José Luis González Montesinos
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View article: Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory
Continuous Process Verification 4.0 application in upstream: adaptiveness implementation managed by AI in the hypoxic bioprocess of the Pichia pastoris cell factory Open
The experimental approach developed in this research demonstrated how the cloud, the Internet of Things (IoT), edge computing, and Artificial Intelligence (AI), considered key technologies in Industry 4.0, provide the expected horizon for …
View article: Synergic kinetic and physiological control to improve the efficiency of <i>Komagataella phaffii</i> recombinant protein production bioprocesses
Synergic kinetic and physiological control to improve the efficiency of <i>Komagataella phaffii</i> recombinant protein production bioprocesses Open
The yeast Komagataella phaffii ( Pichia pastoris ) is currently considered a versatile and highly efficient host for recombinant protein production ( RPP ). Interestingly, the regulated application of specific stress factors as part of bio…
View article: Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production Open
The combination of strain and bioprocess engineering strategies should be considered to obtain the highest levels of recombinant protein production (RPP) while assuring product quality and process reproducibility of heterologous products. …
View article: Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9
Scalable production and application of Pichia pastoris whole cell catalysts expressing human cytochrome P450 2C9 Open
Background Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. Accelerating the drug development p…
View article: Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory Open
The methylotrophic yeast Pichia pastoris (Komagataella phaffii) is currently considered one of the most promising hosts for recombinant protein production (RPP) and metabolites due to the availability of several tools to efficiently regula…
View article: Rationale‐based selection of optimal operating strategies and gene dosage impact on recombinant protein production in <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> )
Rationale‐based selection of optimal operating strategies and gene dosage impact on recombinant protein production in <i>Komagataella phaffii</i> ( <i>Pichia pastoris</i> ) Open
Summary Its features as a microbial and eukaryotic organism have turned Komagataella phaffii ( Pichia pastoris ) into an emerging cell factory for recombinant protein production (RPP). As a key step of the bioprocess development, this work…
View article: MOESM1 of Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage
MOESM1 of Specific growth rate governs AOX1 gene expression, affecting the production kinetics of Pichia pastoris (Komagataella phaffii) PAOX1-driven recombinant producer strains with different target gene dosage Open
Additional file 1. List of primers used for qPCR analyses.
View article: Physiological state as transferable operating criterion to improve recombinant protein production in <i>Pichia pastoris</i> through oxygen limitation
Physiological state as transferable operating criterion to improve recombinant protein production in <i>Pichia pastoris</i> through oxygen limitation Open
BACKGROUND The yeast Pichia pastoris is widely used as a production platform for secreted recombinant protein. The application of oxygen‐limiting conditions leads to an important increase in protein specific productivity driven by the GAP …
View article: Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris
Overall Key Performance Indicator to Optimizing Operation of High-Pressure Homogenizers for a Reliable Quantification of Intracellular Components in Pichia pastoris Open
The most commonly used cell disruption procedures may present lack of reproducibility, which introduces significant errors in the quantification of intracellular components. In this work, an approach consisting in the definition of an over…