Lukas Heger
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View article: GM-CSF and specific type 2 cytokines induce CD103+ and CD301b+ cell states in cDC1s and cDC2s
GM-CSF and specific type 2 cytokines induce CD103+ and CD301b+ cell states in cDC1s and cDC2s Open
The heterogeneity of conventional dendritic cells type 1 (cDC1s) and type 2 (cDC2s) is well established, yet the identity and origin of CD301b+ cDC2s remain debated. Here, we show that CD301b+ cDC2s and CD103+ cDC1s develop from pre-commit…
View article: Molecular Characterization of the GALC Mutation Thr112Ala Causing Krabbe Disease
Molecular Characterization of the GALC Mutation Thr112Ala Causing Krabbe Disease Open
Krabbe disease is a rare and severe lysosomal disorder affecting the white matter of the central and peripheral nervous system. It is characterized by neurodegeneration, with the most common form being infantile Krabbe disease, typically d…
View article: Extracorporeal Photopheresis: Secreted Factors That Promote Immunomodulation
Extracorporeal Photopheresis: Secreted Factors That Promote Immunomodulation Open
Background. Extracorporeal photopheresis (ECP) is a therapy indicated for various T cell–mediated conditions, including cutaneous T-cell lymphoma (CTCL), graft-versus-host disease (GVHD), and solid organ transplant rejection. ECP comprises…
View article: Extracorporeal photopheresis reduces the T cell stimulatory capacity of human primary blood conventional dendritic cells type 1
Extracorporeal photopheresis reduces the T cell stimulatory capacity of human primary blood conventional dendritic cells type 1 Open
Introduction Extracorporeal photopheresis (ECP) is an immunomodulatory treatment option for different T cell-mediated diseases such as cutaneous T cell lymphoma (CTCL) and chronic graft-versus-host disease (GvHD). While in CTCL the polariz…
View article: Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC
Guidelines for preparation and flow cytometry analysis of human nonlymphoid tissue DC Open
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional …
View article: Anti-CD19 CAR-T cells are effective in severe idiopathic Lambert-Eaton myasthenic syndrome
Anti-CD19 CAR-T cells are effective in severe idiopathic Lambert-Eaton myasthenic syndrome Open
Lambert-Eaton myasthenic syndrome (LEMS) is an autoantibody-mediated disease of the neuromuscular junction characterized by muscular weakness. Autoantibodies to presynaptic P/Q-type voltage-gated calcium channels (VGCCs) induce defective n…
View article: Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s
Clec12A, CD301b, and FcγRIIB/III define the heterogeneity of murine DC2s and DC3s Open
Over the last decade, multiple studies have investigated the heterogeneity of murine conventional dendritic cells type 2 (cDC2s). However, their phenotypic similarity with monocytes and macrophages renders their clear identification challe…
View article: Unbiased high-dimensional flow cytometry identified NK and DC immune cell signature in Luminal A-type and triple negative breast cancer
Unbiased high-dimensional flow cytometry identified NK and DC immune cell signature in Luminal A-type and triple negative breast cancer Open
Breast cancer is the most common malignancy in women worldwide and a highly heterogeneous disease. Four different subtypes are described that differ in the expression of hormone receptors as well as the growth factor receptor HER2. Treatme…
View article: XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector functions from their immediate precursors
XCR1 expression distinguishes human conventional dendritic cell type 1 with full effector functions from their immediate precursors Open
Dendritic cells (DCs) are major regulators of innate and adaptive immune responses. DCs can be classified into plasmacytoid DCs and conventional DCs (cDCs) type 1 and 2. Murine and human cDC1 share the mRNA expression of XCR1. Murine studi…
View article: Circumventing pyroptosis via hyperactivation shapes superior immune responses of human type 2 dendritic cells compared to type 3 dendritic cells
Circumventing pyroptosis via hyperactivation shapes superior immune responses of human type 2 dendritic cells compared to type 3 dendritic cells Open
Exploiting inflammasome activation in dendritic cells (DCs) is a promising approach to fight cancer and to augment adjuvant‐induced immune responses. As inflammasome formation is typically accompanied by pyroptosis, hyperactivation—defined…
View article: Guidelines for mouse and human DC functional assays
Guidelines for mouse and human DC functional assays Open
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional …
View article: Guidelines for DC preparation and flow cytometric analysis of human lymphohematopoietic tissues
Guidelines for DC preparation and flow cytometric analysis of human lymphohematopoietic tissues Open
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional …
View article: Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues
Guidelines for DC preparation and flow cytometry analysis of mouse nonlymphoid tissues Open
This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state‐of‐the‐art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy and functional c…
View article: Modulation of urelumab glycosylation separates immune stimulatory activity from organ toxicity
Modulation of urelumab glycosylation separates immune stimulatory activity from organ toxicity Open
Checkpoint control and immunomodulatory antibodies have become important tools for modulating tumor or self-reactive immune responses. A major issue preventing to make full use of the potential of these immunomodulatory antibodies are the …
View article: Monocytes Elicit a Neutrophil-Independent Th1/Th17 Response Upon Immunization With a Mincle-Dependent Glycolipid Adjuvant
Monocytes Elicit a Neutrophil-Independent Th1/Th17 Response Upon Immunization With a Mincle-Dependent Glycolipid Adjuvant Open
Successful subunit vaccination with recombinant proteins requires adjuvants. The glycolipid trehalose-dibehenate (TDB), a synthetic analog of the mycobacterial cord factor, potently induces Th1 and Th17 immune responses and is a candidate …
View article: The Effect of Hyperthermia and Radiotherapy Sequence on Cancer Cell Death and the Immune Phenotype of Breast Cancer Cells
The Effect of Hyperthermia and Radiotherapy Sequence on Cancer Cell Death and the Immune Phenotype of Breast Cancer Cells Open
Hyperthermia (HT) is an accepted treatment for recurrent breast cancer which locally heats the tumor to 39–44 °C, and it is a very potent sensitizer for radiotherapy (RT) and chemotherapy. However, currently little is known about how HT wi…
View article: Molecular dynamics simulations of thedelta and omicron SARS-CoV-2 spike – ACE2 complexesreveal distinct changes between both variants
Molecular dynamics simulations of thedelta and omicron SARS-CoV-2 spike – ACE2 complexesreveal distinct changes between both variants Open
SARS-CoV-2, the virus which causes the COVID-19 pandemic, changes frequently through the ap-pearance of mutations constantly leading to new variants. However, only few variants evolve as dominating and will be considered as “Variants of Co…
View article: Cover Image, Volume 122, Number 12, December 2021
Cover Image, Volume 122, Number 12, December 2021 Open
Front Cover: The cover image is based on the Research Article Computational decomposition reveals reshaping of the SARS-CoV-2–ACE2 interface among viral variants expressing the N501Y mutation by Eileen Socher et al., https://doi.org/10.100…
View article: Computational decomposition reveals reshaping of the SARS‐CoV‐2–ACE2 interface among viral variants expressing the N501Y mutation
Computational decomposition reveals reshaping of the SARS‐CoV‐2–ACE2 interface among viral variants expressing the N501Y mutation Open
Variants of concern of the SARS‐CoV‐2 virus with an asparagine‐to‐tyrosine substitution at position 501 (N501Y) in the receptor‐binding domain (RBD) show enhanced infectivity compared to wild‐type, resulting in an altered pandemic situatio…
View article: Melanocytes as emerging key players in niche regulation of limbal epithelial stem cells
Melanocytes as emerging key players in niche regulation of limbal epithelial stem cells Open
These findings suggest that LMel are not only professional melanin-producing cells, but exert various non-canonical functions in limbal niche homeostasis by regulating LEPC maintenance, immune responses, and angiostasis. Their potent regul…
View article: Decomposition of the SARS-CoV-2-ACE2 interface reveals a common trend among emerging viral variants
Decomposition of the SARS-CoV-2-ACE2 interface reveals a common trend among emerging viral variants Open
New viral variants of the SARS-CoV-2 virus show enhanced infectivity compared to wild type, resulting in an altered pandemic situation in affected areas. These variants are the B.1.1.7 (United Kingdom), B.1.1.7 with the additional E484K mu…
View article: Characterization and Manipulation of the Crosstalk Between Dendritic and Natural Killer Cells Within the Tumor Microenvironment
Characterization and Manipulation of the Crosstalk Between Dendritic and Natural Killer Cells Within the Tumor Microenvironment Open
Cellular therapy has entered the daily clinical life with the approval of CAR T cell therapeutics and dendritic cell (DCs) vaccines in the US and the EU. In addition, numerous other adoptive cellular products, including natural killer (NK)…
View article: Mutations in the B.1.1.7 SARS-CoV-2 Spike Protein Reduce Receptor-Binding Affinity and Induce a Flexible Link to the Fusion Peptide
Mutations in the B.1.1.7 SARS-CoV-2 Spike Protein Reduce Receptor-Binding Affinity and Induce a Flexible Link to the Fusion Peptide Open
The B.1.1.7 variant of the SARS-CoV-2 virus shows enhanced infectiousness over the wild type virus, leading to increasing patient numbers in affected areas. Amino acid exchanges within the SARS-CoV-2 spike protein variant of B.1.1.7 affect…
View article: Inflammasomes in dendritic cells: Friend or foe?
Inflammasomes in dendritic cells: Friend or foe? Open
Inflammasomes are cytosolic multiprotein complexes that crucially contribute to host defense against pathogens but are also involved in the pathogenesis of autoinflammatory diseases. Inflammasome formation leads to activation of effector c…
View article: Mutations in the B.1.1.7 SARS-CoV-2 spike protein reduce receptor-binding affinity and induce a flexible link to the fusion peptide
Mutations in the B.1.1.7 SARS-CoV-2 spike protein reduce receptor-binding affinity and induce a flexible link to the fusion peptide Open
The B.1.1.7 variant of the SARS-CoV-2 virus shows enhanced infectiousness over the wild type virus, leading to increasing patient numbers in affected areas. A number of single amino acid exchanges and deletions within the trimeric viral sp…
View article: Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition
Maturation of Monocyte-Derived DCs Leads to Increased Cellular Stiffness, Higher Membrane Fluidity, and Changed Lipid Composition Open
Dendritic cells (DCs) are professional antigen-presenting cells of the immune system. Upon sensing pathogenic material in their environment, DCs start to mature, which includes cellular processes, such as antigen uptake, processing and pre…
View article: Subsets of CD1c+ DCs: Dendritic Cell Versus Monocyte Lineage
Subsets of CD1c+ DCs: Dendritic Cell Versus Monocyte Lineage Open
Currently three bona fide dendritic cell (DC) types are distinguished in human blood. Herein we focus on type 2 DCs (DC2s) and compare the three defining markers CD1c, CD172, and CD301. When using CD1c to define DC2s, a CD14(+)and a CD14(-…