Kyohei Higashi
YOU?
Author Swipe
View article: A Novel Chimeric Molecule of Heparanase and Ig-Fc Enables Histochemical and Cytochemical Detection of O-sulfated Heparan Sulfate
A Novel Chimeric Molecule of Heparanase and Ig-Fc Enables Histochemical and Cytochemical Detection of O-sulfated Heparan Sulfate Open
A chimeric protein of heparanase and Ig-Fc was designed as a novel tool to expand the detection of structurally heterogeneous heparan sulfate (HS) and related glycosaminoglycans. The whole mouse heparanase gene was combined with the gene s…
View article: Isolating and Determining the Structures of Colored Products from the Reactions of Cannabinoids with Fast Blue RR
Isolating and Determining the Structures of Colored Products from the Reactions of Cannabinoids with Fast Blue RR Open
Although cannabis is used in a wide range of fields, including medicine and pharmacology, its use is prohibited in Japan because it contains Δ9-tetrahydrocannabinol (Δ9-THC), a compound that exhibits narcotic effects. While cannabis is pri…
View article: Polyamines stimulate the protein synthesis of the translation initiation factor eIF5A2, participating in mRNA decoding, distinct from eIF5A1
Polyamines stimulate the protein synthesis of the translation initiation factor eIF5A2, participating in mRNA decoding, distinct from eIF5A1 Open
Polyamines are present in all living organisms, and their homeostasis is closely associated with human health and disease. Furthermore, they are small aliphatic cations that exhibit multifunctional activities through interactions with acid…
View article: Development of a method for simultaneous analysis of glycosaminoglycan disaccharides and evaluating the quality of chondroitin sulfate and hyaluronic acid in food raw materials
Development of a method for simultaneous analysis of glycosaminoglycan disaccharides and evaluating the quality of chondroitin sulfate and hyaluronic acid in food raw materials Open
We developed a comprehensive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the simultaneous analysis of 19 glycosaminoglycan disaccharide components. Although structural similarity among components can hinder MS iden…
View article: The effects of WWP1 overexpression on the golgi apparatus stress response and proteoglycan production in adipocytes
The effects of WWP1 overexpression on the golgi apparatus stress response and proteoglycan production in adipocytes Open
White adipocytes are a major component of white adipose tissue (WAT) and help to maintain systemic metabolic homeostasis by storing energy and secreting adipokines. In mice deficient in the protein WWP1 (WW domain-containing E3 ubiquitin p…
View article: Correction: A novel cytoskeletal action of xylosides
Correction: A novel cytoskeletal action of xylosides Open
[This corrects the article DOI: 10.1371/journal.pone.0269972.].
View article: The Uptake of Heparanase into Mast Cells Is Regulated by Its Enzymatic Activity to Degrade Heparan Sulfate
The Uptake of Heparanase into Mast Cells Is Regulated by Its Enzymatic Activity to Degrade Heparan Sulfate Open
Mast cells take up extracellular latent heparanase and store it in secretory granules. The present study examined whether the enzymatic activity of heparanase regulates its uptake efficiency. Recombinant mouse heparanase mimicking both the…
View article: Development of an Analytical Method for Unsaturated Disaccharides from Heparan Sulfate Using Dansylhydrazine Derivatization and High-Performance Liquid Chromatography with Fluorescence Detection
Development of an Analytical Method for Unsaturated Disaccharides from Heparan Sulfate Using Dansylhydrazine Derivatization and High-Performance Liquid Chromatography with Fluorescence Detection Open
Glycosaminoglycans (GAGs), such as heparan sulfate (HS), play essential roles in living organisms. Understanding the functionality of HS and its involvement in disease progression necessitates the sensitive and quantitative detection of HS…
View article: WWP1 localizes in the Golgi apparatus and contributes to maintaining glycosaminoglycan synthesis in adipocytes
WWP1 localizes in the Golgi apparatus and contributes to maintaining glycosaminoglycan synthesis in adipocytes Open
White adipocytes are a major component of white adipose tissue (WAT) and help to maintain systemic metabolic homeostasis because they store energy and secrete adipokines. In mice deficient in the protein WWP1 (WW domain-containing E3 ubiqu…
View article: Chemical genetic interaction linking eIF5A hypusination and mitochondrial integrity
Chemical genetic interaction linking eIF5A hypusination and mitochondrial integrity Open
The eukaryotic translation factor eIF5A plays an important role in translation elongation, especially across stretches of prolines and charged amino acids, and in translation termination. eIF5A undergoes hypusination, a post-translational …
View article: Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection
Analysis of Acetylated Hyaluronic Acid in Moisturizing Lotion and Milk Lotion by HPLC with Fluorescence Detection Open
We developed a simple and sensitive analytical HPLC method for the determination of acetylated hyaluronic acid (AcHA) in moisturizing and milk lotions. AcHA with different molecular weights was separated as a single peak using a C4 column …
View article: Fucosylated heparan sulfate from the midgut gland of Patinopecten yessoensis
Fucosylated heparan sulfate from the midgut gland of Patinopecten yessoensis Open
The structural and functional relationships of glycosaminoglycans (GAGs) derived from marine organisms have been investigated, suggesting that marine invertebrates, particularly Bivalvia, are abundant sources of highly sulfated or branched…
View article: A search for acrolein scavengers among food components
A search for acrolein scavengers among food components Open
Brain stroke is a major cause of being bedridden for elderly people, and preventing stroke is important for maintaining quality of life (QOL). Acrolein is a highly reactive aldehyde and causes tissue damage during stroke. Decreasing acrole…
View article: A Novel Cytoskeletal Action of Xylosides
A Novel Cytoskeletal Action of Xylosides Open
Proteoglycan glycosaminoglycan (GAG) chains are attached to a serine residue in the protein through a linkage series of sugars, the first of which is xylose. Xylosides are chemicals which compete with the xylose at the enzyme xylosyl trans…
View article: Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats
Characterization of chondroitin sulfate in stem cells derived from umbilical cord blood in rats Open
Chondroitin sulfate (CS) and its isomeric variant, dermatan sulfate (DS), are complex glycosaminoglycans (GAGs) which are ubiquitous components of the extracellular matrix in various tissues including the brain. CS and/or DS are known to b…
View article: Structure and Immunomodulatory Activity of Glycogen Derived from Honeybee Larvae (<i>Apis mellifera</i>)
Structure and Immunomodulatory Activity of Glycogen Derived from Honeybee Larvae (<i>Apis mellifera</i>) Open
Honeybee larvae have been recognized as nutrient-rich food in many countries. Although glycogen, a storage form of glucose in animals, is synthesized in honeybee larvae, there is no information on the structure of glycan and its biological…
View article: Chemicals in aerosols generated from heated tobacco products and their biological effects
Chemicals in aerosols generated from heated tobacco products and their biological effects Open
喫煙者の健康志向の高まりや2020年4月から完全施行された改正健康増進法を受けて，紙巻きたばこの喫煙から加熱式たばこ製品の使用へと，喫煙者の行動変容が進んでいる。それに伴い，加熱式たばこ製品の開発競争も激しさを増し，加熱式たばこの加熱装置や専用たばこスティックの種類は，年々増加の一途をたどっている。加熱式たばこでは，主流煙中の化学物質量が低減されているが，能動喫煙者や受動喫煙者の健康リスクに関する科学的エビデンスは圧倒的に少ない。また，たばこ主流煙の吸煙プロトコールとして，…
View article: Trehalose induces SQSTM1/p62 expression and enhances lysosomal activity and antioxidative capacity in adipocytes
Trehalose induces SQSTM1/p62 expression and enhances lysosomal activity and antioxidative capacity in adipocytes Open
Adipocytes, which comprise the majority of white adipose tissue (WAT), are involved in obesity‐related pathology via various mechanisms, including disturbed lysosomal enzymatic activity and accumulation of oxidative stress. Sequestosome 1 …
View article: Expression of Glycosaminoglycan-Related Genes and the Role of Polyamines in the Glycosaminoglycan Biosynthetic Pathways
Expression of Glycosaminoglycan-Related Genes and the Role of Polyamines in the Glycosaminoglycan Biosynthetic Pathways Open
Glycosaminoglycans (GAGs), a group of structurally related acidic polysaccharides, are primarily present as glycan moieties of proteoglycans (PGs). The physiological functions of PGs depend on the interaction between the glycan moieties an…
View article: Expression of Glycosaminoglycan-Related Genes and the Role of Polyamines in the Glycosaminoglycan Biosynthetic Pathways
Expression of Glycosaminoglycan-Related Genes and the Role of Polyamines in the Glycosaminoglycan Biosynthetic Pathways Open
酸性多糖類であるグリコサミノグリカン （GAG）は、コアタンパク質と共有結合したプロテオグリカン （PG）として生体内に存在する。 PGの生物活性は、糖鎖とGAG結合タンパク質 （成長因子、ケモカイン、接着分子など）との相互作用に基づいている。そのため、GAG鎖の構造、すなわち硫酸化パターン、エピメル化および分子量はGAG結合タンパク質との相互作用に重要である。しかしながら、何故GAG鎖の構造が分化や老化に伴って変化するのか、その詳細なメカニズムはほとんど明らかとなっていな…
View article: Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction
Detection of Selenocyanate in Biological Samples by HPLC with Fluorescence Detection Using König Reaction Open
We developed a simple and sensitive HPLC method for the determination of selenocyanate (SeCN-). The König reaction, which is generally used for the determination of cyanide and thiocyanate, was applied for the post-column detection, and us…
View article: Preparation of <sup>99m</sup>Tc-Labeled Mannan-<i>S</i>-Cysteine and Effect of Molecular Size of Mannan on Its Biodistribution
Preparation of <sup>99m</sup>Tc-Labeled Mannan-<i>S</i>-Cysteine and Effect of Molecular Size of Mannan on Its Biodistribution Open
Macrophage mannose receptor (MMR/CD206) is a promising target for the detection and identification of sentinel lymph node (SLN). MMR-targeting probes have been developed using mannosylated dextran, however, impairment of efficient targetin…
View article: Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII
Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII Open
海洋生物、特に二枚貝は抗凝固薬ヘパリンの代替資源として知られている。コンドロイチン硫酸（CS）もヘパリンと同様に二枚貝から抽出されるが、CSの詳細な構造解析は行われなかった。近年、私達は11種の食用貝から抽出したCSをコンドロイチナーゼABC又はACIIで処理し、得られた不飽和二糖をHPLCで分析した。その結果、いくつかの貝類で、未同定ピークがコンドロイチナーゼABCではなくコンドロイチナーゼACII処理により検出された。バカガイのCSでは、グルクロン酸のC-3位にケラタン…
View article: Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII
Identification of Chondroitin Sulfates Having Unique Structures from Shellfish Using Chondroitinase ACII Open
Marine organisms, especially bivalve molluscs have been known as an alternative source of heparin, an anticoagulant. Although chondroitin sulfate (CS) can be also extracted together with heparin, the detailed glycan structures of CS have n…
View article: The Structural and Compositional Changes of Chondroitin Sulfate Chains in the Aged Mouse Hippocampus
The Structural and Compositional Changes of Chondroitin Sulfate Chains in the Aged Mouse Hippocampus Open
The hippocampus plays important roles in learning and memory. In the hippocampus, the extracellular matrix (ECM) is known to function in synaptic plasticity. Chondroitin sulfate (CS) chain, one of the major ECM components in the brain, is …
View article: Protective Effects of Brain Infarction by<i>N</i>-Acetylcysteine Derivatives
Protective Effects of Brain Infarction by<i>N</i>-Acetylcysteine Derivatives Open
Background and Purpose— We recently found that acrolein (CH 2 =CH-CHO) is more strongly involved in brain infarction compared with reactive oxygen species. In this study, we looked for acrolein scavengers with less side effects. Methods— P…
View article: Reduction of Spermidine Content Resulting from Inactivation of Two Arginine Decarboxylases Increases Biofilm Formation in Synechocystis sp. Strain PCC 6803
Reduction of Spermidine Content Resulting from Inactivation of Two Arginine Decarboxylases Increases Biofilm Formation in Synechocystis sp. Strain PCC 6803 Open
The phototropic bacterium Synechocystis sp. strain PCC 6803 is able to adapt its morphology in order to survive in a wide range of harsh environments. Under conditions of high salinity, planktonic cells formed cell aggregates in culture. F…