Transplants Regenerate Epithelium in a Preclinical Sinus Model
Gene-Corrected Basal Cells Restore CFTR In Vitro; Transplants Regenerate Epithelium in a Preclinical Sinus Model Open
Background Cystic fibrosis (CF) is caused by mutations in the CFTR gene, leading to epithelial dysfunction and progressive lung disease. Although CFTR modulators have transformed care, ∼10% of people with CF remain without effective therap…
View article: Interfacial Stress Regulates Plasticity and Drug Resistance at the Breast Cancer‐Host Interface
Interfacial Stress Regulates Plasticity and Drug Resistance at the Breast Cancer‐Host Interface Open
The confinement of breast cancer cells at the interface of the mammary gland lumen and its surrounding extracellular matrix is thought to be a key physical driver of cellular plasticity. The relationship between confinement‐induced solid s…
View article: Decoding Hydrogel Porosity: Advancing the Structural Analysis of Hydrogels for Biomedical Applications
Decoding Hydrogel Porosity: Advancing the Structural Analysis of Hydrogels for Biomedical Applications Open
Hydrogels are essential biomaterials for biomedical applications, valued for their tunable properties and biocompatibility. A key feature influencing their function is porosity, which governs transport properties. Cryogenic scanning electr…
View article: Scrambled RGD Hexameric Peptide Hydrogel Supports Efficient Self‐Assembly and Cell Activity
Scrambled RGD Hexameric Peptide Hydrogel Supports Efficient Self‐Assembly and Cell Activity Open
The amino acid sequence is crucial in controlling peptide‐based hydrogel formation, whereby changing the position of a single amino acid can significantly alter the gel's properties. Herein, we report the gelation kinetics and cell viabili…
View article: Stretch activated molecule immobilization in disulfide linked double network hydrogels
Stretch activated molecule immobilization in disulfide linked double network hydrogels Open
Inspired by how forces facilitate molecule immobilization in biological tissues to provide localized functionalization, tough hydrogel networks with stretch activated mechanochemistry are developed by utilizing disulfide bonds as dynamic c…
View article: Magnetoactive Nanotopography on Hydrogels for Stimulated Cell Adhesion and Differentiation
Magnetoactive Nanotopography on Hydrogels for Stimulated Cell Adhesion and Differentiation Open
Advanced cell culture platforms that vary the biophysical microenvironment are useful tools for mechanobiology studies and for directing the differentiation of adherent cells to therapeutically relevant phenotypes. Herein, the fabrication …
View article: Stem cell mechanoadaptation. I. Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling
Stem cell mechanoadaptation. I. Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling Open
Here, we report on the first part of a two-part experimental series to elucidate spatiotemporal cytoskeletal remodeling, which underpins the evolution of stem cell shape and fate, and the emergence of tissue structure and function. In Part…
View article: Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling
Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling Open
Stem cells adapt to their local mechanical environment by rearranging their cytoskeleton, which underpins the evolution of their shape and fate as well as the emergence of tissue structure and function. Here, in the second part of a two-pa…
View article: Direct detection of microRNA in liquid biopsies from single cancer spheroids
Direct detection of microRNA in liquid biopsies from single cancer spheroids Open
The biosensing platform detected miRNA-155 from a single breast cancer spheroid and enabled dynamic tracking of its level changes after anti-cancer treatment, showing the potential to help oncologists develop more personalized treatment st…
View article: Multi-step functionalization of hydrogels through mechano- and photo-responsive linkages
Multi-step functionalization of hydrogels through mechano- and photo-responsive linkages Open
Mechanochemistry can be used to pattern soft materials with cell adhesion motifs, where force and light trigger chemical reactions, thereby facilitating spatial organization of adherent cells.
View article: Tuning mechanical<i>milieux</i>of tissue templates and their cellular inhabitants to guide mechanoadaptation
Tuning mechanical<i>milieux</i>of tissue templates and their cellular inhabitants to guide mechanoadaptation Open
Mechanomics describes the adaptation of mesenchymal stem cells (MSCs) to their mechanical environment, via cytoskeletal remodeling, as well as changes in shape and volume, ultimately resulting in emergent lineage commitment. Here we elucid…
View article: Considerations Influencing Offense-Defense Dynamics From Artificial Intelligence
Considerations Influencing Offense-Defense Dynamics From Artificial Intelligence Open
The rapid advancement of artificial intelligence (AI) technologies presents profound challenges to societal safety. As AI systems become more capable, accessible, and integrated into critical services, the dual nature of their potential is…
View article: Scrambled RGD hexameric peptide hydrogel supports efficient self-assembly and cell activity
Scrambled RGD hexameric peptide hydrogel supports efficient self-assembly and cell activity Open
Amino acid sequence is crucial in controlling peptide-based hydrogel formation, whereby changing the position of a single amino acid can significantly alter the gel’s properties. Herein, we report the gelation kinetics and cell viability o…
View article: Probing the Interplay of Protein Self‐Assembly and Covalent Bond Formation in Photo‐Crosslinked Silk Fibroin Hydrogels
Probing the Interplay of Protein Self‐Assembly and Covalent Bond Formation in Photo‐Crosslinked Silk Fibroin Hydrogels Open
Covalent crosslinking of silk fibroin via native tyrosine residues has been extensively explored; however, while these materials are very promising for biomedical, optical, soft robotics, and sensor applications, their structure and mechan…
View article: Laponite Nanoclay‐Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis
Laponite Nanoclay‐Loaded Microgel Suspensions as Supportive Matrices for Osteogenesis Open
Microscale carriers have emerged as promising materials for nurturing cell growth and as delivery vehicles for regenerative therapies. Carriers based on granular hydrogels have proved advantageous, where “microgels” can be formulated to ha…
View article: Stem cell mechanoadaptation Part B - Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling
Stem cell mechanoadaptation Part B - Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling Open
Stem cells adapt to their local mechanical environment by rearranging their cytoskeleton, which underpins the evolution of their shape and fate, as well as the emergence of tissue structure and function. Here we report on the second part o…
View article: Stem cell mechanoadaptation - Part A - Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling
Stem cell mechanoadaptation - Part A - Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling Open
Here we report on the first part of a two-part experimental series to elucidate spatiotemporal cytoskeletal remodeling, which underpins the evolution of stem cell shape and fate, and the emergence of tissue structure and function. In Part …
View article: Mechanical and biological behavior of double network hydrogels reinforced with alginate versus gellan gum
Mechanical and biological behavior of double network hydrogels reinforced with alginate versus gellan gum Open
Alginate and gellan gum have both been used by researchers as reinforcing networks to create tough and biocompatible polyethylene glycol (PEG) based double network (DN) hydrogels; however, the relative advantages and disadvantages of each …
View article: Advancing Synthetic Hydrogels through Nature‐Inspired Materials Chemistry
Advancing Synthetic Hydrogels through Nature‐Inspired Materials Chemistry Open
Synthetic extracellular matrix (ECM) mimics that can recapitulate the complex biochemical and mechanical nature of native tissues are needed for advanced models of development and disease. Biomedical research has heavily relied on the use …
View article: Biofabrication approaches to fabricating gradients and interfaces in osteochondral tissue engineering
Biofabrication approaches to fabricating gradients and interfaces in osteochondral tissue engineering Open
Osteochondral tissue represents a complex biochemical and biophysical gradient between two distinctly different types of tissue. Its poor regeneration capabilities necessitate tissue engineering intervention; however, its complex structure…
View article: Mesenchymal stem cell‐secretome laden photopolymerizable hydrogels for wound healing
Mesenchymal stem cell‐secretome laden photopolymerizable hydrogels for wound healing Open
Mesenchymal stem cell‐derived secretome represents an emerging acellular therapeutic which possess significant opportunity for clinical applications due to its anti‐inflammatory, immunomodulatory, and wound healing properties. However, mai…
View article: Enzyme Bioink for the 3D Printing of Biocatalytic Materials
Enzyme Bioink for the 3D Printing of Biocatalytic Materials Open
The field of 3D biofabrication faces major challenges on the road to printing fully functional tissues and organs. One of them is adding functionality to the newly formed tissue for replicating an active biochemical environment. Native ext…
View article: Photocrosslinked Silk Fibroin Microgel Scaffolds for Biomedical Applications
Photocrosslinked Silk Fibroin Microgel Scaffolds for Biomedical Applications Open
Silk fibroin hydrogels are extensively explored for tissue engineering and regenerative medicine as an artificial extracellular matrix (ECM) that can support tissue growth. However, the nanometer pore size of hydrogels limits adequate cell…