Lars Lilge
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View article: Die Erweiterung des genetischen Codes als molekulare Prozesskontrolle
Die Erweiterung des genetischen Codes als molekulare Prozesskontrolle Open
Bioproduction of bioactive compounds on a large-scale poses challenges that require molecular strain engineering. By integrating the principle of genetic code expansion (GCE), the biosynthesis of the surfactin biosurfactant has been contro…
View article: Glucoselipid Biosurfactant Biosynthesis Operon of Rouxiella badensis DSM 100043T: Screening, Identification, and Heterologous Expression in Escherichia coli
Glucoselipid Biosurfactant Biosynthesis Operon of Rouxiella badensis DSM 100043T: Screening, Identification, and Heterologous Expression in Escherichia coli Open
Rouxiella badensis DSM 100043T had been previously proven to produce a novel glucoselipid biosurfactant which has a very low critical micelle concentration (CMC) as well as very good stability against a wide range of pH, temperature, and s…
View article: Genetic Code Expansion for Controlled Surfactin Production in a High Cell-Density Bacillus subtilis Strain
Genetic Code Expansion for Controlled Surfactin Production in a High Cell-Density Bacillus subtilis Strain Open
Background: In biotechnology, B. subtilis is established for heterologous protein production. In addition, the species provides a variety of bioactive metabolites, including the non-ribosomally produced surfactin lipopeptide. However, to c…
View article: Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440
Bioprocess exploitation of microaerobic auto-induction using the example of rhamnolipid biosynthesis in Pseudomonas putida KT2440 Open
Background In biomanufacturing of surface-active agents, such as rhamnolipids, excessive foaming is a significant obstacle for the development of high-performing bioprocesses. The exploitation of the inherent tolerance of Pseudomonas putid…
View article: Recombinant Production of Bovine αS1-Casein in Genome-Reduced Bacillus subtilis Strain IIG-Bs-20-5-1
Recombinant Production of Bovine αS1-Casein in Genome-Reduced Bacillus subtilis Strain IIG-Bs-20-5-1 Open
Background: Cow’s milk represents an important protein source. Here, especially casein proteins are important components, which might be a promising source of alternative protein production by microbial expression systems. Nevertheless, ca…
View article: In Vivo Quantification of Surfactin Nonribosomal Peptide Synthetase Complexes in Bacillus subtilis
In Vivo Quantification of Surfactin Nonribosomal Peptide Synthetase Complexes in Bacillus subtilis Open
Surfactin, a potent biosurfactant produced by Bacillus subtilis, is synthesized using a non-ribosomal peptide synthetase (NRPS) encoded by the srfAA-AD operon. Despite its association with quorum sensing via the ComX pheromone, the dynamic…
View article: The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes
The influence of growth rate-controlling feeding strategy on the surfactin production in Bacillus subtilis bioreactor processes Open
Background The production of surfactin, an extracellular accumulating lipopeptide produced by various Bacillus species, is a well-known representative of microbial biosurfactant. However, only limited information is available on the correl…
View article: Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments
Toward Effects of Hydrophobicity on Biosurfactant Production by Bacillus subtilis Isolates from Crude-Oil-Exposed Environments Open
Background: Due to their structural features, biosurfactants reveal promising physicochemical properties, making them interesting for various applications in different fields, such as the food, cosmetics, agriculture, and bioremediation se…
View article: Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans
Lysinibacillus irui sp. nov., isolated from Iru, fermented African locust beans Open
A Gram-positive, motile, aerobic, rod-shaped, endospore-forming strain designated IRB4-01 T was isolated from fermented African locust beans (Iru) obtained from Bodija market in the city of Ibadan, southwestern Nigeria, during a screening …
View article: Evaluation of an external foam column for in situ product removal in aerated surfactin production processes
Evaluation of an external foam column for in situ product removal in aerated surfactin production processes Open
In Bacillus fermentation processes, severe foam formation may occur in aerated bioreactor systems caused by surface-active lipopeptides. Although they represent interesting compounds for industrial biotechnology, their property of foaming …
View article: Characterization ofantifungal properties of lipopeptide-producing Bacillus velezensis strains and their proteome-based response to the phytopathogens, Diaporthe spp
Characterization ofantifungal properties of lipopeptide-producing Bacillus velezensis strains and their proteome-based response to the phytopathogens, Diaporthe spp Open
Introduction: B. velezensis strains are of interest in agricultural applications due to their beneficial interactions with plants, notable through their antimicrobial activity. The biocontrol ability of two new lipopeptides-producing B. ve…
View article: Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications
Design and evaluation of a 3D‐printed, lab‐scale perfusion bioreactor for novel biotechnological applications Open
3D‐printing increased in significance for biotechnological research as new applications like lab‐on‐a‐chip systems, cell culture devices or 3D‐printed foods were uncovered. Besides mammalian cell culture, only few of those applications foc…
View article: Characterization of Bacillus velezensis UTB96, Demonstrating Improved Lipopeptide Production Compared to the Strain B. velezensis FZB42
Characterization of Bacillus velezensis UTB96, Demonstrating Improved Lipopeptide Production Compared to the Strain B. velezensis FZB42 Open
Bacillus strains can produce various lipopeptides, known for their antifungal properties. This makes them attractive metabolites for applications in agriculture. Therefore, identification of productive wild-type strains is essential for th…
View article: High‐level recombinant protein production with <i>Corynebacterium glutamicum</i> using acetate as carbon source
High‐level recombinant protein production with <i>Corynebacterium glutamicum</i> using acetate as carbon source Open
In recent years, biotechnological conversion of the alternative carbon source acetate has attracted much attention. So far, acetate has been mainly used for microbial production of bioproducts with bulk applications. In this study, we aime…
View article: Exploration of surfactin production by newly isolated Bacillus and Lysinibacillus strains from food-related sources
Exploration of surfactin production by newly isolated Bacillus and Lysinibacillus strains from food-related sources Open
As a lipopeptide (LP), surfactin exhibits properties, such as emulsifying and dispersing ability, which are useful in food industry. Discovery of new LP-producing strains from food sources is an important step towards possible application …
View article: Surfactin Shows Relatively Low Antimicrobial Activity against Bacillus subtilis and Other Bacterial Model Organisms in the Absence of Synergistic Metabolites
Surfactin Shows Relatively Low Antimicrobial Activity against Bacillus subtilis and Other Bacterial Model Organisms in the Absence of Synergistic Metabolites Open
Surfactin is described as a powerful biosurfactant and is natively produced by Bacillus subtilis in notable quantities. Among other industrially relevant characteristics, antimicrobial properties have been attributed to surfactin-producing…
View article: Expression of degQ gene and its effect on lipopeptide production as well as the formation of secretory proteases in Bacillus subtilis strains
Expression of degQ gene and its effect on lipopeptide production as well as the formation of secretory proteases in Bacillus subtilis strains Open
Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B. subtilis strains JABs24 and DSM10T, surfactin, and plipastatin are produced. Lipopeptide formation is controlled, among others, by the DegU…
View article: Expression of <i>degQ</i> gene and its effect on lipopeptide production as well as formation of secretory proteases in <i>Bacillus subtilis</i> strains
Expression of <i>degQ</i> gene and its effect on lipopeptide production as well as formation of secretory proteases in <i>Bacillus subtilis</i> strains Open
Bacillus subtilis is described as a promising production strain for lipopeptides. In the case of B . subtilis strains JABs24 and DSM10 T , surfactin and plipastatin are produced. Lipopeptide formation is controlled, among others, by the De…
View article: Bacillus subtilis High Cell Density Fermentation Using a Sporulation-Deficient Strain for the Production of Surfactin
Bacillus subtilis High Cell Density Fermentation Using a Sporulation-Deficient Strain for the Production of Surfactin Open
Bacillus subtilis 3NA is a strain capable of reaching high cell densities. A surfactin producing sfp + variant of this strain, named JABs32, was utilized in fed-batch cultivation processes. Both a glucose and an ammonia solution were fed t…
View article: Surfactin from Bacillus subtilis displays promising characteristics as O/W-emulsifier for food formulations
Surfactin from Bacillus subtilis displays promising characteristics as O/W-emulsifier for food formulations Open
In comparison to other natural and synthetic emulsifiers, the results showed that surfactin is a strong candidate to form and stabilize O/W emulsions under the reported conditions.
View article: Draft Genome Sequence of the Type Strain Bacillus subtilis subsp. <i>subtilis</i> DSM10
Draft Genome Sequence of the Type Strain Bacillus subtilis subsp. <i>subtilis</i> DSM10 Open
The Bacillus subtilis subsp. subtilis type strain DSM10 has been used as a reference in various studies. However, detailed information about the genome has not been available. Therefore, whole-genome sequencing was performed, and the seque…
View article: Towards the Anaerobic Production of Surfactin Using Bacillus subtilis
Towards the Anaerobic Production of Surfactin Using Bacillus subtilis Open
The anaerobic growth of B. subtilis to synthesize surfactin poses an alternative strategy to conventional aerobic cultivations. In general, the strong foam formation observed during aerobic processes represents a major obstacle. Anaerobic …