Kwon-Kyoo Kang
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View article: Loss of LsSOC1 Function Delays Bolting and Reprograms Transcriptional and Metabolic Responses in Lettuce
Loss of LsSOC1 Function Delays Bolting and Reprograms Transcriptional and Metabolic Responses in Lettuce Open
Background/Objectives: Bolting in lettuce (Lactuca sativa L.) is highly sensitive to elevated temperatures, leading to premature flowering and reduced crop quality and yield. Although SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) is a …
View article: Persistence of Phytoplasma and Control Efficacy of Oxytetracycline Tree Injection for Elaeocarpus sylvestris (Lour.) Poir. var. ellipticus (Thunb.) H.Hara Decline Disease
Persistence of Phytoplasma and Control Efficacy of Oxytetracycline Tree Injection for Elaeocarpus sylvestris (Lour.) Poir. var. ellipticus (Thunb.) H.Hara Decline Disease Open
Elaeocarpus sylvestris (Lour.) Poir. var. ellipticus (Thunb.) H.Hara, an evergreen tree species native to Jeju Island, South Korea, has experienced a progressive decline associated with phytoplasma infection. This study aimed to evaluate t…
View article: CRISPR/Cas9-Mediated Knockout of OsbZIP76 Reveals Its Role in ABA-Associated Immune Signaling in Rice
CRISPR/Cas9-Mediated Knockout of OsbZIP76 Reveals Its Role in ABA-Associated Immune Signaling in Rice Open
The basic leucine zipper (bZIP) transcription factors are involved in a wide range of physiological processes in plants, including hormone signaling, stress responses, and growth and development regulation. They play a key role in abscisic…
View article: Transcriptomic Insights into GABA Accumulation in Tomato via CRISPR/Cas9-Based Editing of SlGAD2 and SlGAD3
Transcriptomic Insights into GABA Accumulation in Tomato via CRISPR/Cas9-Based Editing of SlGAD2 and SlGAD3 Open
Background: γ-Aminobutyric acid (GABA) is a non-proteinogenic amino acid with key roles in plant metabolism, stress responses, and fruit nutritional quality. In tomato (Solanum lycopersicum), GABA levels are dynamically regulated during fr…
View article: Effects of dietary inclusion of blooming Ulva sp. on milk production, methane emitting potential, and physiological parameters in lactating Holstein cows
Effects of dietary inclusion of blooming Ulva sp. on milk production, methane emitting potential, and physiological parameters in lactating Holstein cows Open
Objective: Ulva sp., a blooming macroalgae causing the green tide in Korea, has been suggested as a feed ingredient for ruminant livestock. The objective of this study was to investigate the effects of 3% dry matter inclusion of Ulva sp. i…
View article: Vogesella oryzagri sp. nov., isolated from the rhizosphere of rice and in silico genome mining for the prediction of biosynthetic gene clusters
Vogesella oryzagri sp. nov., isolated from the rhizosphere of rice and in silico genome mining for the prediction of biosynthetic gene clusters Open
A Gram-stain-negative, aerobic, rod-shaped, motile and flagellated novel bacterial strain, designated MAHUQ-64 T , was isolated from the rhizosphere of rice. The colonies were observed to be creamy white-coloured, smooth, spherical and 0.5…
View article: Integrative transcriptogenomic analyses reveal the regulatory network underlying rice eating and cooking quality and identify a role for alpha-globulin in modulating starch and sucrose metabolism
Integrative transcriptogenomic analyses reveal the regulatory network underlying rice eating and cooking quality and identify a role for alpha-globulin in modulating starch and sucrose metabolism Open
Rice eating and cooking quality (ECQ) is significantly influenced by the physicochemical properties of rice starch. This study integrates whole-genome resequencing, transcriptomic data, and phenotypic analysis to identify the genetic facto…
View article: ABA and Pre-Harvest Sprouting Differences in Knockout Lines of OsPHS3 Encoding Carotenoid Isomerase via CRISPR/Cas9 in Rice
ABA and Pre-Harvest Sprouting Differences in Knockout Lines of OsPHS3 Encoding Carotenoid Isomerase via CRISPR/Cas9 in Rice Open
We generated and characterized knockout mutant lines of the OsPHS3 gene using the CRISPR/Cas9 system. The knockout lines of the OsPHS3 gene showed that 1 bp and 7 bp deletion, early termination codons were used for protein production. Agro…
View article: Genetic Research and Plant Breeding 2.0
Genetic Research and Plant Breeding 2.0 Open
Recent advances in next-generation sequencing technologies have significantly reduced sequencing costs, resulting in the creation of large-scale genomic data that can be utilized for plant breeding [...]
View article: Knockout of OsGAPDHC7 Gene Encoding Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Affects Energy Metabolism in Rice Seeds
Knockout of OsGAPDHC7 Gene Encoding Cytosolic Glyceraldehyde-3-Phosphate Dehydrogenase Affects Energy Metabolism in Rice Seeds Open
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a major glycolytic enzyme that plays an important role in several cellular processes, including plant hormone signaling, plant development, and transcriptional regulation. In this study, …
View article: The First Domesticated ‘Cheongju Sorori Rice’ Excavated in Korea
The First Domesticated ‘Cheongju Sorori Rice’ Excavated in Korea Open
Archaeological excavations led by Yung-jo Lee and Jong-yoon Woo were carried out twice at the Sorori paleolithic site, Cheongju, in the Republic of Korea, at the upper stream of the Geumgang river, the Miho riverside. A total of 127 rice s…
View article: OsPUB9 Gene Edited by CRISPR/Cas9 Enhanced Resistance to Bacterial Leaf Blight in Rice (Oryza sativa L.)
OsPUB9 Gene Edited by CRISPR/Cas9 Enhanced Resistance to Bacterial Leaf Blight in Rice (Oryza sativa L.) Open
Ubiquitination plays a crucial role in regulating signal pathways during the post-translation stage of protein synthesis in response to various environmental stresses. E3 ubiquitin ligase has been discovered to ultimately control various i…
View article: Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited Tomato SGR1 Knockout (KO) Line
Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited Tomato SGR1 Knockout (KO) Line Open
Tomatoes contain many secondary metabolites such as β-carotene, lycopene, phenols, flavonoids, and vitamin C, which are responsible for antioxidant activity. SlSGR1 encodes a STAY-GREEN protein that plays a critical role in the regulation …
View article: Repeatability of feed efficiency and its relationship with carcass traits in Hanwoo steers during their entire growing and fattening period
Repeatability of feed efficiency and its relationship with carcass traits in Hanwoo steers during their entire growing and fattening period Open
Objective: This study investigated the repeatability of feed efficiency and its association with carcass traits in Hanwoo steers during the entire growing and fattening periods.Methods: The growth and intake of thirty-six Hanwoo steers (25…
View article: Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice
Phenotypic characterization of pre-harvest sprouting resistance mutants generated by the CRISPR/Cas9-geminiviral replicon system in rice Open
Pre-harvest sprouting is a critical phenomenon involving germination of seeds in the mother plant before harvest under relative humid conditions and reduced dormancy. In this paper, we generated HDR mutant lines with one region SNP (C/T) a…
View article: Effect of the Meal Interval Setting of an Automated Concentrate Feeding System on Feed Intake and Feeding Behavior in Fattening Hanwoo Steers
Effect of the Meal Interval Setting of an Automated Concentrate Feeding System on Feed Intake and Feeding Behavior in Fattening Hanwoo Steers Open
An automatic concentrate feeding system (ACFS) divides the day into several intervals, allowing cattle to consume a predetermined amount of concentrate mix per interval. This study investigated the impact of changing these intervals (four …
View article: Effects of Dietary Fat Level of Concentrate Mix on Growth Performance, Rumen Characteristics, Digestibility, Blood Metabolites, and Methane Emission in Growing Hanwoo Steers
Effects of Dietary Fat Level of Concentrate Mix on Growth Performance, Rumen Characteristics, Digestibility, Blood Metabolites, and Methane Emission in Growing Hanwoo Steers Open
This study investigated the effect of different dietary fat levels in concentrate mixes on the growth performance, rumen characteristics, digestibility, blood metabolites, and methane emissions in growing Hanwoo steers. Thirty steers (386 …
View article: Genetic Analysis Based on CRISPR/Cas9 Technology in Plants
Genetic Analysis Based on CRISPR/Cas9 Technology in Plants Open
Genome-editing technology is a type of genetic engineering in which DNA is inserted into, replaced in, or deleted from the genome using artificially engineered nucleases or genetic scissors [...]
View article: Anti-<i>Helicobacter pylori</i> Activity of Six Major Compounds Isolated from <i>Rumex acetosa</i>
Anti-<i>Helicobacter pylori</i> Activity of Six Major Compounds Isolated from <i>Rumex acetosa</i> Open
Gastric problems are often caused by the well-known Helicobacter pylori (H. pylori) bacterium. One of the biggest obstacles to the treatment of H. pylori infections is increasing the antibiotic resistance. During our search for naturally d…
View article: Knockout Mutants of OsPUB7 Generated Using CRISPR/Cas9 Revealed Abiotic Stress Tolerance in Rice
Knockout Mutants of OsPUB7 Generated Using CRISPR/Cas9 Revealed Abiotic Stress Tolerance in Rice Open
Plants produce and accumulate stress-resistant substances when exposed to abiotic stress, which involves a protein conversion mechanism that breaks down stress-damaged proteins and supplies usable amino acids. Eukaryotic protein turnover i…
View article: Genetic Research and Plant Breeding
Genetic Research and Plant Breeding Open
In the past 20 years, plant genetics and breeding research using molecular biology has been greatly improved via the functional analysis of genes, species identification and transformation techniques [...]
View article: Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Gene
Biochemical Characterization of Orange-Colored Rice Calli Induced by Target Mutagenesis of OsOr Gene Open
We generated an orange-colored (OC) rice callus line by targeted mutagenesis of the orange gene (OsOr) using the CRISPR-Cas9 system. The OC line accumulated more lutein, β-carotene, and two β-carotene isomers compared to the WT callus line…
View article: Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System
Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System Open
Stay-green 1 (SGR1) protein is a critical regulator of chlorophyll degradation and senescence in plant leaves; however, the functions of tomato SGR1 remain ambiguous. Here, we generated an SGR1-knockout (KO) null line via clustered regular…
View article: Antioxidant Constituents and Activities of the Pulp with Skin of Korean Tomato Cultivars
Antioxidant Constituents and Activities of the Pulp with Skin of Korean Tomato Cultivars Open
Tomato is a widely distributed, cultivated, and commercialized vegetable crop. It contains antioxidant constituents including lycopene, tocopherols, vitamin C, γ-aminobutyric acid, phenols, and flavonoids. This study determined the content…
View article: Development of SNP Markers from GWAS for Selecting Seed Coat and Aleurone Layers in Brown Rice (Oryza sativa L.)
Development of SNP Markers from GWAS for Selecting Seed Coat and Aleurone Layers in Brown Rice (Oryza sativa L.) Open
Ninety-five percent of the general nutrients in rice are concentrated in the rice bran and germ, and many nutrients such as vitamins, minerals, dietary fiber, and essential fatty acids, as well as antioxidants such as tocopherol, are lost …
View article: Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice
Genome Editing of Golden SNP-Carrying Lycopene Epsilon-Cyclase (LcyE) Gene Using the CRSPR-Cas9/HDR and Geminiviral Replicon System in Rice Open
Lycopene epsilon-cyclase (LcyE) is a key enzyme in the carotenoid biosynthetic pathway of higher plants. Using the CRSPR/Cas9 and the geminiviral replicon, we optimized a method for targeted mutagenesis and golden SNP replacement of the Lc…
View article: Interspecific and Intraspecific Hybrid Rootstocks to Improve Horticultural Traits and Soil-Borne Disease Resistance in Tomato
Interspecific and Intraspecific Hybrid Rootstocks to Improve Horticultural Traits and Soil-Borne Disease Resistance in Tomato Open
Tomato rootstocks are important to increase yield and control soil-borne pathogens, increasing vigor for a longer crop cycle and tolerance to biotic and abiotic stress. This study, conducted in the greenhouse of Sunchon National University…
View article: Rice Genetics: Trends and Challenges for the Future Crops Production
Rice Genetics: Trends and Challenges for the Future Crops Production Open
Twenty-first-century agriculture faces serious challenges in every country on the planet due to global population growth, declining genetic resources, climate change, farmland loss due to urbanization, and stagnant crop yields [...]
View article: Marker-Assisted Backcrossing (MABc) to Improve Eating Quality with Thin Seed Coat and Aleurone Layer of Non-Glutinous Japonica Variety in Rice
Marker-Assisted Backcrossing (MABc) to Improve Eating Quality with Thin Seed Coat and Aleurone Layer of Non-Glutinous Japonica Variety in Rice Open
Brown rice is composed of rice bran, pericarp, seed coat, and aleurone layers, and the rice bran layer contains a large number of substances useful for the human body, such as dietary fiber, α-tocopherol, α-tocotrienol, and vitamins. Howev…