L. Nedyalkova
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View article: A global genetic interaction map of a human cell reveals conserved principles of genetic networks
A global genetic interaction map of a human cell reveals conserved principles of genetic networks Open
We generated a genome-scale, genetic interaction network from the analysis of more than 4 million double mutants in the haploid human cell line, HAP1. The network maps ∼90,000 genetic interactions, including thousands of extreme synthetic …
View article: N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity Open
View article: N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity
N-terminal acetylation shields proteins from degradation and promotes age-dependent motility and longevity Open
SUMMARY Most eukaryotic proteins are N-terminally acetylated, but the functional impact on a global scale has remained obscure. Using genome-wide CRISPR knockout screens in human cells, we reveal a strong genetic dependency between a major…
View article: The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation
The catalytic inactivation of the N-half of human hexokinase 2 and structural and biochemical characterization of its mitochondrial conformation Open
The high proliferation rate of tumor cells demands high energy and metabolites that are sustained by a high glycolytic flux known as the ‘Warburg effect’. The activation and further metabolism of glucose is initiated by hexokinase, a focal…
View article: Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens
Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens Open
The adaptation of CRISPR/SpCas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs) targeting human protein-coding genes and encoded in viral vectors have…
View article: Evaluation and Design of Genome-wide CRISPR/Cas9 Knockout Screens
Evaluation and Design of Genome-wide CRISPR/Cas9 Knockout Screens Open
The adaptation of CRISPR/Cas9 technology to mammalian cell lines is transforming the study of human functional genomics. Pooled libraries of CRISPR guide RNAs (gRNAs), targeting human protein-coding genes and encoded in viral vectors, have…
View article: Virtual Screening for UDP-Galactopyranose Mutase Ligands Identifies a New Class of Antimycobacterial Agents
Virtual Screening for UDP-Galactopyranose Mutase Ligands Identifies a New Class of Antimycobacterial Agents Open
Galactofuranose (Galf) is present in glycans critical for the virulence and viability of several pathogenic microbes, including Mycobacterium tuberculosis, yet the monosaccharide is absent from mammalian glycans. Uridine 5'-diphosphate-gal…
View article: Colloidal Aggregation and the <i>in Vitro</i> Activity of Traditional Chinese Medicines
Colloidal Aggregation and the <i>in Vitro</i> Activity of Traditional Chinese Medicines Open
Traditional Chinese Medicines (TCMs) have been the sole source of therapeutics in China for two millennia. In recent drug discovery efforts, purified components of TCM formulations have shown activity in many in vitro assays, raising conce…