Kunihiro Okano
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View article: Increased Prorenin Expression in the Kidneys May Be Involved in the Abnormal Renal Function Caused by Prolonged Environmental Exposure to Microcystin-LR
Increased Prorenin Expression in the Kidneys May Be Involved in the Abnormal Renal Function Caused by Prolonged Environmental Exposure to Microcystin-LR Open
Toxic algae in eutrophic lakes produce cyanotoxic microcystins. Prior research on the effect of microcystin-LR in the kidney utilized intraperitoneal injections, which did not reflect natural exposure. Oral microcystin-LR research has focu…
View article: Accelerated manganese(II) removal by in situ mine drainage treatment system without organic substrate amendment: Metagenomic insights into chemolithoautotrophic manganese oxidation via extracellular electron transfer
Accelerated manganese(II) removal by in situ mine drainage treatment system without organic substrate amendment: Metagenomic insights into chemolithoautotrophic manganese oxidation via extracellular electron transfer Open
Mine drainage waters often contain elevated concentrations of toxic metals and can be a source of environmental pollution. Manganese (Mn)-oxidizing bacteria (MnOB), which can oxidize soluble Mn(II) to insoluble Mn(III, IV) under neutral pH…
View article: Effects of Algal Extracellular Polysaccharides on the Formation of Filamentous Manganese Oxide Particles in the Near-Bottom Layer of Lake Biwa
Effects of Algal Extracellular Polysaccharides on the Formation of Filamentous Manganese Oxide Particles in the Near-Bottom Layer of Lake Biwa Open
Filamentous manganese (Mn) oxide particles, which occur in the suboxic zone of stratified waterbodies, are important drivers of diverse elemental cycles. These particles are considered to be bacteriogenic; despite the importance of biogeoc…
View article: Potential contribution of floral thermogenesis to cold adaptation, distribution pattern, and population structure of thermogenic and non/slightly thermogenic <i>Symplocarpus</i> species
Potential contribution of floral thermogenesis to cold adaptation, distribution pattern, and population structure of thermogenic and non/slightly thermogenic <i>Symplocarpus</i> species Open
The genus Symplocarpus in basal Araceae includes both thermogenic and non/slightly thermogenic species that prefer cold environments. If floral thermogenesis of Symplocarpus contributes to cold adaptation, it would be expected that thermog…
View article: Effects of Algal Extracellular Polymeric Substances on the Formation of Filamentous Manganese Oxide Particles in the near-Bottom Layer of Lake Biwa
Effects of Algal Extracellular Polymeric Substances on the Formation of Filamentous Manganese Oxide Particles in the near-Bottom Layer of Lake Biwa Open
Filamentous manganese (Mn) oxide particles, which occur in the suboxic zone of stratified waterbodies, are important drivers of diverse elemental cycles. Although these particles are considered to be bacteriogenic, the environmental factor…
View article: Abundance and Diversity of Nitrifying Microorganisms in Marine Recirculating Aquaculture Systems
Abundance and Diversity of Nitrifying Microorganisms in Marine Recirculating Aquaculture Systems Open
Recirculating aquaculture systems (RAS) are important for water quality management in aquaculture facilities, and can help resume water consumption. However, information about the community structure of the micro-ecosystem existing in biof…
View article: Application of <scp>eDNA</scp> for monitoring freshwater bivalve <i>Nodularia nipponensis</i> and its glochidium larvae
Application of <span>eDNA</span> for monitoring freshwater bivalve <i>Nodularia nipponensis</i> and its glochidium larvae Open
Habitat loss, the introduction of invasive species, and climate change due to human activity have threatened many freshwater unionid bivalves worldwide. Unionid bivalves represent important members of freshwater ecosystems, providing bitte…
View article: Removal of Mn(II) and Zn(II) Ions from Synthetic Mine Drainage Using a Laboratory-Scale Mn(II)-Oxidizing Bioreactor
Removal of Mn(II) and Zn(II) Ions from Synthetic Mine Drainage Using a Laboratory-Scale Mn(II)-Oxidizing Bioreactor Open
鉱山で排出されるマンガン含有坑廃水の生物処理技術の開発を目的として、マンガン酸化細菌を用いたバイオリアクター試験を検討した。マンガン酸化物及びマンガン酸化能を有する細菌群集が付着した石灰石を充填した小型バイオリアクターを構築し、Mn2+イオン単独またはMn2+及びZn2+イオンを含む模擬坑廃水を通水して、これらの金属イオンの負荷がマンガン除去に及ぼす影響を調査した。水理学的滞留時間は24時間に、初期Mn2+濃度は10 mg/Lに設定した。試験の結果、マンガン除去速度はリアク…
View article: Evaluation of Dissolved Greenhouse Gas Profiles in Paddy Fields Irrigated with Treated Sewage Effluent
Evaluation of Dissolved Greenhouse Gas Profiles in Paddy Fields Irrigated with Treated Sewage Effluent Open
As the need for sustainable rice cultivation increases, irrigation with treated sewage effluent (TSE) has become a reasonable solution and its impact on the emission of greenhouse gas (GHG), CH4 and N2O, has gained increasing attention. De…
View article: Concentration Levels and Regional Distributions of Systemic Insecticides and Their Metabolites in the Lake Hachiro Basin, Akita
Concentration Levels and Regional Distributions of Systemic Insecticides and Their Metabolites in the Lake Hachiro Basin, Akita Open
2020年6月と8月に秋田県八郎湖流域における浸透移行性殺虫剤および代謝物の濃度レベルと水平分布の調査を行った。その結果, ジノテフラン, イミダクロプリド, チアクロプリド, チアメトキサム, クロチアニジン, エチプロールとフィプロニルおよび代謝物のチアクロプリドアミドが検出された。最大検出率はジノテフランが100%, チアクロプリドアミドが80%であり, 水稲生産の影響が示唆された。最大検出濃度は, 8月の湖内でジノテフランが2,200 ng L-1, 6月の流入河川…
View article: Microcystin-LR incorporated into colonic cells through probenecid-sensitive transporters leads to upregulated MCP-1 expression induced by JNK activation
Microcystin-LR incorporated into colonic cells through probenecid-sensitive transporters leads to upregulated MCP-1 expression induced by JNK activation Open
View article: Complementary combination of multiplex high‐throughput <scp>DNA</scp> sequencing for molecular phylogeny
Complementary combination of multiplex high‐throughput <span>DNA</span> sequencing for molecular phylogeny Open
The rapid development of DNA sequencing technology in recent years has provided new tools for phylogenetic data acquisition. By using high‐throughput DNA sequencing technology, molecular phylogenetic information can be obtained more quickl…
View article: Peer Review #2 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.1)"
Peer Review #2 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.1)" Open
Increased ungulate browsing alters the composition of plant communities and modifies forest ecosystems worldwide.Ungulates alter their diet following changes in availability of plant species, however, we know little about how browse select…
View article: Peer Review #1 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.1)"
Peer Review #1 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.1)" Open
Increased ungulate browsing alters the composition of plant communities and modifies forest ecosystems worldwide.Ungulates alter their diet following changes in availability of plant species, however, we know little about how browse select…
View article: Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer
Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer Open
Increased ungulate browsing alters the composition of plant communities and modifies forest ecosystems worldwide. Ungulates alter their diet following changes in availability of plant species; however, we know little about how browse selec…
View article: Peer Review #1 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.2)"
Peer Review #1 of "Variability in deer diet and plant vulnerability to browsing among forests with different establishment years of sika deer (v0.2)" Open
Increased ungulate browsing alters the composition of plant communities and modifies forest ecosystems worldwide.Ungulates alter their diet following changes in availability of plant species, however, we know little about how browse select…
View article: Long-term Assessment of N<sub>2</sub>O Emission Factor in Full-scale Oxidation Ditch Reactor Considering Spatiotemporal Distribution
Long-term Assessment of N<sub>2</sub>O Emission Factor in Full-scale Oxidation Ditch Reactor Considering Spatiotemporal Distribution Open
In full-scale sewage treatment plants, long-term and high-frequency monitoring is required to mitigate nitrous oxide (N2O) emissions. In this study, the profile of the dissolved N2O concentration in a full-scale oxidation ditch reactor was…
View article: Assimilation of Cyanobacteria by the Freshwater Bivalve <i>Nodularia douglasiae</i>: Insights from Long-Term Laboratory and Field Feeding Experiments
Assimilation of Cyanobacteria by the Freshwater Bivalve <i>Nodularia douglasiae</i>: Insights from Long-Term Laboratory and Field Feeding Experiments Open
In this study, we conducted long-term laboratory and field feeding experiments to investigate the ability of a freshwater bivalve, Nodularia douglasiae (recently renamed to N. nipponensis) to utilize cyanobacterial cells as food. In the la…
View article: Molecular Cloning and Heterologous Expression of Manganese(II)-Oxidizing Enzyme from Acremonium strictum Strain KR21-2
Molecular Cloning and Heterologous Expression of Manganese(II)-Oxidizing Enzyme from Acremonium strictum Strain KR21-2 Open
Diverse ascomycete fungi oxidize manganese(II) [Mn(II)] and produce Mn(III, IV) oxides in terrestrial and freshwater environments. Although multicopper oxidase (MCO) is considered to be a key catalyst in mediating Mn(II) oxidation in ascom…
View article: Draft Genome Sequence of the Microcystin-Degrading Bacterium <i>Novosphingobium</i> sp. Strain MD-1
Draft Genome Sequence of the Microcystin-Degrading Bacterium <i>Novosphingobium</i> sp. Strain MD-1 Open
This report describes the whole-genome sequence of a microcystin-degrading bacterium, Novosphingobium sp. strain MD-1, isolated from a lake in Japan. The Novosphingobium sp. strain MD-1 genome had a total length of 4,617,766 bp. Moreover, …
View article: EVALUATION OF THE PREDATIOIN AND FEEDING SELECTIVITY ON <i>BOSMINA LONGIROTRIS</i> BY POND SMELT USING IN LAKE HACHIRO
EVALUATION OF THE PREDATIOIN AND FEEDING SELECTIVITY ON <i>BOSMINA LONGIROTRIS</i> BY POND SMELT USING IN LAKE HACHIRO Open
ゾウミジンコが藍藻を餌として利用していることから，ゾウミジンコを活用した藍藻抑制技術の開発が期待されている．本研究ではワカサギのゾウミジンコに対する捕食の有無と選択性の評価を目的として，秋田県八郎湖においてワカサギの胃内容物および脂肪酸組成を調べた．藍藻が異常増殖する夏季に，ワカサギ胃内容物はゾウミジンコに85.4％を占められており，ゾウミジンコが主な餌源であることが分かった．特に，体長5cm以下のワカサギが選択的にゾウミジンコを捕食していた．また，夏季にワカサギとゾウミジ…
View article: Principal Component Analysis applied directly to Sequence Matrix
Principal Component Analysis applied directly to Sequence Matrix Open
Sequence data is now widely used to observe relationships among organisms. However, understanding structure of the qualitative data is challenging. Conventionally, the relationships are analysed using a dendrogram that estimates a tree sha…
View article: Relationship between Diurnal Vertical Migration of Cyanobacteria and the Nutrient Dynamics in a Shallow Eutrophic Lake Shore
Relationship between Diurnal Vertical Migration of Cyanobacteria and the Nutrient Dynamics in a Shallow Eutrophic Lake Shore Open
藍藻は湖沼の水柱を日周鉛直移動することが知られているが、波浪の影響で水塊がよく混合する浅い水域で実態を解析した事例は限られている。また、藍藻の日周鉛直移動と栄養塩類濃度の関係も不明な点が多い。本研究では、藍藻類が集積する極端に浅い沿岸域における藍藻類の日周鉛直移動の実態と栄養塩動態との関係を明らかにすることを目的として、毎年藍藻のブルームが観察されている秋田県八郎湖の水深約60cm程度の沿岸域において調査を行った。観測期間中、優占していた藍藻類はMicrocystis属とO…
View article: EVLUETION OF DIETARY UTILIZATION OF BLOOM-FORMING CYANOBACTERIA BY SMALL-BODIED CRUSTACEAN ZOOPLANKTON USING FATTY ACID ANALYSIS
EVLUETION OF DIETARY UTILIZATION OF BLOOM-FORMING CYANOBACTERIA BY SMALL-BODIED CRUSTACEAN ZOOPLANKTON USING FATTY ACID ANALYSIS Open
アオコの原因となる藍藻は，消費者から餌利用されにくいと考えられているが，実際の富栄養湖における消費者のアオコ餌利用の実態は不明な点が多い．そこで，毎年アオコが発生する富栄養湖である秋田県八郎湖において，ゾウミジンコ（Bosmina longirostris），オナガミジンコ（Diaphanosoma brachyurum）およびカイアシ類成体を対象に，脂肪酸分析を用いて藍藻が餌として同化されているか検証した．夏季のアオコ発生時期において藍藻に多く含まれる脂肪酸である18:2…
View article: Current reproductive situation in a reduced population of juvenile mussels (<i>Unio douglasiae</i>) at a eutrophic lake
Current reproductive situation in a reduced population of juvenile mussels (<i>Unio douglasiae</i>) at a eutrophic lake Open
イシガイ目二枚貝は淡水生態系において物質循環や懸濁物の除去など重要な役割を担っているが，近年，世界的に個体数の減尐が報告されている．とくに，稚貝や若い個体が尐ないという報告があることから，再生産が行われていないことが考えられる．そこで，本研究では若年個体がほとんど見られない八郎湖のイシガイ個体群に注目し，イシガイ母貝の幼生放出の有無と時期，そして宿主魚類を検討することで再生産が行われているか検討した．その結果，イシガイ幼生の放出は6月から8月の間に行われていること，主にヌマ…
View article: EVALUATION OF SAFETY AND THE BREWERY-RICE QUALITY CULTIVATED WITH SEWAGE TREATMENT WATER
EVALUATION OF SAFETY AND THE BREWERY-RICE QUALITY CULTIVATED WITH SEWAGE TREATMENT WATER Open
下水処理水を利用して酒造好適米の栽培試験を行い，玄米品質と安全性を評価した．その結果，酒造好適米に要求される基本的な品質は，粗タンパク質が低かったことを除き，概ね達成することができた．その上で，得られた玄米はカリウム含有量が高く，優れた醸造特性や清酒品質に結びつく可能性が示唆された．また水質浄化能の観点からは，窒素およびリンについて，中干し以前の高負荷条件では10∼20％程度，中干し以降の低負荷条件下では80∼90％程度の除去率が得られた．さらに，土壌においては問題となるレ…
View article: A long-term impact of forest disturbance on spruce seedling regeneration on coarse woody debris
A long-term impact of forest disturbance on spruce seedling regeneration on coarse woody debris Open
Storm disturbance have huge impacts on subalpine forest ecosystems. However, long-term effects of such disturbance on regeneration of subalpine forests are poorly understood. Picea jezoensis var. hondoensis has limited and discontinuous di…
View article: Characteristics of Harmful Algal Blooms during a Low Water Temperature Season in Lake Hachiro
Characteristics of Harmful Algal Blooms during a Low Water Temperature Season in Lake Hachiro Open
In this study, we investigated seasonal changes in chlorophyll a (Chl.a), microcystin, and the 16S rRNA gene-based cyanobacterial community structures using 16S rRNA gene amplicon analysis of the surface water of Lake Hachiro, Akita Prefec…
View article: Effect of Low Temperature Treatment and Incubation Temperature on the Recruitment of Cyanobacteria Cells from the Bottom Sediments Collected at Lake Hachiro, Akita
Effect of Low Temperature Treatment and Incubation Temperature on the Recruitment of Cyanobacteria Cells from the Bottom Sediments Collected at Lake Hachiro, Akita Open
秋田県八郎湖では毎年夏季にアオコが発生しており、秋季にはMicrocystis属やAnabaena属等の藍藻が底質に沈降して越冬している。本研究では秋季に底質に沈降した藍藻類の回帰ポテンシャルを評価するために、2016年11月に湖内8地点より採取した底質試料を用いて、温度条件（10、15、20℃）を変えて回帰試験を行った。その結果、全地点の10日間におけるMicrocystis属回帰量は20℃で増加する傾向にあったが、Anabaena属では20℃における回帰がみられなかった…
View article: Whole-Genome Sequences of Two Manganese(II)-Oxidizing Bacteria, <i>Bosea</i> sp. Strain BIWAKO-01 and Alphaproteobacterium Strain U9-1i
Whole-Genome Sequences of Two Manganese(II)-Oxidizing Bacteria, <i>Bosea</i> sp. Strain BIWAKO-01 and Alphaproteobacterium Strain U9-1i Open
This report describes the whole-genome sequences of two Mn(II)-oxidizing bacteria, filamentous Mn oxide microparticle-forming Bosea sp. strain BIWAKO-01 and alphaproteobacterium strain U9-1i.