Luís Navarro
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View article: Phosphorus and sulphur affecting the growth, nutrient uptake and amino acids composition in Dunaliella salina
Phosphorus and sulphur affecting the growth, nutrient uptake and amino acids composition in Dunaliella salina Open
The potential of microalga Dunaliella salina as a protein source and in bioremediation application is still underdeveloped, despite its known carotenoids overproduction. This study focused on investigating the effects of phosphorus (P) and…
View article: Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure
Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure Open
Citrus polyploidy is associated with a wide range of morphological, genetic, and physiological changes that are often advantageous for breeding. Citrus triploid hybrids are very interesting as new seedless varieties. However, tetraploid ro…
View article: Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus
Genotyping by Sequencing for SNP-Based Linkage Analysis and the Development of KASPar Markers for Male Sterility and Polyembryony in Citrus Open
Polyembryony and male sterility (MS) are essential characters for citrus breeding. MS, coupled with parthenocarpy, allows for addressing the diversification of diploid seedless mandarin varieties, and nucleocytoplasmic MS is the most preva…
View article: Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization
Breakdown of Self-Incompatibility in Citrus by Temperature Stress, Bud Pollination and Polyploidization Open
Self-incompatibility (SI) is present in around half of all species of flowering plants. SI limits endogamy and contributes to increased genetic diversity. SI is a very important trait in citrus because, when coupled with parthenocarpy, it …
View article: Parthenocarpy and Self-Incompatibility in Mandarins
Parthenocarpy and Self-Incompatibility in Mandarins Open
Citrus reproductive biology is complex. One of its characteristic features is parthenocarpy that enables seedless fruit production. Citrus parthenocarpy and self-incompatibility knowledge is only partial and sometimes discrepant. Increasin…
View article: Alborea: A New Mid-late Mandarin Triploid Hybrid [(Citrus clementina × C. tangerina) × (C. nobilis × C. deliciosa)]
Alborea: A New Mid-late Mandarin Triploid Hybrid [(Citrus clementina × C. tangerina) × (C. nobilis × C. deliciosa)] Open
Citrus is one of the most important fruit crops worldwide, with more than 130 million tons produced in 2017. Mandarins represent 25% of this production (Food and Agriculture Organization, 2018) and are mainly destined for fresh consumption…
View article: Analysis of Crossover Events and Allele Segregation Distortion in Interspecific Citrus Hybrids by Single Pollen Genotyping
Analysis of Crossover Events and Allele Segregation Distortion in Interspecific Citrus Hybrids by Single Pollen Genotyping Open
In citrus, a classical method of studying crossovers and segregation distortion (SD) is the genetic analysis of progenies. A new strategy combining fluorescence-activated cell sorting and whole genome amplification of haploid pollen nuclei…
View article: Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks
Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks Open
Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs. This method allows combining the whole par…
View article: Origin of C. latifolia and C. aurantiifolia triploid limes: the preferential disomic inheritance of doubled-diploid ‘Mexican’ lime is consistent with an interploid hybridization hypothesis
Origin of C. latifolia and C. aurantiifolia triploid limes: the preferential disomic inheritance of doubled-diploid ‘Mexican’ lime is consistent with an interploid hybridization hypothesis Open
The doubled-diploid 'Mexican' lime had predominantly disomic segregation, producing interspecific diploid gamete structures with high C. medica/C. micrantha heterozygosity, compatible with the phylogenomic structures of triploid C. latifol…
View article: Comparative analysis of core collection sampling methods for mandarin germplasm based on molecular and phenotypic data
Comparative analysis of core collection sampling methods for mandarin germplasm based on molecular and phenotypic data Open
Gene banks have been established to conserve the genetic diversity of crop species. Large germplasm collections lead to management problems (space, maintenance costs, etc.), especially in collections involving species with recalcitrant see…
View article: Unreduced Megagametophyte Production in Lemon Occurs via Three Meiotic Mechanisms, Predominantly Second-Division Restitution
Unreduced Megagametophyte Production in Lemon Occurs via Three Meiotic Mechanisms, Predominantly Second-Division Restitution Open
Unreduced (2n) gametes have played a pivotal role in polyploid plant evolution and are useful for sexual polyploid breeding in various species, particularly for developing new seedless citrus varieties. The underlying mechanisms of 2n game…
View article: Changes in Anthocyanin Production during Domestication of <i>Citrus</i>
Changes in Anthocyanin Production during Domestication of <i>Citrus</i> Open
Mandarin (Citrus reticulata), citron (Citrus medica), and pummelo (Citrus maxima) are important species of the genus Citrus and parents of the interspecific hybrids that constitute the most familiar commercial varieties of Citrus: sweet or…
View article: Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection
Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection Open
Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group with…
View article: Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR Open
We validated the concept that with well-established clades, DSNPs can be selected and efficiently transformed into competitive allele-specific PCR markers (KASPar method) allowing cost-effective highly efficient cladistic analysis in large…
View article: Tetraploidy Enhances Boron-Excess Tolerance in Carrizo Citrange (Citrus sinensis L. Osb. × Poncirus trifoliata L. Raf.)
Tetraploidy Enhances Boron-Excess Tolerance in Carrizo Citrange (Citrus sinensis L. Osb. × Poncirus trifoliata L. Raf.) Open
Tetraploidy enhances B excess tolerance in citrange CarrizoExpression of NIP5 and BOR1 transporters and cell wall-bounded B are similar between ploidiesB tolerance is attributed to root anatomical modifications induced by genome duplicatio…
View article: Phylogenetic origin of limes and lemons revealed by cytoplasmic and nuclear markers
Phylogenetic origin of limes and lemons revealed by cytoplasmic and nuclear markers Open
Citrus medica contributed to all limes and lemons and was the direct male parent for the main sub-groups in combination with C. micrantha or close papeda species (for C. aurata, C. excelsa, C. macrophylla and C. aurantifolia--'Mexican' lim…
View article: Precocious flowering of juvenile citrus induced by a viral vector based on <i>Citrus leaf blotch virus</i>: a new tool for genetics and breeding
Precocious flowering of juvenile citrus induced by a viral vector based on <i>Citrus leaf blotch virus</i>: a new tool for genetics and breeding Open
Summary The long juvenile period of citrus trees (often more than 6 years) has hindered genetic improvement by traditional breeding methods and genetic studies. In this work, we have developed a biotechnology tool to promote transition fro…
View article: Additional file 1: of Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
Additional file 1: of Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR Open
Sequence accession (GenBank) used for SNP in silico mining and their classification in the Bayer et al. [21] analysis. (XLSX 20Â kb)
View article: Additional file 4: of Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR
Additional file 4: of Towards a molecular taxonomic key of the Aurantioideae subfamily using chloroplastic SNP diagnostic markers of the main clades genotyped by competitive allele-specific PCR Open
Example of diagnostic SNP mining in the region rps4-trnT in the position 49301 distinguishing the accessions of clade Q (A) from all other Aurantioideae accessions (G). (XLSX 38Â kb)