Kyle Shifflett
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View article: CD81 fusion alters SARS-CoV-2 Spike trafficking.
CD81 fusion alters SARS-CoV-2 Spike trafficking. Open
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic caused the biggest public health crises in recent history. Many expect future coronavirus introductions into the human population. Hence, it is essential to understa…
View article: CD81 fusion alters SARS-CoV-2 Spike trafficking
CD81 fusion alters SARS-CoV-2 Spike trafficking Open
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic caused the biggest public health crises in recent history. Many expect future coronavirus introductions into the human population. Hence, it is essential to understa…
View article: Large‐scale heparin‐based bind‐and‐elute chromatography identifies two biologically distinct populations of extracellular vesicles
Large‐scale heparin‐based bind‐and‐elute chromatography identifies two biologically distinct populations of extracellular vesicles Open
Purifying extracellular vesicles (EVs) has been challenging because EVs are heterogeneous in cargo yet share similar sizes and densities. Most surface marker‐based affinity separation methods are limited to research or diagnostic scales. W…
View article: VSV-Based Vaccines Reduce Virus Shedding and Viral Load in Hamsters Infected with SARS-CoV-2 Variants of Concern
VSV-Based Vaccines Reduce Virus Shedding and Viral Load in Hamsters Infected with SARS-CoV-2 Variants of Concern Open
The continued progression of the COVID-19 pandemic can partly be attributed to the ability of SARS-CoV-2 to mutate and introduce new viral variants. Some of these variants with the potential to spread quickly and conquer the globe are term…
View article: Serum from COVID-19 patients early in the pandemic shows limited evidence of cross-neutralization against variants of concern
Serum from COVID-19 patients early in the pandemic shows limited evidence of cross-neutralization against variants of concern Open
View article: Rapid Protection from COVID-19 in Nonhuman Primates Vaccinated Intramuscularly but Not Intranasally with a Single Dose of a Vesicular Stomatitis Virus-Based Vaccine
Rapid Protection from COVID-19 in Nonhuman Primates Vaccinated Intramuscularly but Not Intranasally with a Single Dose of a Vesicular Stomatitis Virus-Based Vaccine Open
The vesicular stomatitis virus (VSV) vaccine platform rose to fame in 2019, when a VSV-based Ebola virus (EBOV) vaccine was approved by the European Medicines Agency and the U.S. Food and Drug Administration for human use against the deadl…
View article: Optimization of Single-Dose VSV-Based COVID-19 Vaccination in Hamsters
Optimization of Single-Dose VSV-Based COVID-19 Vaccination in Hamsters Open
The ongoing COVID-19 pandemic has resulted in global effects on human health, economic stability, and social norms. The emergence of viral variants raises concerns about the efficacy of existing vaccines and highlights the continued need f…
View article: Serum from COVID-19 patients early in the pandemic shows limited evidence of cross-neutralization against variants of concern
Serum from COVID-19 patients early in the pandemic shows limited evidence of cross-neutralization against variants of concern Open
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) results in a variety of clinical symptoms ranging from no or mild to severe disease. Currently, there are multiple postulated mechanisms that may push a moderate to severe diseas…
View article: Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model
Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model Open
View article: The Ebola virus soluble glycoprotein contributes to viral pathogenesis by activating the MAP kinase signaling pathway
The Ebola virus soluble glycoprotein contributes to viral pathogenesis by activating the MAP kinase signaling pathway Open
Ebola virus (EBOV) expresses three different glycoproteins (GPs) from its GP gene. The primary product, soluble GP (sGP), is secreted in abundance during infection. EBOV sGP has been discussed as a potential pathogenicity factor, however, …
View article: Optimization of single dose VSV-based COVID-19 vaccination in hamsters
Optimization of single dose VSV-based COVID-19 vaccination in hamsters Open
The ongoing COVID-19 pandemic has resulted in global effects on human health, economic stability, and social norms. The emergence of viral variants raises concerns about the efficacy of existing vaccines and highlights the continued need t…
View article: Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model
Pathogenic and transcriptomic differences of emerging SARS-CoV-2 variants in the Syrian golden hamster model Open
Following the discovery of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its rapid spread throughout the world, new viral variants of concern (VOC) have emerged. There is a critical need to understand the impact of the e…
View article: A human factor H-binding protein of<i>Bartonella bacilliformis</i>and potential role in serum resistance
A human factor H-binding protein of<i>Bartonella bacilliformis</i>and potential role in serum resistance Open
Bartonella bacilliformis is a Gram-negative bacterium and etiologic agent of Carrión’s disease; a potentially life-threatening illness endemic to South America. B. bacilliformis is a facultative parasite that infects human erythrocytes (he…
View article: Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a recombinant vaccine
Rapid protection from COVID-19 in nonhuman primates vaccinated intramuscularly but not intranasally with a single dose of a recombinant vaccine Open
The ongoing pandemic of Coronavirus disease 2019 (COVID-19) continues to exert a significant burden on health care systems worldwide. With limited treatments available, vaccination remains an effective strategy to counter transmission of s…
View article: The Ebola virus soluble glycoprotein contributes to viral pathogenesis by activating the MAP kinase signaling pathway
The Ebola virus soluble glycoprotein contributes to viral pathogenesis by activating the MAP kinase signaling pathway Open
Data set use to generate the figures in the manuscript "The Ebola virus soluble glycoprotein contributes to viral pathogenesis by activating the MAP kinase signaling pathway" by Furuyama and colleagues, Plos Pathogens 2021.
View article: Marburg virus pathogenesis – differences and similarities in humans and animal models
Marburg virus pathogenesis – differences and similarities in humans and animal models Open
Marburg virus (MARV) is a highly pathogenic virus associated with severe disease and mortality rates as high as 90%. Outbreaks of MARV are sporadic, deadly, and often characterized by a lack of resources and facilities to diagnose and trea…
View article: A CsrA-Binding, <i>trans</i> -Acting sRNA of <i>Coxiella burnetii</i> Is Necessary for Optimal Intracellular Growth and Vacuole Formation during Early Infection of Host Cells
A CsrA-Binding, <i>trans</i> -Acting sRNA of <i>Coxiella burnetii</i> Is Necessary for Optimal Intracellular Growth and Vacuole Formation during Early Infection of Host Cells Open
Regulation of metabolism and virulence in C. burnetii is not well understood. Here, we show that C. burnetii small RNA 12 (CbsR12) is highly transcribed in the metabolically active large-cell variant compared to the nonreplicative small-ce…
View article: <i>Coxiella burnetii</i>small RNA 12 binds CsrA regulatory protein and transcripts for the CvpD type IV effector, regulates pyrimidine and methionine metabolism, and is necessary for optimal intracellular growth and vacuole formation during infection
<i>Coxiella burnetii</i>small RNA 12 binds CsrA regulatory protein and transcripts for the CvpD type IV effector, regulates pyrimidine and methionine metabolism, and is necessary for optimal intracellular growth and vacuole formation during infection Open
Coxiella burnetii is an obligate intracellular gammaproteobacterium and zoonotic agent of Q fever. We previously identified 15 small non-coding RNAs (sRNAs) of C. burnetii . One of them, named CbsR12 ( C oxiella b urnetii s mall R NA 12 ) …
View article: A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle Open
View article: Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses
Lifecycle modelling systems support inosine monophosphate dehydrogenase (IMPDH) as a pro-viral factor and antiviral target for New World arenaviruses Open
View article: A Single Dose of Modified Vaccinia Ankara expressing Ebola Virus Like Particles Protects Nonhuman Primates from Lethal Ebola Virus Challenge
A Single Dose of Modified Vaccinia Ankara expressing Ebola Virus Like Particles Protects Nonhuman Primates from Lethal Ebola Virus Challenge Open
Ebola virus (EBOV), isolate Makona, was the causative agent of the West African epidemic devastating predominantly Guinea, Liberia and Sierra Leone from 2013–2016. While several experimental vaccine and treatment approaches have been accel…
View article: Additional file 5: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
Additional file 5: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle Open
Results of secondary screen, excel file format. (XLSX 50 kb)
View article: Additional file 6: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
Additional file 6: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle Open
Results of secondary assay, excel file format. (XLSX 18 kb)
View article: Additional file 2: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
Additional file 2: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle Open
Uncorrected and seed-corrected logP values from RSA analysis, excel file format. (XLSX 1121 kb)
View article: Additional file 3: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle
Additional file 3: of A genome-wide siRNA screen identifies a druggable host pathway essential for the Ebola virus life cycle Open
Genes selected for follow-up, excel file format. (XLSX 14 kb)