Kurt Jungermann
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View article: A Fenton reaction at the endoplasmic reticulum is involved in the redox control of hypoxia-inducible gene expression
A Fenton reaction at the endoplasmic reticulum is involved in the redox control of hypoxia-inducible gene expression Open
It has been proposed that hydroxyl radicals (·OH) generated in a perinuclear iron-dependent Fenton reaction are involved in O 2 -dependent gene expression. Thus, it was the aim of this study to localize the cellular compartment in which th…
View article: Hypoxia-inducible factor-1 and hypoxia response elements mediate the induction of plasminogen activator inhibitor-1 gene expression by insulin in primary rat hepatocytes
Hypoxia-inducible factor-1 and hypoxia response elements mediate the induction of plasminogen activator inhibitor-1 gene expression by insulin in primary rat hepatocytes Open
The expression of the plasminogen activator inhibitor-1(PAI-1) gene is enhanced by insulin both in vivo and in various cell types. Because insulin exerts a number of its biologic activities via the phosphatidylinositol 3-kinase and protein…
View article: Activation of glucokinase gene expression by hepatic nuclear factor 4α in primary hepatocytes
Activation of glucokinase gene expression by hepatic nuclear factor 4α in primary hepatocytes Open
Glucokinase (GK) is a key enzyme for glucose utilization in liver and shows a higher expression in the perivenous zone. In primary rat hepatocytes, the GK gene expression was activated by HNF (hepatic nuclear factor)-4α via the sequence −5…
View article: A role of upstrteam stimulatory factor-2a in regulation of plasminogen activator inhibitor-1 expression
A role of upstrteam stimulatory factor-2a in regulation of plasminogen activator inhibitor-1 expression Open
Експресія інгібітори активатора плазміногена-1 (ІАП-1) стимулюється гіпоксією (8 % О2). За цю стимуляцію відповідає ділянка в промоторі IAП-1 (-175/-159), яка містить елемент, що сприймає гіпоксію (ЕСГ-2). З останнім взаємодіє фактор 1, що…
View article: Anaphylatoxin C5a Actions in Rat Liver: Synergistic Enhancement by C5a of Lipopolysaccharide-Dependent α2-Macroglobulin Gene Expression in Hepatocytes Via IL-6 Release from Kupffer Cells
Anaphylatoxin C5a Actions in Rat Liver: Synergistic Enhancement by C5a of Lipopolysaccharide-Dependent α2-Macroglobulin Gene Expression in Hepatocytes Via IL-6 Release from Kupffer Cells Open
The effects of the anaphylatoxins C5a and C3a on the liver are only poorly characterized in contrast to their well known systemic actions. Recently, it has been demonstrated that the anaphylatoxin C5a enhanced glucose output from hepatocyt…
View article: Normal kinetics of intestinal glucose absorption in the absence of GLUT2: Evidence for a transport pathway requiring glucose phosphorylation and transfer into the endoplasmic reticulum
Normal kinetics of intestinal glucose absorption in the absence of GLUT2: Evidence for a transport pathway requiring glucose phosphorylation and transfer into the endoplasmic reticulum Open
Glucose is absorbed through the intestine by a transepithelial transport system initiated at the apical membrane by the cotransporter SGLT-1; intracellular glucose is then assumed to diffuse across the basolateral membrane through GLUT2. H…
View article: The upstream stimulatory factor-2a inhibits plasminogen activator inhibitor-1 gene expression by binding to a promoter element adjacent to the hypoxia-inducible factor-1 binding site
The upstream stimulatory factor-2a inhibits plasminogen activator inhibitor-1 gene expression by binding to a promoter element adjacent to the hypoxia-inducible factor-1 binding site Open
Plasminogen activator inhibitor-1 (PAI-1) expression is induced by hypoxia (8% O2) via the PAI-1 promoter region −175/−159 containing a hypoxia response element (HRE-2) binding the hypoxia-inducible factor-1 (HIF-1) and an adjacent respons…
View article: Perivenous expression of the mRNA of the three hypoxia-inducible factor α-subunits, HIF1α, HIF2α and HIF3α, in rat liver
Perivenous expression of the mRNA of the three hypoxia-inducible factor α-subunits, HIF1α, HIF2α and HIF3α, in rat liver Open
The cDNAs of three hypoxia-inducible factor (HIF) alpha-subunits were cloned from RNA of primary rat hepatocytes by reverse transcriptase PCR. All three cDNAs encoded functionally active proteins, of 825, 874 and 662 amino acids. After tra…
View article: Physiological oxygen tensions modulate expression of the mdr1b multidrug-resistance gene in primary rat hepatocyte cultures
Physiological oxygen tensions modulate expression of the mdr1b multidrug-resistance gene in primary rat hepatocyte cultures Open
P-Glycoprotein transporters encoded by mdr1 (multidrug resistance) genes mediate extrusion of an array of lipophilic xenobiotics from the cell. In rat liver, mdr transcripts have been shown to be expressed mainly in hepatocytes of the peri…
View article: Impaired stimulation of intestinal glucose absorption by portal insulin via hepatoenteral nerves in chronically ethanol‐intoxicated rats
Impaired stimulation of intestinal glucose absorption by portal insulin via hepatoenteral nerves in chronically ethanol‐intoxicated rats Open
In the isolated, jointly perfused small intestine and liver of rats insulin, infused into the portal vein, induced an increase in intestinal glucose absorption via hepatoenteral cholinergic nerves. The possible loss of function of these ne…
View article: Perivenous localization of insulin receptor protein in rat liver, and regulation of its expression by glucose and oxygen in hepatocyte cultures
Perivenous localization of insulin receptor protein in rat liver, and regulation of its expression by glucose and oxygen in hepatocyte cultures Open
Insulin stimulates glucose utilization in the liver, which occurs mainly in the less aerobic, perivenous, zone. Accordingly, the insulin receptor protein was predominantly expressed in this area, although the insulin receptor mRNA was homo…
View article: Induction of Functional Anaphylatoxin C5a Receptors on Hepatocytes by In Vivo Treatment of Rats with IL-6
Induction of Functional Anaphylatoxin C5a Receptors on Hepatocytes by In Vivo Treatment of Rats with IL-6 Open
In normal rat liver, anaphylatoxin C5a receptors (C5aR) are only expressed by nonparenchymal cells, mainly Kupffer cells and hepatic stellate cells, but not by parenchymal cells, i.e., hepatocytes (HC). Nevertheless, C5a stimulates glucose…
View article: Rat complement factor I: molecular cloning, sequencing and expression in tissues and isolated cells
Rat complement factor I: molecular cloning, sequencing and expression in tissues and isolated cells Open
Summary Factor I (FI) is a regulatory serine protease of the complement system which cleaves three peptide bonds in the α‐chain of C3b and two bonds in the α‐chain of C4b thereby inactivating these proteins. The human protein and the recen…
View article: Increase by Anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells: Direct action of prostaglandins and indirect action of thromboxane A2 on hepatocytes
Increase by Anaphylatoxin C5a of glucose output in perfused rat liver via prostanoids derived from nonparenchymal cells: Direct action of prostaglandins and indirect action of thromboxane A2 on hepatocytes Open
In the perfused rat liver the anaphylatoxin C5a enhanced glucose output, reduced flow, and elevated prostanoid overflow. Because hepatocytes (HCs) do not express C5a receptors, the metabolic C5a actions must be indirect, mediated by e.g. p…
View article: Identification of an oxygen-responsive element in the 5′-flanking sequence of the rat cytosolic phosphoenolpyruvate carboxykinase-1 gene, modulating its glucagon-dependent activation
Identification of an oxygen-responsive element in the 5′-flanking sequence of the rat cytosolic phosphoenolpyruvate carboxykinase-1 gene, modulating its glucagon-dependent activation Open
The glucagon-stimulated transcription of the cytosolic phosphoenolpyruvate carboxykinase-1 (PCK1) gene is mediated by cAMP and positively modulated by oxygen in primary hepatocytes. Rat hepatocytes were transfected with constructs containi…
View article: Acute increase, stimulated by prostaglandin E <sub>2</sub> , in glucose absorption via the sodium dependent glucose transporter-1 in rat intestine
Acute increase, stimulated by prostaglandin E <sub>2</sub> , in glucose absorption via the sodium dependent glucose transporter-1 in rat intestine Open
BACKGROUND/AIMS Acute stimulation by cAMP of the sodium dependent glucose cotransporter SGLT1 has previously been shown. As prostaglandin E 2 (PGE 2 ) increases intracellular cAMP concentrations via its receptor subtypes EP2R and EP4R, it …
View article: Involvement of a local Fenton reaction in the reciprocal modulation by O2 of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase gene and the insulin-dependent activation of the glucokinase gene in rat hepatocytes
Involvement of a local Fenton reaction in the reciprocal modulation by O2 of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase gene and the insulin-dependent activation of the glucokinase gene in rat hepatocytes Open
H2O2 mimicked the action of periportal pO2 in the modulation by O2 of the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase (PCK) gene and the insulin-dependent activation of the glucokinase (GK) gene. H2O2 can be conv…
View article: Transcriptional activation of the haem oxygenase-1 gene by cGMP via a cAMP response element/activator protein-1 element in primary cultures of rat hepatocytes
Transcriptional activation of the haem oxygenase-1 gene by cGMP via a cAMP response element/activator protein-1 element in primary cultures of rat hepatocytes Open
The expression of the rate-limiting enzyme of haem degradation, haem oxygenase-1 (HO-1), can be induced by various stimuli, including lipopolysaccharide, tumour necrosis factor α and NO. The NO signal can be transmitted by cGMP, therefore …
View article: Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene
Human mitochondrial phosphoenolpyruvate carboxykinase 2 gene Open
The mitochodrial (mt) phosphoenolpyruvate carboxykinase 2 (PCK2) gene was isolated by screening a human genomic library with a rat cytosolic (cy) PCK1 cDNA probe comprising sequences from exons 2–9 and by PCR amplification of human genomic…
View article: Periportal localization of glucagon receptor mRNA in rat liver and regulation of its expression by glucose and oxygen in hepatocyte cultures
Periportal localization of glucagon receptor mRNA in rat liver and regulation of its expression by glucose and oxygen in hepatocyte cultures Open
Glucagon is the major hormone activating glycogenolysis and gluconeogenesis both localized in the periportal, more aerobic zone of the liver. Accordingly, the glucagon receptor (GcgR) mRNA was found to be predominantly expressed in this ar…
View article: Purification of a RNA-binding Protein from Rat Liver
Purification of a RNA-binding Protein from Rat Liver Open
In cultured rat hepatocytes the degradation of phosphoenolpyruvate carboxykinase mRNA might be regulated by protein(s), which by binding to the mRNA alter its stability. The 3'-untranslated region of phosphoenolpyruvate carboxykinase mRNA …
View article: A new role for enteric glucagon‐37: acute stimulation of glucose absorption in rat small intestine
A new role for enteric glucagon‐37: acute stimulation of glucose absorption in rat small intestine Open
Glucagon‐37 is secreted by intestinal L‐cells following carbohydrate uptake. It is known to inhibit gastric acid secretion (hence also named oxyntomodulin) and appears to increase intracellular cyclic AMP concentrations. Since cyclic AMP c…
View article: Anaphylatoxin C5a receptor mRNA is strongly expressed in Kupffer and stellate cells and weakly in sinusoidal endothelial cells but not in hepatocytes of normal rat liver
Anaphylatoxin C5a receptor mRNA is strongly expressed in Kupffer and stellate cells and weakly in sinusoidal endothelial cells but not in hepatocytes of normal rat liver Open
Anaphylatoxins (C5a and C3a), which are generated during complement activation, have recently been shown to increase glucose output from hepatocytes (HC) in perfused rat liver. They did not act directly on HC but indirectly by prostanoid r…
View article: Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures: mimicry of arterial oxygen pressures by H2O2
Arterial oxygen partial pressures reduce the insulin-dependent induction of the perivenously located glucokinase in rat hepatocyte cultures: mimicry of arterial oxygen pressures by H2O2 Open
Liver glucokinase (GK) is localized predominantly in the perivenous zone. GK mRNA was induced by insulin maximally under venous O2 partial pressure (pO2) and only half-maximally under arterial pO2. CoCl2 and desferrioxamine mimicked venous…
View article: Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice.
Defective propagation of signals generated by sympathetic nerve stimulation in the liver of connexin32-deficient mice. Open
The gap junctional protein connexin32 is expressed in hepatocytes, exocrine pancreatic cells, Schwann cells, and other cell types. We have inactivated the connexin32 gene by homologous recombination in the mouse genome and have generated h…
View article: Inhibition of Anaphylatoxin C3a– and C5a– But Not Nerve Stimulation– or Noradrenaline–Dependent Increase in Glucose Output and Reduction of Flow in Kupffer Cell–Depleted Perfused Rat Livers
Inhibition of Anaphylatoxin C3a– and C5a– But Not Nerve Stimulation– or Noradrenaline–Dependent Increase in Glucose Output and Reduction of Flow in Kupffer Cell–Depleted Perfused Rat Livers Open
In isolated in situ perfused rat livers, infusion of anaphylatoxins C3a and C5a, activation peptides of the complement system, as well as stimulation of sympathetic hepatic nerves have been shown to increase hepatic glucose output and to r…