Lacey Johnson
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View article: Cold-stored platelets: revisiting assumptions and addressing variability to support implementation
Cold-stored platelets: revisiting assumptions and addressing variability to support implementation Open
Platelets are transfused to patients to prevent and stop bleeding. Conventionally, platelets are stored at room temperature (RT; 20–24 °C), however, this limits their shelf life to 5–7 days, due to an increased risk of bacterial proliferat…
View article: Cryopreserved vs Liquid-Stored Platelets for the Treatment of Surgical Bleeding
Cryopreserved vs Liquid-Stored Platelets for the Treatment of Surgical Bleeding Open
Importance Liquid-stored platelets have a shelf-life of 5 to 7 days, limiting availability and resulting in wastage. Objective To assess the effectiveness and safety of dimethyl sulfoxide–cryopreserved platelets, which have a shelf-life of…
View article: Making cold platelets even cooler: Freezing cold‐stored platelets to maximize inventory potential
Making cold platelets even cooler: Freezing cold‐stored platelets to maximize inventory potential Open
Background and Objectives Refrigeration and cryopreservation are attractive alternatives that could support an extension of the current room temperature shelf life of platelets. As transfusion of cold‐stored platelets (CSP) is currently li…
View article: Preparation of cold‐stored platelets on‐demand: A novel approach to inventory management
Preparation of cold‐stored platelets on‐demand: A novel approach to inventory management Open
Background Cold‐stored platelets (CSP) are now being used to treat acute bleeding. However, as CSP are less suitable for prophylaxis, both room temperature (RT) platelets and CSP will be required, which complicates inventory management. Th…
View article: Characterization of the in vitro quality parameters of cold‐stored platelets containing aggregates
Characterization of the in vitro quality parameters of cold‐stored platelets containing aggregates Open
Background and Objectives Cold‐stored platelets (CSP) are being used more widely for the treatment of bleeding because of their longer shelf life. However, storage at low temperatures can induce the formation of platelet aggregates. We aim…
View article: Clot formation and resolution properties of platelets during cold storage for 21 days
Clot formation and resolution properties of platelets during cold storage for 21 days Open
Background Cold storage (2–6°C) preserves the aggregatory and clot formation properties of platelets beyond the standard 7‐day shelf life. Comparatively little is known about the effect of cold storage on clot resolution functions, which a…
View article: Storage Temperature Affects Platelet Activation and Degranulation in Response to Stimuli
Storage Temperature Affects Platelet Activation and Degranulation in Response to Stimuli Open
The refrigeration (cold storage) of platelet components provides several benefits over room-temperature (RT) storage, extending the shelf-life up to 21 days. However, the effect of storage conditions on platelet activation in response to s…
View article: Lipidomic changes occurring in platelets during extended cold storage
Lipidomic changes occurring in platelets during extended cold storage Open
Objectives Cold storage is being implemented as an alternative to conventional room‐temperature storage for extending the shelf‐life of platelet components beyond 5–7 days. The aim of this study was to characterise the lipid profile of pla…
View article: The phenotype of cryopreserved platelets influences the formation of platelet-leukocyte aggregates in an <i>in vitro</i> model
The phenotype of cryopreserved platelets influences the formation of platelet-leukocyte aggregates in an <i>in vitro</i> model Open
Cryopreservation significantly alters the phenotype of platelets; generating distinct subpopulations, which may influence the formation of platelet leukocyte aggregates (PLA). PLAs are immunomodulatory and have been associated with transfu…
View article: The role of sodium citrate during extended cold storage of platelets in platelet additive solutions
The role of sodium citrate during extended cold storage of platelets in platelet additive solutions Open
Background Cold‐stored platelets are increasingly being used to treat bleeding. Differences in manufacturing processes and storage solutions can affect platelet quality and may influence the shelf life of cold‐stored platelets. PAS‐E and P…
View article: Identification of platelet subpopulations in cryopreserved platelet components using multi-colour imaging flow cytometry
Identification of platelet subpopulations in cryopreserved platelet components using multi-colour imaging flow cytometry Open
Cryopreservation of platelets, at − 80 °C with 5–6% DMSO, results in externalisation of phosphatidylserine and the formation of extracellular vesicles (EVs), which may mediate their procoagulant function. The phenotypic features of procoag…
View article: Cryopreserved platelets compared with liquid-stored platelets for the treatment of surgical bleeding: protocol for two multicentre randomised controlled blinded non-inferiority trials (the CLIP-II and CLIPNZ-II trials)
Cryopreserved platelets compared with liquid-stored platelets for the treatment of surgical bleeding: protocol for two multicentre randomised controlled blinded non-inferiority trials (the CLIP-II and CLIPNZ-II trials) Open
Introduction Cryopreservation at −80°C in dimethylsulphoxide extends platelet shelf-life from 7 days to 2 years. Only limited comparative trial data supports the safety and effectiveness of cryopreserved platelets as a treatment for surgic…
View article: Gamma and X‐ray irradiation do not affect the in vitro quality of refrigerated apheresis platelets in platelet additive solution (<scp>PAS‐E</scp>)
Gamma and X‐ray irradiation do not affect the in vitro quality of refrigerated apheresis platelets in platelet additive solution (<span>PAS‐E</span>) Open
Background Platelet refrigeration (cold storage) provides the advantages of an extended shelf life and reduces the risk of bacterial growth, compared to platelets stored at room temperature (RT). However, processing modifications, such as …
View article: Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride
Effect of Recombinant Human Perlecan Domain V Tethering Method on Protein Orientation and Blood Contacting Activity on Polyvinyl Chloride Open
Surface modification of biomaterials is a promising approach to control biofunctionality while retaining the bulk biomaterial properties. Perlecan is the major proteoglycan in the vascular basement membrane that supports low levels of plat…
View article: The immune potential of <i>ex vivo</i> stored platelets: a review
The immune potential of <i>ex vivo</i> stored platelets: a review Open
Platelets are now acknowledged as key regulators of the immune system, as they are capable of mediating inflammation, leucocyte recruitment and activation. This activity is facilitated through platelet activation, which induces significant…
View article: Comparison between manufacturing sites shows differential adhesion, activation, and GPIbα expression of cryopreserved platelets
Comparison between manufacturing sites shows differential adhesion, activation, and GPIbα expression of cryopreserved platelets Open
BACKGROUND Transfusion of cryopreserved platelets (cryoplatelets) is not common but may replace standard liquid‐preserved platelets (PLTs) in specific circumstances. To better understand cryoplatelet function, frozen concentrates from diff…
View article: Ultraviolet-Based Pathogen Inactivation Systems: Untangling the Molecular Targets Activated in Platelets
Ultraviolet-Based Pathogen Inactivation Systems: Untangling the Molecular Targets Activated in Platelets Open
Transfusions of platelets are an important cornerstone of medicine; however, recipients may be subject to risk of adverse events associated with the potential transmission of pathogens, especially bacteria. Pathogen inactivation (PI) techn…
View article: Refrigeration, cryopreservation and pathogen inactivation: an updated perspective on platelet storage conditions
Refrigeration, cryopreservation and pathogen inactivation: an updated perspective on platelet storage conditions Open
Conventional storage of platelet concentrates limits their shelf life to between 5 and 7 days due to the risk of bacterial proliferation and the development of the platelet storage lesion. Cold storage and cryopreservation of platelets may…
View article: Perspectives on the use of biomaterials to store platelets for transfusion
Perspectives on the use of biomaterials to store platelets for transfusion Open
Platelets are routinely stored enabling transfusions for a range of conditions. While the current platelet storage bags, composed of either polyvinylchloride or polyolefin, are well-established, the storage of platelets in these bags beyon…