Kyle A. Brown
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View article: Tetrazole-Based Probe: A New Frontier for Scalable, Selective Peroxynitrite Sensing in Cells
Tetrazole-Based Probe: A New Frontier for Scalable, Selective Peroxynitrite Sensing in Cells Open
Peroxynitrite (ONOO−), a highly reactive oxygen and nitrogen species produced during cellular oxidative stress, has emerged as a key tar-get for responsive fluorescent probes. The existing toolbox of responsive chemistries, which predomina…
View article: Polymer Platform for Peroxynitrite Detection Utilizing Benzopyrylium Chemistry
Polymer Platform for Peroxynitrite Detection Utilizing Benzopyrylium Chemistry Open
Continuous detection of peroxynitrite, a highly reactive oxygen and nitrogen species linked to the onset and severity of oxidative stress, is essential for real-time monitoring of disease progression. While benzopyrylium (BZP)-based two-ph…
View article: Top-down mass spectrometry of native proteoforms and their complexes: a community study
Top-down mass spectrometry of native proteoforms and their complexes: a community study Open
View article: Global Proteomic Analysis Reveals Alterations in Differentially Expressed Proteins between Cardiopathic Lamin A/C Mutations
Global Proteomic Analysis Reveals Alterations in Differentially Expressed Proteins between Cardiopathic Lamin A/C Mutations Open
Lamin A/C (LMNA) is an important component of nuclear lamina. Mutations cause arrhythmia, heart failure, and sudden cardiac death. While LMNA-associated cardiomyopathy typically has an aggressive course that responds poorly to conventional…
View article: Editorial Board
Editorial Board Open
View article: Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS)
Phosphorylation Sites of the Gastric Inhibitory Polypeptide Receptor (GIPR) Revealed by Trapped-Ion-Mobility Spectrometry Coupled to Time-of-Flight Mass Spectrometry (TIMS-TOF MS) Open
The gastric inhibitory polypeptide receptor (GIPR), a G protein-coupled receptor (GPCR) that regulates glucose metabolism and insulin secretion, is a target for the development of therapeutic agents to address type 2 diabetes and obesity. …
View article: Exploratory Data Analysis on the RHIC Cryogenics System Compressor Dataset
Exploratory Data Analysis on the RHIC Cryogenics System Compressor Dataset Open
The Relativistic Heavy Ion Collider (RHIC) Cryogenic Refrigerator System is the cryogenic heart that allows RHIC superconducting magnets to operate. Parts of the refrigerator are two stages of compression composed of ten first and five sec…
View article: Effects of Replacing a Central Glycine Residue in GLP‐1 on Receptor Affinity and Signaling Profile
Effects of Replacing a Central Glycine Residue in GLP‐1 on Receptor Affinity and Signaling Profile Open
Agonists of the glucagon‐like peptide‐1 receptor (GLP‐1R) are used to treat diabetes and obesity. Cryo‐EM structures indicate that GLP‐1 is completely α‐helical when bound to the GLP‐1R. The mature form of this hormone, GLP‐1(7‐36), contai…
View article: Top-down mass spectrometry of native proteoforms and their complexes: A community study
Top-down mass spectrometry of native proteoforms and their complexes: A community study Open
The combination of native electrospray ionisation with top-down fragmentation in mass spectrometry allows simultaneous determination of the stoichiometry of noncovalent complexes and identification of their component proteoforms and co-fac…
View article: Comprehensive Characterization of Endogenous Phospholamban Proteoforms Enabled by Photocleavable Surfactant and Top-down Proteomics
Comprehensive Characterization of Endogenous Phospholamban Proteoforms Enabled by Photocleavable Surfactant and Top-down Proteomics Open
Top-down mass spectrometry (MS)-based proteomics has become a powerful tool for analyzing intact proteins and their associated post-translational modifications (PTMs). In particular, membrane proteins play critical roles in cellular functi…
View article: Editorial Board
Editorial Board Open
View article: Editorial Board
Editorial Board Open
View article: Editorial Board
Editorial Board Open
View article: MASH Native: a unified solution for native top-down proteomics data processing
MASH Native: a unified solution for native top-down proteomics data processing Open
Motivation Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Desp…
View article: Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection
Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection Open
Generating top-down tandem mass spectra (MS/MS) from complex mixtures of proteoforms benefits from improvements in fractionation, separation, fragmentation, and mass analysis. The algorithms to match MS/MS to sequences have undergone a par…
View article: High sensitivity top–down proteomics captures single muscle cell heterogeneity in large proteoforms
High sensitivity top–down proteomics captures single muscle cell heterogeneity in large proteoforms Open
Single-cell proteomics has emerged as a powerful method to characterize cellular phenotypic heterogeneity and the cell-specific functional networks underlying biological processes. However, significant challenges remain in single-cell prot…
View article: Editorial Board
Editorial Board Open
View article: Comprehensive Characterization of Endogenous Phospholamban Proteoforms Enabled by Photocleavable Surfactant and Top-down Proteomics
Comprehensive Characterization of Endogenous Phospholamban Proteoforms Enabled by Photocleavable Surfactant and Top-down Proteomics Open
Top-down mass spectrometry (MS)-based proteomics has become a powerful tool for analyzing intact proteins and their associated post-translational modification (PTMs). In particular, membrane proteins play critical roles in cellular functio…
View article: Editorial Board
Editorial Board Open
View article: Synthesis, Self-Assembly Properties, and Degradation Characterization of a Nonionic Photocleavable Azo-Sulfide Surfactant Family
Synthesis, Self-Assembly Properties, and Degradation Characterization of a Nonionic Photocleavable Azo-Sulfide Surfactant Family Open
We report the synthesis and characterization of a new family of maltose-derived nonionic surfactants that contain a photocleavable azo-sulfide linker (mAzo). The self-assembly properties of these surfactants were investigated using surface…
View article: Nonionic, Cleavable Surfactant for Top-Down Proteomics
Nonionic, Cleavable Surfactant for Top-Down Proteomics Open
Nonionic surfactants are often used as general reagents for cell lysis enabling protein extraction, stabilization, and purification under nondenaturing conditions for downstream analysis in structural biology. However, the presence of surf…
View article: MASH Native: A Unified Solution for Native Top-Down Proteomics Data Processing
MASH Native: A Unified Solution for Native Top-Down Proteomics Data Processing Open
Native top-down proteomics (nTDP) integrates native mass spectrometry (nMS) with top-down proteomics (TDP) to provide comprehensive analysis of protein complexes together with proteoform identification and characterization. Despite signifi…
View article: High Sensitivity Top-down Proteomics Captures Single Muscle Cell Heterogeneity in Large Proteoforms
High Sensitivity Top-down Proteomics Captures Single Muscle Cell Heterogeneity in Large Proteoforms Open
Single-cell proteomics has emerged as a powerful method to characterize cellular phenotypic heterogeneity and the cell-specific functional networks underlying biological processes. However, significant challenges remain in single-cell prot…
View article: Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection
Comparing Top-Down Proteoform Identification: Deconvolution, PrSM Overlap, and PTM Detection Open
Generating top-down tandem mass spectra (MS/MS) for complex mixtures of proteoforms has become possible through improvements in fractionation, on-line separation, dissociation, and mass analysis. The algorithms to match MS/MS to sequences …
View article: Necroptosis is associated with Rab27‐independent expulsion of extracellular vesicles containing RIPK3 and MLKL
Necroptosis is associated with Rab27‐independent expulsion of extracellular vesicles containing RIPK3 and MLKL Open
Extracellular vesicle (EV) secretion is an important mechanism used by cells to release biomolecules. A common necroptosis effector—mixed lineage kinase domain like (MLKL)—was recently found to participate in the biogenesis of small and la…
View article: One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant
One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant Open
Exosomes are small extracellular vesicles (EVs) secreted by all cells and found in biological fluids, which can serve as minimally invasive liquid biopsies with extremely high therapeutic and diagnostic potential. Mass spectrometry (MS)-ba…
View article: One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant
One-Pot Exosome Proteomics Enabled by a Photocleavable Surfactant Open
Exosomes are small extracellular vesicles (EVs) secreted by all cells and found in biological fluids, which can serve as minimally invasive liquid biopsies with high therapeutic and diagnostic potential. Mass spectrometry (MS)-based proteo…
View article: Proteomic Analysis of the Functional Inward Rectifier Potassium Channel (Kir) 2.1 Reveals Several Novel Phosphorylation Sites
Proteomic Analysis of the Functional Inward Rectifier Potassium Channel (Kir) 2.1 Reveals Several Novel Phosphorylation Sites Open
Membrane proteins represent a large family of proteins that perform vital physiological roles and represent key drug targets. Despite their importance, bioanalytical methods aiming to comprehensively characterize the post-translational mod…
View article: Ultrafast and Reproducible Proteomics from Small Amounts of Heart Tissue Enabled by Azo and timsTOF Pro
Ultrafast and Reproducible Proteomics from Small Amounts of Heart Tissue Enabled by Azo and timsTOF Pro Open
Global bottom-up mass spectrometry (MS)-based proteomics is widely used for protein identification and quantification to achieve a comprehensive understanding of the composition, structure, and function of the proteome. However, traditiona…
View article: Synthesis of Extended Uridine Phosphonates Derived from an Allosteric P2Y2 Receptor Ligand
Synthesis of Extended Uridine Phosphonates Derived from an Allosteric P2Y2 Receptor Ligand Open
In this study we report the synthesis of C5/C6-fused uridine phosphonates that are structurally related to earlier reported allosteric P2Y2 receptor ligands. A silyl-Hilbert-Johnson reaction of six quinazoline-2,4-(1H,3H)-dione-like base m…