Lars G. Kamphuis
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View article: Genetic mapping of Ascochyta blight resistance in an ILL6002 × Indianhead lentil mapping population
Genetic mapping of Ascochyta blight resistance in an ILL6002 × Indianhead lentil mapping population Open
Ascochyta blight of lentil ( Lens culinaris Medik.) is a fungal disease caused by Ascochyta lentis . This study was carried out to identify the location of quantitative trait loci (QTL) associated with resistance from the accession Indianh…
View article: Boscalid – a new selectable marker for Ascochyta lentis and Ascochyta rabiei
Boscalid – a new selectable marker for Ascochyta lentis and Ascochyta rabiei Open
Ascochyta lentis and Ascochyta rabiei are fungal pathogens affecting lentil and chickpea crops, respectively. They employ effector proteins to facilitate infection, and understanding the role of effector genes is crucial for unravelling ho…
View article: Recombination and transposition drive genomic structural variation potentially impacting life history traits in a host-generalist fungal plant pathogen
Recombination and transposition drive genomic structural variation potentially impacting life history traits in a host-generalist fungal plant pathogen Open
Background An understanding of plant pathogen evolution is important for sustainable management of crop diseases. Plant pathogen populations must maintain adequate heritable phenotypic variability to survive. Polymorphisms ≥ 50 bp, known a…
View article: The History and Pedigree of Australian Lentil Cultivars
The History and Pedigree of Australian Lentil Cultivars Open
Lentils are an ancient, edible grain legume, consumed worldwide in an array of dishes as either whole or split seed. While India and Canada are the largest modern‐day producers, Australia is a close third and the second largest exporter of…
View article: Evaluation of alkaloid levels in commercial and wild genotypes of narrow-leafed lupin
Evaluation of alkaloid levels in commercial and wild genotypes of narrow-leafed lupin Open
Lupins are a promising source of protein that can complement soybean-based proteins in food and animal feed. Narrow-leafed lupin (Lupinus angustifolius) is the predominant lupin species cultivated in Australia and Europe. Consumption of lu…
View article: Cryptic recombination and transposition drive structural variation to shape genomic plasticity and life history traits in a host generalist fungal plant pathogen
Cryptic recombination and transposition drive structural variation to shape genomic plasticity and life history traits in a host generalist fungal plant pathogen Open
Background An understanding of plant pathogen evolution is important for sustainable management of crop diseases. Plant pathogen populations must maintain adequate heritable phenotypic variability to survive. Polymorphisms >= 50 bp, known …
View article: Genetic dissection of domestication traits in interspecific chickpea populations
Genetic dissection of domestication traits in interspecific chickpea populations Open
Chickpea ( Cicer arietinum ) is a pulse crop that provides an integral source of nutrition for human consumption. The close wild relatives Cicer reticulatum and Cicer echinospermum harbor untapped genetic diversity that can be exploited by…
View article: Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets
Genome-wide identification of Sclerotinia sclerotiorum small RNAs and their endogenous targets Open
Background Several phytopathogens produce small non-coding RNAs of approximately 18–30 nucleotides (nt) which post-transcriptionally regulate gene expression. Commonly called small RNAs (sRNAs), these small molecules were also reported to …
View article: The broad host range pathogen <i>Sclerotinia sclerotiorum</i> produces multiple effector proteins that induce host cell death intracellularly
The broad host range pathogen <i>Sclerotinia sclerotiorum</i> produces multiple effector proteins that induce host cell death intracellularly Open
Sclerotinia sclerotiorum is a broad host range necrotrophic fungal pathogen, which causes disease on many economically important crop species. S. sclerotiorum has been shown to secrete small effector proteins to kill host cells and acquire…
View article: A pan‐genome and chromosome‐length reference genome of narrow‐leafed lupin (<i>Lupinus angustifolius</i>) reveals genomic diversity and insights into key industry and biological traits
A pan‐genome and chromosome‐length reference genome of narrow‐leafed lupin (<i>Lupinus angustifolius</i>) reveals genomic diversity and insights into key industry and biological traits Open
SUMMARY Narrow‐leafed lupin (NLL; Lupinus angustifolius ) is a key rotational crop for sustainable farming systems, whose grain is high in protein content. It is a gluten‐free, non‐genetically modified, alternative protein source to soybea…
View article: Identification of Sclerotinia stem rot resistance quantitative trait loci in a chickpea (Cicer arietinum) recombinant inbred line population
Identification of Sclerotinia stem rot resistance quantitative trait loci in a chickpea (Cicer arietinum) recombinant inbred line population Open
Sclerotinia stem rot (SSR), caused by Sclerotinia sclerotiorum, is one of the most economically devastating diseases in chickpea (Cicer arietinum L.). No complete resistance is available in chickpea to this disease, and the inheritance of …
View article: The novel avirulence effector AlAvr1 from <i>Ascochyta lentis</i> mediates host cultivar specificity of ascochyta blight in lentil
The novel avirulence effector AlAvr1 from <i>Ascochyta lentis</i> mediates host cultivar specificity of ascochyta blight in lentil Open
Ascochyta lentis is a fungal pathogen that causes ascochyta blight in the important grain legume species lentil, but little is known about the molecular mechanism of disease or host specificity. We employed a map‐based cloning approach usi…
View article: A multiplex PCR marker distinguishes between a series of four <i>LanFTc1</i> alleles regulating flowering time in narrow‐leafed lupin (<scp><i>Lupinus angustifolius</i></scp>)
A multiplex PCR marker distinguishes between a series of four <i>LanFTc1</i> alleles regulating flowering time in narrow‐leafed lupin (<span><i>Lupinus angustifolius</i></span>) Open
We designed and validated a new multiplex PCR marker which discriminates between four insertion/deletion (INDEL) alleles in the 5′ regulatory region of a major flowering time gene in Lupinus angustifolius , LanFTc1 . The four INDEL alleles…
View article: Genomic resources for lupins are coming of age
Genomic resources for lupins are coming of age Open
Lupins are underutilised pulse crops subject to increasing interest for human consumption of the high‐protein grain. They are also valued as a source of animal nutrition and make an excellent break crop in agricultural production systems. …
View article: Modeling first order additive × additive epistasis improves accuracy of genomic prediction for sclerotinia stem rot resistance in canola
Modeling first order additive × additive epistasis improves accuracy of genomic prediction for sclerotinia stem rot resistance in canola Open
The fungus Sclerotinia sclerotiorum infects hundreds of plant species including many crops. Resistance to this pathogen in canola ( Brassica napus L. subsp. napus ) is controlled by numerous quantitative trait loci (QTL). For such polygeni…
View article: Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines
Analysis of Differentially Expressed Sclerotinia Sclerotiorum Genes During the Interaction With Moderately Resistant and Highly Susceptible Chickpea Lines Open
Background : Sclerotinia sclerotiorum , the cause of Sclerotinia stem rot (SSR), is a host generalist necrotrophic fungus that can cause major yield losses in chickpea ( Cicer arietinum ) production. This study used RNA sequencing to condu…
View article: Additional file 7 of Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 7 of Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 7: Supplementary Table 4. Genes targeted by conserved miRNAs only in the infected sample.
View article: Additional file 12 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 12 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 12: Supplementary Table 9. Primers used in this study.
View article: Additional file 4 of Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 4 of Identification of Brassica napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 4: Supplementary Table 1. All small RNA biogenesis loci identified using the program Sho:rtStack.
View article: Additional file 10 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 10 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 10: Supplementary Table 7. GO term enrichment analysis of 5918 transcripts possibly regulated by pha-siRNAs based on psRNA target analysis.
View article: Additional file 5 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 5 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 5: Supplementary Table 2. Cleaved products of the 73 miRNA families. All targets identified using degradome sequencing in infected and mock samples are included.
View article: Additional file 9 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen
Additional file 9 of fIdentification of B. napus small RNAs responsive to infection by a necrotrophic pathogen Open
Additional file 9: Supplementary Table 6. Known and novel miRNAs identified in this study.