Kyle Hoffman
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View article: Supplementary Table S4 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Table S4 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Antibodies used for flow cytometry staining
View article: Supplementary Table S5 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Table S5 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Antibodies used for immunohistochemistry staining
View article: Supplementary Figure S5 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S5 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Rocaglates inhibit NRF2 translation via cap-dependent initiation, supported by CCLE correlation, immunoblots, luciferase assays, and siRNA knockdown.
View article: Supplementary Table S2 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Table S2 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Western blotting antibodies
View article: Data from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Data from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Cancers co-opt stress response pathways to drive oncogenesis, dodge immune surveillance, and resist cytotoxic therapies. Several of these pathways also provide protection from ferroptosis, an iron-dependent oxidative cell death pathway tri…
View article: Supplementary Table S3 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Table S3 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Primers used for qPCR
View article: Supplementary Figure S3 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S3 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Zotatifin-induced oxidative stress and ferroptosis in DLBCL cells assessed by BODIPY oxidation, GSH/GSSG ratio, MitoSOX, and DHE staining.
View article: Supplementary Figure S7 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S7 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Tumor imaging and immunohistochemistry analysis of cleaved caspase 3 and 4-HNE in vivo following zotatifin, IKE, or combination treatment.
View article: Supplementary Figure S2 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S2 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Integrated analysis of translation and transcription upon zotatifin treatment, including immunoblots, TMT-pSILAC data, and GSEA enrichment.
View article: Supplementary Figure S4 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S4 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Immunoblot analysis of apoptosis and ferroptosis markers in DLBCL cells treated with zotatifin in combination with RSL3 or DMF.
View article: Supplementary Figure S1 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S1 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Transcriptomic analysis of zotatifin-treated DLBCL cells including RNA-seq heatmap, GSEA pathway enrichment, and NF-κB signaling readouts.
View article: Supplementary Figure S8 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S8 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
CAR T cell co-culture assays and in vivo tumor growth studies assessing the synergy of zotatifin with CAR T immunotherapy.
View article: Supplementary Figure S6 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Figure S6 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Zotatifin sensitizes cells to ferroptosis; includes lipid peroxidation, ROS analysis, and Bliss synergy plots.
View article: Supplementary Table S1 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Supplementary Table S1 from Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
List of reagents
View article: Covalent Ligand Electrophiles Are Differentially Activated by Proximity Effects Which Govern Latent Protein Reactivity
Covalent Ligand Electrophiles Are Differentially Activated by Proximity Effects Which Govern Latent Protein Reactivity Open
Covalent ligands contain an electrophilic moiety that reacts with a nucleophilic residue on a target protein, following an initial reversible binding event. Covalent ligand development typically involves efforts to increase on-target selec…
View article: Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy
Blocking NRF2 Translation by Inhibition of Cap-Dependent Initiation Sensitizes Lymphoma Cells to Ferroptosis and CAR T-cell Immunotherapy Open
Cancers co-opt stress response pathways to drive oncogenesis, dodge immune surveillance, and resist cytotoxic therapies. Several of these pathways also provide protection from ferroptosis, an iron-dependent oxidative cell death pathway tri…
View article: High-fidelity and differential nonsense suppression in live cells and a frontotemporal dementia allele with human transfer RNAs
High-fidelity and differential nonsense suppression in live cells and a frontotemporal dementia allele with human transfer RNAs Open
Nonsense mutations generate premature termination codons (PTCs) that are responsible for 11% of genetic disease alleles. The arginine (Arg, CGA) to stop (UGA) mutation is the most common PTC. Humans encode >600 transfer RNA (tRNA) genes…
View article: Transfer RNA supplementation rescues HARS deficiency in a humanized yeast model of Charcot-Marie-Tooth disease
Transfer RNA supplementation rescues HARS deficiency in a humanized yeast model of Charcot-Marie-Tooth disease Open
Aminoacyl-tRNA synthetases are indispensable enzymes in all cells, ensuring the correct pairing of amino acids to their cognate tRNAs to maintain translation fidelity. Autosomal dominant mutations V133F and Y330C in histidyl-tRNA synthetas…
View article: NRF2 translation block by inhibition of cap-dependent initiation sensitizes lymphoma cells to ferroptosis and CAR-T immunotherapy
NRF2 translation block by inhibition of cap-dependent initiation sensitizes lymphoma cells to ferroptosis and CAR-T immunotherapy Open
Cancers coopt stress-response pathways to drive oncogenesis, dodge immune surveillance, and resist cytotoxic therapies. Several of these provide protection from ferroptosis, iron-mediated oxidative cell death. Here, we found dramatic sensi…
View article: Mistranslating the genetic code with leucine in yeast and mammalian cells
Mistranslating the genetic code with leucine in yeast and mammalian cells Open
Translation fidelity relies on accurate aminoacylation of transfer RNAs (tRNAs) by aminoacyl-tRNA synthetases (AARSs). AARSs specific for alanine (Ala), leucine (Leu), serine, and pyrrolysine do not recognize the anticodon bases. Single nu…
View article: Raw LC-MS/MS data files for yeast Leu mistranslation
Raw LC-MS/MS data files for yeast Leu mistranslation Open
This research identifies and quantifies mistranslation of phenylalanine codons with leucine across the Saccharomyces cerevisiae proteome.
View article: Recoding UAG to selenocysteine in<i>Saccharomyces cerevisiae</i>
Recoding UAG to selenocysteine in<i>Saccharomyces cerevisiae</i> Open
Unique chemical and physical properties are introduced by inserting selenocysteine (Sec) at specific sites within proteins. Recombinant and facile production of eukaryotic selenoproteins would benefit from a yeast expression system; howeve…
View article: Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves
Experimental Senecavirus A Infection of Bovine Cell Lines and Colostrum-Deprived Calves Open
Senecavirus A (SVA) is a causative agent for vesicular disease in swine, which is clinically indistinguishable from other vesicular diseases of swine including foot-and-mouth disease (FMD). Recently, it was reported that buffalo in Guangdo…
View article: Characterization of Senecavirus A Isolates Collected From the Environment of U.S. Sow Slaughter Plants
Characterization of Senecavirus A Isolates Collected From the Environment of U.S. Sow Slaughter Plants Open
Vesicular disease caused by Senecavirus A (SVA) is clinically indistinguishable from foot-and-mouth disease (FMD) and other vesicular diseases of swine. When a vesicle is observed in FMD-free countries, a costly and time-consuming foreign …
View article: Covalent Immune Proximity-Induction Strategy Using SuFEx-Engineered Bifunctional Viral Peptides
Covalent Immune Proximity-Induction Strategy Using SuFEx-Engineered Bifunctional Viral Peptides Open
Harnessing the immune system is a powerful tool in chemical biology and is the focus of cancer immunotherapy. Often, this is accomplished through monoclonal antibodies which recognize and recruit immune effector cells to an over-expressed …
View article: Bacterial translation machinery for deliberate mistranslation of the genetic code
Bacterial translation machinery for deliberate mistranslation of the genetic code Open
Significance Aminoacyl-transfer RNA (tRNA) synthetases (aaRSs) are essential enzymes that mediate accurate expression of the genetic code. More than 96% of analyzed species possess duplicated aaRSs genes, which are presumed to encode canon…
View article: Refinement of an established large-animal model to understand the tick-pathogen-host interface
Refinement of an established large-animal model to understand the tick-pathogen-host interface Open
Ticks are globally distributed vectors of important pathogens of human and animal health. Since the discovery of tick resistance in 1918, the field has sought continuously for the development of effective biologic control of ticks and tick…
View article: A facile immunopeptidomics workflow for capturing the HLA-I ligandome with PEAKS XPro
A facile immunopeptidomics workflow for capturing the HLA-I ligandome with PEAKS XPro Open
Identifying antigens displayed specifically on tumour cell surfaces by human leukocyte antigen (HLA) proteins is important for the development of immunotherapies and cancer vaccines. The difficulty in capturing an HLA ligandome stems from …