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View article: Definition of regulatory elements and transcription factors controlling porcine immune cell gene expression at single cell resolution using single nucleus ATAC-seq
Definition of regulatory elements and transcription factors controlling porcine immune cell gene expression at single cell resolution using single nucleus ATAC-seq Open
The transcriptome of porcine peripheral blood mononuclear cells (PBMC) at single cell (sc) resolution is well described, but little is understood about the cis-regulatory mechanism behind scPBMC gene expression. Here, we profiled the open …
View article: Characterization of double humanized BLT-mice with stable engraftment of a human gut bacterial microbiome
Characterization of double humanized BLT-mice with stable engraftment of a human gut bacterial microbiome Open
Humanized mice with human-like immune systems are commonly used to study immune responses to human-specific pathogens. However, one limitation of using humanized mice is their native murine gut microbiota, which significantly differs from …
View article: Definition of regulatory elements and transcription factors controlling immune cell gene expression at single cell resolution using single nucleus ATAC-seq
Definition of regulatory elements and transcription factors controlling immune cell gene expression at single cell resolution using single nucleus ATAC-seq Open
The transcriptome of porcine peripheral blood mononuclear cells (PBMC) at single cell (sc) resolution is well described, but little is understood about the cis-regulatory mechanism behind scPBMC gene expression. Here, we profiled the open …
View article: Definition of Regulatory Elements and Transcription Factors Controlling Immune Cell Gene Expression at Single Cell Resolution Using Single Nucleus ATAC-SEQ
Definition of Regulatory Elements and Transcription Factors Controlling Immune Cell Gene Expression at Single Cell Resolution Using Single Nucleus ATAC-SEQ Open
View article: PSII-6 Computational Tools and Resources for Analysis and Exploration of Single-Cell Rnaseq Data in Agriculture
PSII-6 Computational Tools and Resources for Analysis and Exploration of Single-Cell Rnaseq Data in Agriculture Open
The agriculture genomics community has numerous data submission standards available but little experience describing and storing single-cell (e.g., scRNAseq) data. Other single-cell genomics infrastructure efforts, such as the Human Cell A…
View article: Integrative profiling of gene expression and chromatin accessibility elucidates specific transcriptional networks in porcine neutrophils
Integrative profiling of gene expression and chromatin accessibility elucidates specific transcriptional networks in porcine neutrophils Open
Neutrophils are vital components of the immune system for limiting the invasion and proliferation of pathogens in the body. Surprisingly, the functional annotation of porcine neutrophils is still limited. The transcriptomic and epigenetic …
View article: Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing
Reference Transcriptomes of Porcine Peripheral Immune Cells Created Through Bulk and Single-Cell RNA Sequencing Open
Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight…
View article: Reference transcriptomes of porcine peripheral immune cells created through bulk and single-cell RNA sequencing
Reference transcriptomes of porcine peripheral immune cells created through bulk and single-cell RNA sequencing Open
Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight…
View article: Alterations in the gut microbiome by HIV-1 infection or a high-fat diet associates with systemic immune activation and inflammation in double humanized-BLT mice
Alterations in the gut microbiome by HIV-1 infection or a high-fat diet associates with systemic immune activation and inflammation in double humanized-BLT mice Open
Background While the translatability of gut microbiome studies utilizing animal models to humans has proven difficult, studying the gut microbiome directly in humans is also challenging due to the existence of many confounding variables. T…
View article: A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System Open
Humanized mice (hu-mice) that feature a functional human immune system have fundamentally changed the study of human pathogens and disease. They can be used to model diseases that are otherwise difficult or impossible to study in humans or…
View article: Stable Engraftment of a Human Gut Bacterial Microbiome in Double Humanized BLT-mice
Stable Engraftment of a Human Gut Bacterial Microbiome in Double Humanized BLT-mice Open
Background Humanized mice featuring a functional human immune system are an important pre-clinical model for examining immune responses to human-specific pathogens. This model has been widely utilized to study human diseases that are other…
View article: SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model
SIVcpz closely related to the ancestral HIV-1 is less or non-pathogenic to humans in a hu-BLT mouse model Open
The HIV-1 pandemic is a consequence of the cross-species transmission of simian immunodeficiency virus in wild chimpanzees (SIVcpz) to humans. Our previous study demonstrated SIVcpz strains that are closely related to the ancestral viruses…
View article: Identification of Unequally Represented Founder Viruses Among Tissues in Very Early SIV Rectal Transmission
Identification of Unequally Represented Founder Viruses Among Tissues in Very Early SIV Rectal Transmission Open
Characterizing the transmitted/founder (T/F) viruses of multi-variant SIV infection may shed new light on the understanding of mucosal transmission. We intrarectally inoculated six Chinese rhesus macaques with a single high dose of SIVmac2…
View article: A DNA Vaccine Expressing Consensus Hemagglutinin-Esterase Fusion Protein Protected Guinea Pigs from Infection by Two Lineages of Influenza D Virus
A DNA Vaccine Expressing Consensus Hemagglutinin-Esterase Fusion Protein Protected Guinea Pigs from Infection by Two Lineages of Influenza D Virus Open
Two lineages of influenza D virus (IDV) have been found to infect cattle and promote bovine respiratory disease complex, one of the most commonly diagnosed causes of morbidity and mortality within the cattle industry. Furthermore, IDV can …