Kyle J. Card
YOU?
Author Swipe
View article: Evolution under vancomycin selection drives divergent collateral sensitivity patterns in <i>Staphylococcus aureus</i>
Evolution under vancomycin selection drives divergent collateral sensitivity patterns in <i>Staphylococcus aureus</i> Open
Staphylococcus aureus bacteremia is typically treated empirically with vancomycin, with therapy later tailored based on susceptibility results. However, these tests occur before vancomycin exposure and do not account for adaptation during …
View article: Evolution under vancomycin selection drives divergent collateral sensitivity patterns in <i>Staphylococcus aureus</i>
Evolution under vancomycin selection drives divergent collateral sensitivity patterns in <i>Staphylococcus aureus</i> Open
Staphylococcus aureus bacteremia is typically treated empirically with vancomycin, with therapy later tailored based on susceptibility results. However, these tests occur before vancomycin exposure and do not account for adaptation during …
View article: A low-cost, open-source evolutionary bioreactor and its educational use
A low-cost, open-source evolutionary bioreactor and its educational use Open
A morbidostat is a bioreactor that uses antibiotics to control the growth of bacteria, making it well-suited for studying the evolution of antibiotic resistance. However, morbidostats are often too expensive to be used in educational setti…
View article: Idiosyncratic Fitness Costs of Ampicillin-Resistant Mutants Derived from a Long-Term Experiment with Escherichia coli
Idiosyncratic Fitness Costs of Ampicillin-Resistant Mutants Derived from a Long-Term Experiment with Escherichia coli Open
Antibiotic resistance is a growing concern that has prompted a renewed focus on drug discovery, stewardship, and evolutionary studies of the patterns and processes that underlie this phenomenon. A resistant strain’s competitive fitness rel…
View article: Idiosyncratic fitness costs of ampicillin-resistant mutants derived from a long-term experiment with <i>Escherichia coli</i>
Idiosyncratic fitness costs of ampicillin-resistant mutants derived from a long-term experiment with <i>Escherichia coli</i> Open
Antibiotic resistance is a growing concern that has prompted a renewed focus on drug discovery, stewardship, and evolutionary studies of the patterns and processes that underlie this phenomenon. A resistant strain’s competitive fitness rel…
View article: UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic
UV decontamination of personal protective equipment with idle laboratory biosafety cabinets during the COVID-19 pandemic Open
Personal protective equipment (PPE) is crucially important to the safety of both patients and medical personnel, particularly in the event of an infectious pandemic. As the incidence of Coronavirus Disease 2019 (COVID-19) increases exponen…
View article: Idiosyncratic variation in the fitness costs of tetracycline‐resistance mutations in <i>Escherichia coli</i>
Idiosyncratic variation in the fitness costs of tetracycline‐resistance mutations in <i>Escherichia coli</i> Open
A bacterium's fitness relative to its competitors, both in the presence and absence of antibiotics, plays a key role in its ecological success and clinical impact. In this study, we examine whether tetracycline-resistant mutants are less f…
View article: Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with <i>Escherichia coli</i>
Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with <i>Escherichia coli</i> Open
Significance A fundamental question in evolution is the repeatability of adaptation. Will independently evolving populations respond similarly when facing the same environmental challenge? This question also has important public-health imp…
View article: Idiosyncratic variation in the fitness costs of tetracycline-resistance mutations in<i>Escherichia coli</i>
Idiosyncratic variation in the fitness costs of tetracycline-resistance mutations in<i>Escherichia coli</i> Open
A bacterium’s fitness relative to its competitors, both in the presence and absence of antibiotics, plays a key role in its ecological success and clinical impact. In this study, we examine whether tetracycline-resistant mutants are less f…
View article: Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with<i>Escherichia coli</i>
Genomic evolution of antibiotic resistance is contingent on genetic background following a long-term experiment with<i>Escherichia coli</i> Open
Antibiotic resistance is a growing health concern. Efforts to control resistance would benefit from an improved ability to forecast when and how it will evolve. Epistatic interactions between mutations can promote divergent evolutionary tr…
View article: UV Sterilization of Personal Protective Equipment with Idle Laboratory Biosafety Cabinets During the COVID-19 Pandemic
UV Sterilization of Personal Protective Equipment with Idle Laboratory Biosafety Cabinets During the COVID-19 Pandemic Open
DISCLAIMER This article does not represent the official recommendation of the Cleveland Clinic or Case Western Reserve University School of Medicine, nor has it yet been peer reviewed. We are releasing it early, pre-peer review, to allow f…
View article: Historical contingency in the evolution of antibiotic resistance after decades of relaxed selection
Historical contingency in the evolution of antibiotic resistance after decades of relaxed selection Open
Populations often encounter changed environments that remove selection for the maintenance of particular phenotypic traits. The resulting genetic decay of those traits under relaxed selection reduces an organism's fitness in its prior envi…
View article: A low-cost, open-source evolutionary bioreactor and its educational use
A low-cost, open-source evolutionary bioreactor and its educational use Open
The morbidostat automatically adjusts antibiotic concentration as a bacterial population evolves resistance. Although this device has advanced our understanding of the evolutionary and ecological processes that drive antibiotic resistance,…
View article: Historical contingency in the evolution of antibiotic resistance after decades of relaxed selection
Historical contingency in the evolution of antibiotic resistance after decades of relaxed selection Open
Populations often encounter changed environments that remove selection for the maintenance of particular phenotypic traits. The resulting genetic decay of those traits under relaxed selection reduces an organism’s fitness in its prior envi…