Lara Hauke
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View article: SarcAsM: AI-based multiscale analysis of sarcomere organization and contractility in cardiomyocytes
SarcAsM: AI-based multiscale analysis of sarcomere organization and contractility in cardiomyocytes Open
Cardiomyocyte function critically depends on sarcomere dynamics and their organization in myofibrils. To uncover how cardiomyocyte function emerges from individual sarcomere dynamics, comprehensive analysis of Z-bands (nanometer scale), sa…
View article: Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness
Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness Open
Cardiac muscle function emerges from the coordinated contraction of sarcomeres in cardiomyocytes. Sarcomere dynamics are usually inferred from whole-cell or myofibril observations, assuming synchronized, uniform behavior. Here, we investig…
View article: Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness
Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness Open
Cardiac muscle function emerges from the coordinated contraction of sarcomeres in cardiomyocytes. Sarcomere dynamics are usually inferred from whole-cell or myofibril observations, assuming synchronized, uniform behavior. Here, we investig…
View article: Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness
Stochastic tug-of-war among sarcomeres mediates cardiomyocyte response to environmental stiffness Open
Cardiac muscle function emerges from the coordinated contraction of sarcomeres in cardiomyocytes. Sarcomere dynamics are usually inferred from whole-cell or myofibril observations, assuming synchronized, uniform behavior. Here, we investig…
View article: Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT
Three-dimensional multi-target super-resolution microscopy of cells using Metal-Induced Energy Transfer and DNA-PAINT Open
Absrtact Achieving nanometer precision in 3D remains a major challenge in super-resolution microscopy. DNA-PAINT offers excellent lateral resolution and versatile multiplexing, but its axial localization precision is typically 3–5 times po…
View article: DNA-Based Optical Sensors for Forces in Cytoskeletal Networks
DNA-Based Optical Sensors for Forces in Cytoskeletal Networks Open
Mechanical forces are relevant for many biological processes, from wound healing and tumor formation to cell migration and differentiation. Cytoskeletal actin is largely responsible for responding to forces and transmitting them in cells, …
View article: Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins
Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins Open
Metal-induced energy transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of a microscope. Although its capability in numerous biological and biophysical studie…
View article: FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking
FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking Open
Cytoskeletal pattern formation and structural dynamics are key to a variety of biological functions and a detailed and quantitative analysis yields insight into finely tuned and well-balanced homeostasis and potential pathological alterati…
View article: Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins
Metal-Induced Energy Transfer (MIET) for Live-Cell Imaging with Fluorescent Proteins Open
Metal-Induced Energy Transfer (MIET) imaging is an easy-to-implement super-resolution modality that achieves nanometer resolution along the optical axis of a microscope. Although its capability in numerous biological and biophysical studie…
View article: FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking
FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking Open
This is the software described in our article 'FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking' and the used datasets for image analysis. It is intended as a easy to use software for tracking of …
View article: FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking
FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking Open
This is the software described in our article 'FilamentSensor 2.0: An open-source modular toolbox for 2D/3D cytoskeletal filament tracking' and the used datasets for image analysis. It is intended as a easy to use software for tracking of …
View article: A DNA-based optical force sensor for live-cell applications
A DNA-based optical force sensor for live-cell applications Open
Mechanical forces are relevant for many biological processes, from wound healing or tumour formation to cell migration and differentiation. Cytoskeletal actin is largely responsible for responding to forces and transmitting them in cells, …
View article: A Focal Adhesion Filament Cross-correlation Kit for fast, automated segmentation and correlation of focal adhesions and actin stress fibers in cells
A Focal Adhesion Filament Cross-correlation Kit for fast, automated segmentation and correlation of focal adhesions and actin stress fibers in cells Open
Focal adhesions (FAs) and associated actin stress fibers (SFs) form a complex mechanical system that mediates bidirectional interactions between cells and their environment. This linked network is essential for mechanosensing, force produc…
View article: A Focal Adhesion Filament Cross-correlation Kit for fast, automated segmentation and correlation of focal adhesions and actin stress fibers in cells
A Focal Adhesion Filament Cross-correlation Kit for fast, automated segmentation and correlation of focal adhesions and actin stress fibers in cells Open
Focal adhesions (FAs) and associated actin stress fibers (SFs) form a complex mechanical system that mediates bidirectional interactions between cells and their environment. This linked network is essential for mechanosensing, force produc…
View article: Metasurface-based total internal reflection microscopy
Metasurface-based total internal reflection microscopy Open
Recent years have seen a tremendous progress in the development of dielectric metasurfaces for visible light applications. Such metasurfaces are ultra-thin optical devices that can manipulate optical wavefronts in an arbitrary manner. Here…
View article: A Statistical and Biophysical Toolbox to Elucidate Structure and Formation of Stress Fibers
A Statistical and Biophysical Toolbox to Elucidate Structure and Formation of Stress Fibers Open
We are concerned with statistically validated early mechanically guided differentiation of human mesenchymal stem cells (hMSCs). This chapter reviews and extends methods of fixed and live imaging of hMSCs, automated reliable and unbiased n…
View article: Multi-parameter assessment of mechano-sensitivity driven differentiation of human mesenchymal stem cells
Multi-parameter assessment of mechano-sensitivity driven differentiation of human mesenchymal stem cells Open
What factors and conditions control stem cell differentiation is currently one of the major questions in
\nbiomedical research. What factors ultimately influence the cells’ decision is unclear but it has been shown,
\nthat stem cells diffe…
View article: Cellular and Molecular Effects of High-Molecular-Weight Heparin on Matrix Metalloproteinase 9 Expression
Cellular and Molecular Effects of High-Molecular-Weight Heparin on Matrix Metalloproteinase 9 Expression Open
Blood sampling with different anticoagulants alters matrix metalloproteinase (MMP-) 9 expression, thus influencing its concentration and diagnostic validity. Here, we aimed to evaluate the effects of different anticoagulants on MMP-9 regul…
View article: Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy
Dual-color metal-induced and Förster resonance energy transfer for cell nanoscopy Open
We report a novel method, dual-color axial nanometric localization by metal-induced energy transfer, and combine it with Förster resonance energy transfer (FRET) for resolving structural details in cells on the molecular level. We demonst…