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View article: A Change in Liver Metabolism but Not in Brown Adipose Tissue Thermogenesis Is an Early Event in Ovariectomy-Induced Obesity in Rats
A Change in Liver Metabolism but Not in Brown Adipose Tissue Thermogenesis Is an Early Event in Ovariectomy-Induced Obesity in Rats Open
Menopause is associated with increased visceral adiposity and disrupted glucose homeostasis, but the underlying molecular mechanisms related to these metabolic changes are still elusive. Brown adipose tissue (BAT) plays a key role in energ…
View article: How Enzymes Handle the Energy Derived from the Cleavage of High-energy Phosphate Compounds
How Enzymes Handle the Energy Derived from the Cleavage of High-energy Phosphate Compounds Open
I was accepted at the Federal University of Rio de Janeiro Medical School when I was seventeen years old. At that time, I was absolutely sure I was going to be a surgeon. One year later, I received an offer for a fellowship to work in a re…
View article: Brown adipose tissue mitochondria: modulation by GDP and fatty acids depends on the respiratory substrates
Brown adipose tissue mitochondria: modulation by GDP and fatty acids depends on the respiratory substrates Open
The UCP1 [first UCP (uncoupling protein)] that is found in the mitochondria of brown adipocytes [BAT (brown adipose tissue)] regulates the heat production, a process linked to non-shivering thermogenesis. The activity of UCP1 is modulated …
View article: Fusion of the Endoplasmic Reticulum and Mitochondrial Outer Membrane in Rats Brown Adipose Tissue: Activation of Thermogenesis by Ca2+
Fusion of the Endoplasmic Reticulum and Mitochondrial Outer Membrane in Rats Brown Adipose Tissue: Activation of Thermogenesis by Ca2+ Open
Brown adipose tissue (BAT) mitochondria thermogenesis is regulated by uncoupling protein 1 (UCP 1), GDP and fatty acids. In this report, we observed fusion of the endoplasmic reticulum (ER) membrane with the mitochondrial outer membrane of…
View article: Identification of Emotional Components to Improve the Motivation for Science Among School Teachers
Identification of Emotional Components to Improve the Motivation for Science Among School Teachers Open
Science education has been searching for new strategies to involve students in scientific investigation processes. These strategies should stimulate questioning, predicting results by means of experimentation as well as developing abilitie…
View article: LUDIC APPROACH OF THE SCIENTIFIC METHOD IN BIOCHEMICAL EDUCATION
LUDIC APPROACH OF THE SCIENTIFIC METHOD IN BIOCHEMICAL EDUCATION Open
Education in the current times concerns to the intellectual autonomy, the capacity of decision and the possibilities of the student in making the difference. This work arose due to multi-transdisciplinary perception of an educator’s team f…
View article: CREATING EDUCATIONAL MATERIAL TO HIGH SCHOOL EDUCATION WITH COMIC BOOKS: THE VACCINA´S HISTORY.
CREATING EDUCATIONAL MATERIAL TO HIGH SCHOOL EDUCATION WITH COMIC BOOKS: THE VACCINA´S HISTORY. Open
The Comic book "The Vaccine’s History" is part of a project that creates educational material intended to improve high school education, and is being developed for approximately ten years at the Bioenergetics Laboratory under Prof. Leopold…
View article: Conformational Fluctuations of the Ca2+-ATPase in the Native Membrane Environment
Conformational Fluctuations of the Ca2+-ATPase in the Native Membrane Environment Open
Digestion with proteinase K or trypsin yields complementary information on conformational transitions of the Ca(2+)-ATPase (SERCA) in the native membrane environment. Distinct digestion patterns are obtained with proteinase K, revealing in…
View article: The impact of science in Brazil
The impact of science in Brazil Open
IUBMB Life is the flagship journal of the International Union of Biochemistry and Molecular Biology and is devoted to the rapid publication of the most novel and significant short articles, reviews and papers in the broadly defined fields …
View article: Identification of a Ca2+-ATPase in Brown Adipose Tissue Mitochondria
Identification of a Ca2+-ATPase in Brown Adipose Tissue Mitochondria Open
In brown adipose tissue (BAT) adrenaline promotes a rise of the cytosolic Ca(2+) concentration from 0.05 up to 0.70 mum. It is not known how the rise of Ca(2+) concentration activates BAT thermogenesis. In this report we compared the effec…
View article: The Thermogenic Activity of Rat Brown Adipose Tissue and Rabbit White Muscle Ca2 + ‐ATPase
The Thermogenic Activity of Rat Brown Adipose Tissue and Rabbit White Muscle Ca2 + ‐ATPase Open
The Ca2 + ‐ATPase (SERCA) found in vesicles derived from the sarco/endoplasmic reticulum vesicles of rats brown adipose tissue and rabbit white muscle were identified by gel electrophoresis, Western blot, electron microscopy and immunolabe…
View article: Thyroid hormones differentially regulate the distribution of rabbit skeletal muscle Ca<sup>2 + </sup>-ATPase (SERCA) isoforms in light and heavy sarcoplasmic reticulum
Thyroid hormones differentially regulate the distribution of rabbit skeletal muscle Ca<sup>2 + </sup>-ATPase (SERCA) isoforms in light and heavy sarcoplasmic reticulum Open
The sarcoplasmic reticulum (SR) is composed of two fractions, the heavy fraction that contains proteins involved in Ca2+ release, and the light fraction enriched in Ca(2+)-ATPase (SERCA), an enzyme responsible for Ca2+ transport from the c…
View article: Heat of PPi Hydrolysis Varies Depending on the Enzyme Used
Heat of PPi Hydrolysis Varies Depending on the Enzyme Used Open
With yeast-soluble inorganic pyrophosphatase, the heat released during PP(i) hydrolysis was -6.3 kcal/mol regardless of the KCl concentration in the medium. With the membrane-bound pyrophosphatase of corn vacuoles, the heat released varies…
View article: Mitochondrial Bound Hexokinase Activity as a Preventive Antioxidant Defense
Mitochondrial Bound Hexokinase Activity as a Preventive Antioxidant Defense Open
Brain hexokinase is associated with the outer membrane of mitochondria, and its activity has been implicated in the regulation of ATP synthesis and apoptosis. Reactive oxygen species (ROS) are by-products of the electron transport chain in…
View article: Hyperthyroidism increases the uncoupled ATPase activity and heat production by the sarcoplasmic reticulum Ca2+-ATPase
Hyperthyroidism increases the uncoupled ATPase activity and heat production by the sarcoplasmic reticulum Ca2+-ATPase Open
The sarcoplasmic reticulum Ca2+-ATPase is able to modulate the distribution of energy released during ATP hydrolysis, so that a portion of energy is used for Ca2+ transport (coupled ATPase activity) and a portion is converted into heat (un…
View article: Proton Transport in Maize Tonoplasts Supported by Fructose-1,6-Bisphosphate Cleavage. Pyrophosphate-Dependent Phosphofructokinase as a Pyrophosphate-Regenerating System
Proton Transport in Maize Tonoplasts Supported by Fructose-1,6-Bisphosphate Cleavage. Pyrophosphate-Dependent Phosphofructokinase as a Pyrophosphate-Regenerating System Open
The energy derived from pyrophosphate (PPi) hydrolysis is used to pump protons across the tonoplast membrane, thus forming a proton gradient. In a plant's cytosol, the concentration of PPi varies between 10 and 800 μm, and the PPi concentr…
View article: Brown Adipose Tissue Ca2+-ATPase
Brown Adipose Tissue Ca2+-ATPase Open
In this report a sarco/endoplasmic reticulum Ca2+-ATPase (SERCA) was identified in rats brown adipose tissue. Electrophoretic analysis of brown fat microssomal protein yields a 110-kDa band that is reactive to SERCA 1 antibody but is not r…
View article: The transfer of Molecular Biology and Genetic engineerings knowlodge to high school: problems and solutions
The transfer of Molecular Biology and Genetic engineerings knowlodge to high school: problems and solutions Open
of the panel presented at the SBBq annual meeting (see attachament).
View article: The Brazilian graduate evaluation
The Brazilian graduate evaluation Open
of the panel presented at the SBBq annual meeting (see attachament).
View article: Uncoupled ATP Hydrolysis and Thermogenic Activity of the Sarcoplasmic Reticulum Ca2+-ATPase
Uncoupled ATP Hydrolysis and Thermogenic Activity of the Sarcoplasmic Reticulum Ca2+-ATPase Open
The sarcoplasmic reticulum Ca(2+)-ATPase transports Ca(2+) using the energy derived from ATP hydrolysis. During catalysis, part of the energy is used to translocate Ca(2+) across the membrane, and part is dissipated as heat. At 35 degrees …
View article: Thermogenesis and energy expenditure: control of heat production by the Ca 2+ -ATPase of fast and slow muscle
Thermogenesis and energy expenditure: control of heat production by the Ca 2+ -ATPase of fast and slow muscle Open
This report measured the amount of heat released during Ca(2+) transport and ATP hydrolysis by vesicles derived from the sarcoplasmic reticulum of rabbit slow (SM) and fast (FM) muscle. During ATP hydrolysis, part of the chemical energy re…
View article: Correlation between Uncoupled ATP Hydrolysis and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase
Correlation between Uncoupled ATP Hydrolysis and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase Open
The sarcoplasmic reticulum Ca(2+)-ATPase transports Ca(2+) using the chemical energy derived from ATP hydrolysis. Part of the chemical energy is used to translocate Ca(2+) through the membrane (work) and part is dissipated as heat. The amo…
View article: Uncoupled ATPase Activity and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase
Uncoupled ATPase Activity and Heat Production by the Sarcoplasmic Reticulum Ca2+-ATPase Open
Sarcoplasmic reticulum vesicles of rabbit skeletal muscle accumulate Ca2+ at the expense of ATP hydrolysis. The heat released during the hydrolysis of each ATP molecule varies depending on whether or not a Ca2+ gradient is formed across th…
View article: Energy interconversion by the sarcoplasmic reticulum Ca2+-ATPase: ATP hydrolysis, Ca2+ transport, ATP synthesis and heat production
Energy interconversion by the sarcoplasmic reticulum Ca2+-ATPase: ATP hydrolysis, Ca2+ transport, ATP synthesis and heat production Open
The sarcoplasmic reticulum of skeletal muscle retains a membrane bound Ca2+-ATPase which is able to interconvert different forms of energy. A part of the chemical energy released during ATP hydrolysis is converted into heat and in the bibl…
View article: Correlation between MSc./ Ph.D and scientific production in Brazil
Correlation between MSc./ Ph.D and scientific production in Brazil Open
of the panel presented at the SBBq annual meeting (see attachament).
View article: Contradictory scientific concepts among didatic books, students, teachers and scientists
Contradictory scientific concepts among didatic books, students, teachers and scientists Open
of the panel presented at the SBBq annual meeting (see attachament).
View article: Ca2+ Release and Heat Production by the Endoplasmic Reticulum Ca2+-ATPase of Blood Platelets
Ca2+ Release and Heat Production by the Endoplasmic Reticulum Ca2+-ATPase of Blood Platelets Open
Different sarco/endoplasmic reticulum Ca(2+)-ATPases isoforms are found in blood platelets and in skeletal muscle. The amount of heat produced during ATP hydrolysis by vesicles derived from the endoplasmic reticulum of blood platelets was …
View article: What the teachers believe: difficulties and suggestions
What the teachers believe: difficulties and suggestions Open
of the panel presented at the SBBq annual meeting (see Attachment).